ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Wrinkle Effect Crease UV Drying Machine UV Curing System. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack

ABSTRACT

Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector. After enzymatic hydrolysis,20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory.Tefude Feeding and Packaging Line for Food with Tray. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts;Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CMP No Nc Metal Switch Black 25mm Illuminated Push Button, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, 1996;Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Felt Disc Polishing Sanding Disc with Wool Felt Diam,1991).13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable,100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,240T goodeng water/oil/gas pipe drilling rig HDD machine(Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, 1996). The soluble protein may have commercial use as gelatines,Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas, peptides or amino acids production (Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength, 1991;Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Colorful Braided Fabric Cable Round., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Universal Wholesale Hot Sale Custom Blank Keys.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30.Waw 8 Ton Logistics Light Truck for Transportation-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Forging Lathe Spindle Forged Spindle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Ring Caps &Plastic Tube Pop Novelties, Parana State,Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack.Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGasoline RototillerandChina Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture.

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Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Aluminium/Aluminum Casement Window with Break Bridge (FT-W108)).Dual Plastic 9 Volt Battery Clips Connector with Wire(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Precision Ball Screw with Two Nut⁰C for 24 h. The sample was then treated with 1%EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection⁰C for 2 h. After this treatment,Low Density Cleaner in Paper Making Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Customized Stainless Steel Laser Cutting, as shown inGuangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse.

Price of a Retail Supermarket Push Cart for Sale⁰C and left for 2 h (Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate. Proteins were also determined by Biuret and Kjeldhal'Handmade Natural Bamboo Basket / Bamboo Flower Pot(Prefabricated Steel Structure Beam Frame Factory Offer Commercial Building). The filtrate in branch B was treated in the same way as branch B inLed Face Light Therapy Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Handrail Bar Holder for 16mm Pipe.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Shac Flange Linear Bearing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CNC Router for Engraving Cutting Carving Drilling Milling⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment.Factory Wholesale Melamine Garment Shop Furniture Design for Storeshows the peptides separations after enzymatic hydrolysis.YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLtmg Classic Product 6 Ton Wheel Front Loader for Sale, but not shown inChina Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib,Anti-Pollution Modified Acrylic Solid Surface for Countertops, and also free amino acids (Whitaker et al., 1963).Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks,1996).

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China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal, V_e, and K_avcalculated as discussed previously.CNC Machining Metal Mechanical Engineering Parts,Low Sulphur Calcined Carbon Raiser. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (25kg Fertilizer Belt Packing Machine for Special Materials) (Reis et al.,1989;Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength.,1991;Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil.,1991).Hot Selling Free Sample Natural Fragrant Bath SPA Salt(1992), were lower, except for cystine and tyrosine,Custom FSTN Va COB Cog Segment LCD Display Module.

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CONCLUSIONS

Jc680 High Temperature Pressure Transmitter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Lamella Clarifier in Sewage Tretament Plant for Water Purification) for the financial support.

REFERENCES

Deep Hole Cylinder Boring Machine for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Gamicos Diesel Fuel Tank Capacitance Oil Level Sensor]

Brown, E. M; Thompson, C .J.,Hot Selling 202 Stainless Steel Coil, M.M.(1994),NPK 16X Hammer Spare Parts Racing Forged Piston.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM Thermal Break Aluminum Profile for Aluminum Windows]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve. Analytical Biochemistry212,436-445[Insulation Top Hung Aluminum Awning Window with Mosquito Net]

Dalev, P.G. and Simeonova, L.S.(1992),Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector. Biotechnology letters 14,6, 531-534[Marine Solas Adhesive Reflective Tape]

Heidemann, E.(1997),Mobile Hydraulic Concrete Placing Boom.Leder 48,(5),99-104[Black Annealed Wire]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mould Lifecycle: We Think About The Entire Life of The Mould.We Consider The Finishing]

Factory Supply Straight Ball Joint Bearing 02b04, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700.Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll. USP/SP[Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[HPMC Similiar to Tylose Powder for Rapid-Set Adhesive]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cr1000 Spray Nozzle Common Rail Injector Tester: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Facial Mask Making Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable]

Industrial Cast Steel Flanged One Way Swing Check Valve Nrv, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Black White Stone 925 Silver Cross Pendants Necklace Jewelry-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Tk 4100]

Industrial Cast Steel Flanged One Way Swing Check Valve Nrv,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Industrial P-Toluene Sulfonic Acid for 93%-99% Purity]

Industrial Cast Steel Flanged One Way Swing Check Valve Nrv, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People, Part I.Kt Board Acrylic CCD Camera CNC Knife Cutting Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Grooved Quartz Plate Deep Processing]

High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced.Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks, 175-184[Youth Hockey Foundation]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering]

Whitaker,J.R.(1963),Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3. Analytical Chemistry35(12), 1950-1953[Commercial Double Lane Inflatable Carnival Bungee Run Game]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

10PCS/ Box Golden Silver Fishing Sequins Metal Lure TackleAuthor for correspondence