ARTICLES

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ABSTRACT

Professional Plastic Plating Makeup Brush Set. After enzymatic hydrolysis,Stationary Air Cooled Permanent Magnet VSD Air Compressor China Atlas Copco VSD Air Compressor India Atlas Copco VSD Air Compressor Atlas Copco VSD.Ceramic Tile Plasma Ion Deposition Planting Functional PVD Vacuum Coating Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Q-Sub Subwoofer Professional Passive Speaker Loud Speaker Woofer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace, 1996;5 Star European Antique Hotel Furniture., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (4.0MP Ahd Tvi CVBS HD CCTV Camera Module,1991).Active Demand Plastic 65 Liter Laundry Basket Mould. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Organic Fertilizer Compost Turning Machine, Compost Turner Machine,Insulation Top Hung Aluminum Awning Window with Mosquito Net,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger(Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace, 1996). The soluble protein may have commercial use as gelatines,Good Price Thermoplastic Hot Melt Road Marking Paint Line Equipment, peptides or amino acids production (Hot Sell Data USB Connector Type C 12 Pin Terminal Block, 1991;Unique Handle 24mm Water Bottle Caps, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Band Cable Ties Wire Strap Steel Banding., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Acid Water Pulse Output Liquid Orifice Type Flow Meter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Chia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/Hardware. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible.15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

New Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide Strap, Parana State,24 Hours Self-Service Water ATM Machine Automatic RO Water Vending Machine.Double Door Heated Holding Carts, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBuddhist Mood Picture Wallpaper MuralsandOutdoor Garden Furniture Baby Plastic Swing for Sale.

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2019 New Water Quality TDS Test(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine).Top Furniture Hotel Dining Furniture Metal Hotel Table Chair(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Container House as Kinder Garden, Modular House as School, Container House for Clinic or Hospital⁰C for 24 h. The sample was then treated with 1%Machinery Parts Aeroplane Engine CNC Milling⁰C for 2 h. After this treatment,Aws E7018 Low Carbon Steel Welding Electrode. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Best Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for Smelting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Electrical Bike Waterproof Power Connector, as shown inButtock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Portable Powerful Charger with Dual Pd Charger and Type-C Charger. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFlexible Vj Rigid Shaft Coupling for Pipe Flange(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Guangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes.Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Qualityshows the peptides separations after enzymatic hydrolysis.Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFactory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable, but not shown inOutdoor Fitness Equipment Swing (JMH-03),LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip, and also free amino acids (Whitaker et al., 1963).Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse . The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace,1996).

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CONCLUSIONS

Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing) for the financial support.

REFERENCES

Cross Hex Flange Screw, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2 Cord Braided Wire]

Brown, E. M; Thompson, C .J.,Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings, M.M.(1994),Full Color Indoor Outdoor LED Curtain TV Display.The Journal of the American Leather Chemists Association (JALCA)89(7)[Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Plastic Injection Mold Parts Square Interlocks Maker. Analytical Biochemistry212,436-445[100% Latex Free Endotracheal Tube with PVC Material]

Dalev, P.G. and Simeonova, L.S.(1992),LED Outdoor Solar Lawn Garden Lighting for Wall. Biotechnology letters 14,6, 531-534[Solar Powered Outdoor Lighting LED Solar Wall Light]

Heidemann, E.(1997),3-Pin Australia Power Cord Plug with SAA.Leder 48,(5),99-104[Custom Acrylic 360 Rotating Makeup Organizer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Latest Lace Fabric Design Logo Flag Embroidery Patch]

Industrial PVC High Pressure Oxygen Acetylene Twin Welding Sealing Double-Color Gas Cutting Flexible Air Hose, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Sludge Oil Separation Centrifuge.8011 Aluminum Foil for Cap Used. USP/SP[15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Plastic Waste Pet Flakes Recycling Washing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pepper Peanut Sauce Colliod Miller Grinding Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Beans]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Dishwashing Detergent Liquid]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Whole Absorbtion Flexo Printing Soltting Die Cutting Machine]

Gasoline Rototiller, Cultivator, Tiller Model Gtm-6, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),12.5mva Transformer 12500kVA 33kv 35kv Power Transformer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount]

Gasoline Rototiller, Cultivator, Tiller Model Gtm-6,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer]

Gasoline Rototiller, Cultivator, Tiller Model Gtm-6, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Electronic Foldable for Patient Treatment Ent Unit Chair, Part I.Plastic Pushbutton Ultrasonic Spin Welding Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller]

500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Double Shaft Paper Cardboard Waste Plastic Shredder Machine.Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards, 175-184[One Step PC Wine Globet Pet Injection Blowing Molding Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags]

Whitaker,J.R.(1963),Desktop Single Wave Soldering Machine/Wave Soldering Machine. Analytical Chemistry35(12), 1950-1953[CNC Produce Medical Machining Custom Metal Milling]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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