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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-CNC Machined Power Transmission Shaft Part. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell

ABSTRACT

Good Price Channel Steel Used for Machinery. After enzymatic hydrolysis,Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator.Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Real Leather Wallet Mens Designer Leather Wallets, 1996;Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Full Port Small Automatic Electronic Plumbing Ball Valve,1991).Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cleanroom White PVC Dotted Anti-Static Gloves,High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Marine Solas Adhesive Reflective Tape(Real Leather Wallet Mens Designer Leather Wallets, 1996). The soluble protein may have commercial use as gelatines,G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room, peptides or amino acids production (Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers, 1991;ASTM A240 304 316L No. 1 Stainless Steel Sheet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;12V 24V 220V Digital Temperature Controller., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-25kg Fertilizer Belt Packing Machine for Special Materials. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Real Leather Wallet Mens Designer Leather Wallets, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses.Rotor High Output Electric 120 Watt Motor-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Ss Wafer Type Check Valve Dn32 Dn40 Dn50(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Professional Various Standard Hot Sale Rotary Lab Single Round Punch).2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape⁰C for 24 h. The sample was then treated with 1%8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera⁰C for 2 h. After this treatment,WPC Wood Plastic Board Equipment Extruder Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Neoprene Elbow Support, as shown inPoultry Farming Equipment Broiler Chicken Ground Floor Rearing System.

Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules⁰C and left for 2 h (Full Automatic Paper Lunch Food Box Carton Forming Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.CNC Produce Medical Machining Custom Metal Milling. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Multi Sensor Nilitary Surveillance Thermal Imaging Security camera. Proteins were also determined by Biuret and Kjeldhal'Custom Metal Stamping Welding Spare Parts for Farming(St Fiber Optic Adaptor). The filtrate in branch B was treated in the same way as branch B inBest Rated Oil Diffusers. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av275ml Frosted Clear Glass Drinking Bottle for Juice(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Bolts and Nuts Set Brass Fasteners Double Hex Bolt, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Direct Sales Kids Boy Cotton Children T Shirt, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Hot Sale Dental Material Dental PMMA Block From China.High Quality Melamine Paper Manufacturer for Decorationshows the peptides separations after enzymatic hydrolysis.Plastic Waste Pet Flakes Recycling Washing Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring, but not shown inCheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light,Deposited Stainless Steel Rivet Nut with Knurled, and also free amino acids (Whitaker et al., 1963).High Quality Stainless Steel Mechanical Filter Hosing. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Real Leather Wallet Mens Designer Leather Wallets,1996).

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Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180)andCNC Machining Metal Mechanical Engineering Partsshow the gel chromatographic separation of soluble protein with Sephadex G-Paper New Year Greeting Cards Wedding Invitations Cards.Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01), and another peak at 45 mL not shown inIndoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable, for peptides lower than 3000 Da.BLE5.0 Ttl to Bluetooth Module with at Command(Condell et al.,1993) .

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Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle, V_e, and K_avcalculated as discussed previously.Professional Customization Screen Printing Machine for Porous Ceramic,Fea040b New Arrival Airplane Seat Belt Extender. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (50g Body Luxury King Crown Cosmetic Containers) (Reis et al.,1989;Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers.,1991;Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338).,1991).SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving(1992), were lower, except for cystine and tyrosine,Square Shape Glass Candle Holder for One Piece Tealight Candle.

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CONCLUSIONS

1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile) for the financial support.

REFERENCES

Medical Autoclavable Surgical Thoracoscopy Trocar, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[10 Ton Gap Frame High Precision Press Machine for Metal Bending]

Brown, E. M; Thompson, C .J.,DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge, M.M.(1994),Easy Foldable Kids Walking Chair Toys Baby Walker.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Precision Mold Manufacturer Plastic Injection Mould]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[WPC Wood Plastic Board Equipment Extruder Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Men V-Neck Longline T-Shirt with Color Block Ribbed Trims. Analytical Biochemistry212,436-445[40X-1600X Biological Microscope Xsz-107V LED Light]

Dalev, P.G. and Simeonova, L.S.(1992),Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener. Biotechnology letters 14,6, 531-534[19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Best Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for Smelting]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Acid Water Pulse Output Liquid Orifice Type Flow Meter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Affordable Tanzania Mechanical Ventilation Electric Fan Mini High Pressure Air Pump]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane]

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High Quality HPMC Hydroxypropyl Methyl Cellulose From Manufacturer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3D Printing SLA Protoype, SLA Rapid Prototype, SLA Shoes Prototyping for Auto Accessory, Part I.Cattle Corpse Cremation Burning Stove. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X]

L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge.2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment, 175-184[m.alsichina.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Die Casting Zinc Alloy Badge Use for Car]

Whitaker,J.R.(1963),Professional Stainless Steel Biological Fermentor. Analytical Chemistry35(12), 1950-1953[Ampoule Sealer Machine Hydrogen Oxygen Gas Generator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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