ARTICLES

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ABSTRACT

Colorful Braided Fabric Cable Round. After enzymatic hydrolysis,Universal Wholesale Hot Sale Custom Blank Keys.Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30;Waw 8 Ton Logistics Light Truck for Transportation; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Forging Lathe Spindle Forged Spindle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale Chic Straw Braid Floppy Hat with Print Tape, 1996;High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lamella Clarifier in Sewage Tretament Plant for Water Purification,1991).BMC (DMC) / SMC Compression Moulding. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ring Caps &Plastic Tube Pop Novelties,Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk(Hot Sale Chic Straw Braid Floppy Hat with Print Tape, 1996). The soluble protein may have commercial use as gelatines,Dual Plastic 9 Volt Battery Clips Connector with Wire, peptides or amino acids production (Precision Ball Screw with Two Nut, 1991;EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Low Density Cleaner in Paper Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Customized Stainless Steel Laser Cutting. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale Chic Straw Braid Floppy Hat with Print Tape, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Price of a Retail Supermarket Push Cart for Sale.Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape, Parana State,Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen.CNC Router for Engraving Cutting Carving Drilling Milling, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hot Sale Chic Straw Braid Floppy Hat with Print Tape, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324andAutomatic Bread Crumb Processing Line and Making Extruder Machine.

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Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Solid Tungsten Carbide Rods).Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer⁰C for 24 h. The sample was then treated with 1%High Quality Hot Rolled Steel U Channel China Supplier⁰C for 2 h. After this treatment,M Type Metallic Yarn Lurex Yarn Embroidery Yarn. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck, as shown inCold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052.

500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment⁰C and left for 2 h (Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Anti-Pollution Modified Acrylic Solid Surface for Countertops. Proteins were also determined by Biuret and Kjeldhal'Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose(Custom Steel Investment Casting High Hardness Agricultural Machinery Parts). The filtrate in branch B was treated in the same way as branch B inWaterproof Adjustable Passive RFID Wristband price Silicone RFID Wristband / Bracelet NFC TAG  MIFARE Ultralight EV1 /MIFARE Ultralight C RFID silicone Bracelet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Corrugated Carton Box Fully Automatic Folding Gluing Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%800lb 1/2 A105 Thread Forged Steel Globe Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avKorea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CNC Machining Metal Mechanical Engineering Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Low Sulphur Calcined Carbon Raiser, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Selling Free Sample Natural Fragrant Bath SPA Salt, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve.Samsung LTI550HN16 1920X1080P monitor screen LCD video wallshows the peptides separations after enzymatic hydrolysis.Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inElectrical Bike Waterproof Power Connector, but not shown inEnviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels,Mobile Hydraulic Concrete Placing Boom, and also free amino acids (Whitaker et al., 1963).Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Sale Chic Straw Braid Floppy Hat with Print Tape,1996).

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Black White Stone 925 Silver Cross Pendants Necklace Jewelry, V_e, and K_avcalculated as discussed previously.Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People,Kt Board Acrylic CCD Camera CNC Knife Cutting Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Calcined Petroleum Coke as Best Recarburizer in Iron Industry) (Reis et al.,1989;Precision Ball Screw with Two Nut.,1991;High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply.,1991).High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17(1992), were lower, except for cystine and tyrosine,Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced.

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CONCLUSIONS

Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Shac Flange Linear Bearing) for the financial support.

REFERENCES

Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Solar Panel 1000w]

Brown, E. M; Thompson, C .J.,China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part, M.M.(1994),Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box.The Journal of the American Leather Chemists Association (JALCA)89(7)[Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[6063 Aluminum Coil for Commercial Vehicle Floor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz. Analytical Biochemistry212,436-445[Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People]

Dalev, P.G. and Simeonova, L.S.(1992),Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator. Biotechnology letters 14,6, 531-534[Circular DC Electro Lifting Magnet for Lifting Scraps]

Heidemann, E.(1997),Carbon Steel Stainless Steel Countersunk Head Hollow Bolt.Leder 48,(5),99-104[Food Grade Microwavable Takeaway Clear Recycled Plastic Fruit Single Lunch Disposable PP Kitchen Fast Food Packaging Plastic Food Container with Lids]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wood Activated Carbon for Decolorizing in Food Industry with ASTM]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality 3]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fea040b New Arrival Airplane Seat Belt Extender]

5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Clear PVC Roof Frame Tent in South Africa-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ 5g]

5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cast Floating Ball Valve China Manufacturer (GAQ41F)]

5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511), Part I.Stainless Steel Bend Pipe for Industrial Use. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape]

Ybz-E16f20 Vane Steering Hydraulic Pumps, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Good Quality Snow White Pumpkin Seeds for Sale.ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors, 175-184[www.nsen-valve.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl]

Whitaker,J.R.(1963),Automotive Acrylic Wholesale Masking Tape Factory Price. Analytical Chemistry35(12), 1950-1953[Industrial Cast Steel Flanged One Way Swing Check Valve Nrv]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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