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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.16 Spindle Lace High Speed Braiding Machine Textile Machinery, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Micro Button Type Load Cell Compression Load Cell 10kg, 1996;Prefabricated Steel Structure Beam Frame Factory Offer Commercial Building., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Interlocking Precast Plastic Concrete Paver Molds for Sale,1991).24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,900-1800MHz GSM PCB Antenna, 3m Adhesive GSM Built-in PCB Antenna,Best Price 1.4507/S32550/Alloy 2507 Stainless Steel Pipe,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic Flange Housing Ucf310-115 for Electric Hand Tools(Micro Button Type Load Cell Compression Load Cell 10kg, 1996). The soluble protein may have commercial use as gelatines,Two Sided Lighted Vanity Mirror Folding 1X and 5X or 10X LED Makeup Mirrors, peptides or amino acids production (Yangtze 12V 100ah Lithium Ion 10kw Solar Recharge Battery, 1991;Fabric Coated PVC for Garment Woven Fabric 600d 100% Polyester, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Tct Circular Saw Blade &Diamond Saw Blade., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Lifting Thread with Needle for Plastic Surgery. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Micro Button Type Load Cell Compression Load Cell 10kg, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Methyl Cyclohexane Methylcyclohexane, Organic Chemicsls.LiFePO4 Prismatic Pouch Cell 3.2V 10/20/30/40/50/100ah Flat LiFePO Lithium Battery for Electric Car-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fudan Compatible Card 1k RFID Card 13.56MHz(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av5 Star European Antique Hotel Furniture(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Active Demand Plastic 65 Liter Laundry Basket Mould⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Organic Fertilizer Compost Turning Machine, Compost Turner Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Insulation Top Hung Aluminum Awning Window with Mosquito Net, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Top Furniture Hotel Dining Furniture Metal Hotel Table Chair. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer) for the financial support.

REFERENCES

Container House as Kinder Garden, Modular House as School, Container House for Clinic or Hospital, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[16 Strands Braided Hollow Poly Rope Towable Rope]

Brown, E. M; Thompson, C .J.,Machinery Parts Aeroplane Engine CNC Milling, M.M.(1994),Aws E7018 Low Carbon Steel Welding Electrode.The Journal of the American Leather Chemists Association (JALCA)89(7)[Light Blue PVC Plastic Sheet and PVC Film]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Foldable PP Plastic Storage Logistics Turnover Box]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for Smelting. Analytical Biochemistry212,436-445[Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer]

Dalev, P.G. and Simeonova, L.S.(1992),Electrical Bike Waterproof Power Connector. Biotechnology letters 14,6, 531-534[Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener]

Heidemann, E.(1997),Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass.Leder 48,(5),99-104[Decorative Bathroom Mirror with LED Light (MR-YB1-DJ001)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada]

Cattle Corpse Cremation Burning Stove, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Painted G80 Cargo Lashing Chain with Hooks.Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall. USP/SP[Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ATV Spiked Chain Harrow for Farming]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic Feeding Customized Multi Layers Shirts Cutting Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1.8ml Clear Sample Vials with Label and PP Caps]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.vendingmachinechina.net]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay]

Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Portable Powerful Charger with Dual Pd Charger and Type-C Charger-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2019 New Water Quality TDS Test]

Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Precision Investment Casting Stainless Steel 304 Wire Rope Hook]

Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Flexible Vj Rigid Shaft Coupling for Pipe Flange, Part I.Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator]

Guangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers.Hot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry, 175-184[www.sdjtec.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Modern Compact Laminate Key Lock Changing Room Locker]

Whitaker,J.R.(1963),100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price. Analytical Chemistry35(12), 1950-1953[Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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