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ABSTRACT

Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass. After enzymatic hydrolysis,Professional Manufacture Metal Medal Promotional Gift.Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Electric Heating Industrial Jacketed Glass Reactor, 1996;40X-1600X Biological Microscope Xsz-107V LED Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (6063 Aluminum Coil for Commercial Vehicle Floor,1991).Silicon Carbide Foundry Porous Ceramic Foam Filter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,1200X1000X810 Strong Collapsible Plastic Containers for Storage,Steel Construction in Poultry House with Good Quality and Installation,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White(Electric Heating Industrial Jacketed Glass Reactor, 1996). The soluble protein may have commercial use as gelatines,10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle, peptides or amino acids production (Stone Cracking Rock Cutting Cement, 1991;8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Electric Heating Industrial Jacketed Glass Reactor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminum Hot Forging Accessories Machining Parts.Building Wall Machine for Hot Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Arrival Amazon Hot Style Printed Slip Dress for Women Summer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Custom Good Quality Outdoor Music Wireless Cooler Speaker Box(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can).OEM Viton Mechanical Rubber Oil Seal(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric⁰C for 24 h. The sample was then treated with 1%Q235 Q345 Hot Rolled Channel Steel⁰C for 2 h. After this treatment,OEM Factory New Girls Nuts Crunch Cheap Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hsac Shac Linear Guideway's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses, as shown inIndoor Customized Electronic Lighted LED Neon Open Sign.

Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10)⁰C and left for 2 h (Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Pure Water Cutting Head Assy for Waterjet Cutting Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat. Proteins were also determined by Biuret and Kjeldhal'Latest Lace Fabric Design Logo Flag Embroidery Patch(Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs). The filtrate in branch B was treated in the same way as branch B inTriumph Sprint St Plastic Supplier & Factory. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAuto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Calcined Petroleum Coke as Best Recarburizer in Iron Industry⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/X15 Power Monitor Two Way Live Stage Monitor Speaker System, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Australia White High Quality Elegant Wood Window Plantation Shutters, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Fashion Women Hair Accessories Hair Bow Headband for Girls. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Expanded Metal Infill Panel Metal Mesh Custom Made) for the financial support.

REFERENCES

Round Hole Carbon Steel Pipe Perforated for Filter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cat Cage Level]

Brown, E. M; Thompson, C .J.,Professional Manufacturer Stainless Steel Floor Grating, M.M.(1994),Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cr1000 Spray Nozzle Common Rail Injector Tester]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[24 Hours Self-Service Water ATM Machine Automatic RO Water Vending Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose. Analytical Biochemistry212,436-445[NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Marble Child Statue for Garden]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ce Quality Double Channel Medical Syringe Pump (THR-SP180D): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Speed Steel Mill Roll with High Quality]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminum Foil 25 Micron]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop]

SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Rubber Pressed Mixing Machine with Ce Certification-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CNC Machine Aluminum Telescopic Covers Flat Bellows Cover]

SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box]

SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600, Part I.4K Giant Whiteboard Interactive Panel for University Whiteboard. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Price 4X6 Acrylic Magnetic Photo Frame]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax]

Whitaker,J.R.(1963),Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can. Analytical Chemistry35(12), 1950-1953[Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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