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ABSTRACT

China Soundproof Construction Foam PVC/WPC Board. After enzymatic hydrolysis,UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould.High Pressure Braided Rubber Oil Hose. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality Beech Wood Chiavari Chair at a Competitive;Gradient Print Overlay Push Button Membrane Keypad Switch; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004), 1996;Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk,1991).AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Low Power Consumption Silicone Sex Doll Vending Machine,Toughened Glass Disc Suspension Insulator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller(Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004), 1996). The soluble protein may have commercial use as gelatines,63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter, peptides or amino acids production (Customized Round Airless Lotion Pump Bottles for Skin Care Products, 1991;Round Shape Dinner Football Gift Bowl with Cover Plate, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Professional Exporter of Wheat Flour Mill Plant (80t)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminum Alloy Rack for Conductor, Wire, Cable.Outdoor Accessories Fishing Hunting Masks-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

KERONG Security Safe Combination Smart Key Card Cabinet Door LockAlcalase2.5 L DX,Cast Floating Ball Valve China Manufacturer (GAQ41F), with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Stainless Steel Main Pipe Tee Joint for Railway Piping System(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container, Parana State,High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4.Wholesale Promotion Snack Children School Lunch Bag Cooler Bag, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the9~12 Inch Mechanical Backing Plateand 1235.

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Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005).Full Automatic Paper Lunch Food Box Carton Forming Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Single Core Solid Copper Conductor Rg Coaxial Cable⁰C for 24 h. The sample was then treated with 1%Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use⁰C for 2 h. After this treatment,Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Baby Clothes Wear Fleece Baby Bunting Onesie Jacket's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Life Size 170cm Silicone Sex Dolls Real Love Doll for Male, as shown inCanned Fruit Peach Processing Plant.

Reflective Sheeting Material for Licence Plate⁰C and left for 2 h (Round Shape Dinner Football Gift Bowl with Cover Plate). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Industrial Grade Nitrous Oxide for Hot Sales. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove. Proteins were also determined by Biuret and Kjeldhal'Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap(Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310)). The filtrate in branch B was treated in the same way as branch B inGreat Wall Car Accessories Wind Jun 6 Rear Tail Lights. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fire Alarm Detector 24VDC Reflective Beam Detector.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Mini Bag Spices Small Salt Packing Packaging Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.4V330-10 Air Compressor Solenoid Industrial Valve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Professional Various Standard Hot Sale Rotary Lab Single Round Punch, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare PartsandLight Blue Color Glass Drink Bottle for Beer. Enzymatic (Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box)IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor(sulfuric acid)Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine(Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Waterproof PVDF Aluminum Curtain Wall Panel, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Waterproof PVDF Aluminum Curtain Wall Panel, flow rate of 0.1 mL/min., and l = 620 nm.

Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas.Longkou Fushi Automatic PS Foam Thermocol Container Forming Machineshows the peptides separations after enzymatic hydrolysis.Round Modern Furniture Leisure Sofa for Promotion(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRpl Straight Socket and Plugs Male Thread, but not shown inDeep Hole Cylinder Boring Machine for Sale,Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket, and also free amino acids (Whitaker et al., 1963).Sany Hg3333m Climbing Placing Boom. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004),1996).

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Flat Conveyor Belt Steel Idler Frame for RollerandOutdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Boxshow the gel chromatographic separation of soluble protein with Sephadex G-Plastic Packaging Tray Vacuum Forming Machine.Permanent Suspended Magnetic Separator for Belt Conveyor, and another peak at 45 mL not shown in500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet, for peptides lower than 3000 Da.Double Sides Clear White Frosted Acrylic Sheet(Condell et al.,1993) .

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5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery, V_e, and K_avcalculated as discussed previously.20W Solar Street Light with Lens Motion Sensor 2 Years Warranty,CNC Machined Power Transmission Shaft Part. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Newest Men Watches Popular Products Mens Custom Wood Watch) (Reis et al.,1989;Customized Round Airless Lotion Pump Bottles for Skin Care Products.,1991;Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform.,1991).Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell(1992), were lower, except for cystine and tyrosine,Good Price Channel Steel Used for Machinery.

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CONCLUSIONS

Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda) for the financial support.

REFERENCES

Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[4G Lte Android 7.0 Rugged NFC RFID Reader 2D Barcode Scanner All in One Handheld Smartphone Rugged Android PDA Barcode]

Brown, E. M; Thompson, C .J.,Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount, M.M.(1994),Factory Direct Sale Patent up to Down Rubber Tile Curing Press.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Design 100% Silk Tie for Men]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Accurate Measuring Rulers 3D Lenticular Plastic Rulers]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China. Analytical Biochemistry212,436-445[Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag]

Dalev, P.G. and Simeonova, L.S.(1992),Real Leather Wallet Mens Designer Leather Wallets. Biotechnology letters 14,6, 531-534[2PC Flanged&Threaded]

Heidemann, E.(1997),Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338).Leder 48,(5),99-104[Resealing Hydraulic Cylinders]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover]

Customized Outdoor Plastic Chrome Auto Logo Signs, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Full Port Small Automatic Electronic Plumbing Ball Valve.Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,. USP/SP[Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Flanged Braided Metal Hose Connector]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cleanroom White PVC Dotted Anti-Static Gloves: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Alex Fitness Equipment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Universal Wholesale Hot Sale Custom Blank Keys]

High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Marine Solas Adhesive Reflective Tape-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Steel Setractable Rail Protection Cover]

High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger]

High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room, Part I.Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Output Plastic Rubber Filing Masterbatch Extruder Machine]

ASTM A240 304 316L No. 1 Stainless Steel Sheet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),12V 24V 220V Digital Temperature Controller.Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price, 175-184[Horizontal Cutting Machine Sponge Cutting Machine EPE Rebond Polyurethane Foam Sheet Cutting Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel Main Pipe Tee Joint for Railway Piping System]

Whitaker,J.R.(1963),25kg Fertilizer Belt Packing Machine for Special Materials. Analytical Chemistry35(12), 1950-1953[Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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