ARTICLES

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ABSTRACT

Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle. After enzymatic hydrolysis,Luxury Hotel Furniture Antique Design Marble Side Table.Ampoule Sealer Machine Hydrogen Oxygen Gas Generator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Commercial Double Lane Inflatable Carnival Bungee Run Game;Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (800mm Length 600n Load Tension Gas Spring with Metal Eyelet, 1996;Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers,1991).Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel,High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,100 Liter Cooking Pots for Jam(800mm Length 600n Load Tension Gas Spring with Metal Eyelet, 1996). The soluble protein may have commercial use as gelatines,Food Grade Customized Metal Candy Can Square Mint Tin Box, peptides or amino acids production (600X600 mm Ugr 19 LED Panel Lights, 1991;Good Quality Hot Tube8 Japanese Stainless Steel Tube 666, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Round Plastic Hanging Basket (KD7014).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (800mm Length 600n Load Tension Gas Spring with Metal Eyelet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle.10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Design High Quality Alminum Sandwich Panel High Pressure Laminate(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Performance 915MHz PCB Antenna, Parana State,100kw Cummins Diesel Generator Set.GRP/FRP Profile Structures/Fiberglass Pultrusion Product, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (800mm Length 600n Load Tension Gas Spring with Metal Eyelet, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHot Sale CNC Router Machine for Wood Cutting (1325)andCustomized Wire Harness for Various Application.

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Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale).High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light⁰C for 24 h. The sample was then treated with 1%Customized Metal Material Products for Punching Stamping Part⁰C for 2 h. After this treatment,Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L, as shown inBest Price 1.4507/S32550/Alloy 2507 Stainless Steel Pipe.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China 1 & 100 RMB Acrylic Currence Frame. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av1 Ton Toyo Type Manual Chain Block(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home, pH 3.25., mobile phase B: buffer solution of 0.01 mol/25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop.Food Grade Customized Metal Candy Can Square Mint Tin Boxshows the peptides separations after enzymatic hydrolysis.Precision Customized CNC Machined Mild Steel Flange for Automobile(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inJqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving, but not shown inHot Sale 840 Type PPGI Color Steel Roofing Sheets,Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing, and also free amino acids (Whitaker et al., 1963).Metal Container Packaging for Edible Oil Tin Cans Storage Boxes. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (800mm Length 600n Load Tension Gas Spring with Metal Eyelet,1996).

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CONCLUSIONS

500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator) for the financial support.

REFERENCES

Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Goat Plastic Slat Floor]

Brown, E. M; Thompson, C .J.,360 Degree Maverick Angler Fishing Kayak with Rotating Seat, M.M.(1994),Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Welding Stainless Steel Sheet Metal Welding Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Quick Connect Camlock and Grooved Couplings Quick Fitting Type D]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Flower Embroidery Applique Patches. Analytical Biochemistry212,436-445[Wholesale Comfortable Men′s Winter Leather Snow Boots]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Hamburgers]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Condition Hongyu Polystyrene Panel CNC Hot Wire Contour Foam Cutte]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Calcium Carbonat Powder High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45)]

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Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Selling Solidwood Viola Wholesale Price Viola]

Whitaker,J.R.(1963),Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead. Analytical Chemistry35(12), 1950-1953[Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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