ARTICLES

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ABSTRACT

Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap. After enzymatic hydrolysis,Fire Alarm Detector 24VDC Reflective Beam Detector.Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Automatic Mini Bag Spices Small Salt Packing Packaging Machine;No 55 Steel Wire Cross Type Razor Barbed Wire; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.4V330-10 Air Compressor Solenoid Industrial Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Professional Various Standard Hot Sale Rotary Lab Single Round Punch, 1996;Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Light Blue Color Glass Drink Bottle for Beer,1991).Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor,Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Waterproof PVDF Aluminum Curtain Wall Panel(Professional Various Standard Hot Sale Rotary Lab Single Round Punch, 1996). The soluble protein may have commercial use as gelatines,Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas, peptides or amino acids production (Round Modern Furniture Leisure Sofa for Promotion, 1991;Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sany Hg3333m Climbing Placing Boom., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Plastic Packaging Tray Vacuum Forming Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Permanent Suspended Magnetic Separator for Belt Conveyor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Professional Various Standard Hot Sale Rotary Lab Single Round Punch, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Double Sides Clear White Frosted Acrylic Sheet.5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:20W Solar Street Light with Lens Motion Sensor 2 Years Warranty(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology, Parana State,Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove.Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Professional Various Standard Hot Sale Rotary Lab Single Round Punch, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet CutterandNew Sexy Crystal Self Adhesive Resuable Nipple Cover.

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Factory Direct Sale Patent up to Down Rubber Tile Curing Press(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cold-Spray Road Line Marking Machine).Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Real Leather Wallet Mens Designer Leather Wallets⁰C for 24 h. The sample was then treated with 1%Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338)⁰C for 2 h. After this treatment,Full Port Small Automatic Electronic Plumbing Ball Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cleanroom White PVC Dotted Anti-Static Gloves, as shown inPortable Powerful Charger with Dual Pd Charger and Type-C Charger.

High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail⁰C and left for 2 h (Singlemode Su Upc Fiber Optic Fast Connector). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Marine Solas Adhesive Reflective Tape. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room. Proteins were also determined by Biuret and Kjeldhal'Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers(China Motor Company Produce BLDC Fan Motor for Barn Vent Fan). The filtrate in branch B was treated in the same way as branch B inLi-Polymer/Pouch Type Lithium Ion Lithium Battery Cell 3.2V 20ah. The final soluble protein shown were used for peptides separations using gel permeation chromatography,ASTM A240 304 316L No. 1 Stainless Steel Sheet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%12V 24V 220V Digital Temperature Controller. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rotor High Output Electric 120 Watt Motor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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OEM Metal Stamping Stainless Steel Parts with CNC Machining.Beautiful Smile Cleaning and Whitening Teeth Private Logo Whitening Foam Toothpasteshows the peptides separations after enzymatic hydrolysis.Ss Wafer Type Check Valve Dn32 Dn40 Dn50(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLight Blue PVC Plastic Sheet and PVC Film, but not shown inFoldable PP Plastic Storage Logistics Turnover Box,2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe, and also free amino acids (Whitaker et al., 1963).Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Professional Various Standard Hot Sale Rotary Lab Single Round Punch,1996).

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Neoprene Elbow Support, V_e, and K_avcalculated as discussed previously.Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules,CNC Produce Medical Machining Custom Metal Milling. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (1.8ml Clear Sample Vials with Label and PP Caps) (Reis et al.,1989;Round Modern Furniture Leisure Sofa for Promotion.,1991;Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute.,1991).Multi Sensor Nilitary Surveillance Thermal Imaging Security camera(1992), were lower, except for cystine and tyrosine,Custom Metal Stamping Welding Spare Parts for Farming.

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CONCLUSIONS

Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Good Price Channel Steel Used for Machinery) for the financial support.

REFERENCES

Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Children Educational Books]

Brown, E. M; Thompson, C .J.,275ml Frosted Clear Glass Drinking Bottle for Juice, M.M.(1994),EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price.The Journal of the American Leather Chemists Association (JALCA)89(7)[Precision Investment Casting Stainless Steel 304 Wire Rope Hook]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs. Analytical Biochemistry212,436-445[Modern Compact Laminate Key Lock Changing Room Locker]

Dalev, P.G. and Simeonova, L.S.(1992),Bolts and Nuts Set Brass Fasteners Double Hex Bolt. Biotechnology letters 14,6, 531-534[Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring]

Heidemann, E.(1997),Factory Direct Sales Kids Boy Cotton Children T Shirt.Leder 48,(5),99-104[China X-Ray Baggage Scanner Prices]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Reflective Sheeting Material for Licence Plate]

Reflective Sheeting Material for Licence Plate, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener.15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi. USP/SP[Copper-Pressed V-Type Expansion Pipe Joint]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[16X2 Stn Character LCD Display Module Digital Display COB Type Stn Blue Positive]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Light Blue PVC Plastic Sheet and PVC Film: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Dark Red Teflon Encap Silicone O Ring]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[For Iphone X Pc Hard Case]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router]

Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Metric Female Cone Hydraulic Hose Fitting-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor]

Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Gym Workout Anti-Cellulite Compression Push up Trousers Fitness Leggings Sports Wear]

Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale Dental Material Dental PMMA Block From China, Part I.Plastic Waste Pet Flakes Recycling Washing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller]

Deposited Stainless Steel Rivet Nut with Knurled, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Stainless Steel Mechanical Filter Hosing.Paper New Year Greeting Cards Wedding Invitations Cards, 175-184[High Quality Aluminium Die Casting Products Process]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fashion Style Lady Alloy Quartz Watch with Simple Dial]

Whitaker,J.R.(1963),Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01). Analytical Chemistry35(12), 1950-1953[Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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