ARTICLES

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ABSTRACT

Building Material Sound Heat Insulation Rock Wool Sandwich Panel. After enzymatic hydrolysis,Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration.Ltmg Classic Product 6 Ton Wheel Front Loader for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Samsung LTI550HN16 1920X1080P monitor screen LCD video wall, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts, 1996;Household Family Medical Emergency Electronic Digital Thermometer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Die Cast Iron Worm Gear Speed Reduce Gearbox,1991).19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Couple Watches Steel Material Gift Watch OEM in China,Glass Tea Sugar Coffee Storage Jar with Screw Cap,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Circular DC Electro Lifting Magnet for Lifting Scraps(Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts, 1996). The soluble protein may have commercial use as gelatines,Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts, peptides or amino acids production (Particle Board Manufacturer, Melamine/Plain Chipboard, 1991;CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰120ml Glass Perfume Bottles for Designer Perfume.Agriculture Hay Silage Wrapping Film-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Air Purification Activated Alumina).LED Rectangular Recessed Linear Lamps for Bank and Car Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Salon Equipment Hair Clipper⁰C for 24 h. The sample was then treated with 1%Flat Conveyor Belt Steel Idler Frame for Roller⁰C for 2 h. After this treatment,LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Durable TPR Pet Molar Toy with Colorful Rope, as shown in100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRoller Hearth Heating Furnace for Gas Cylindrer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hotel Card Door Lock Access Control Safe Door Lock, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Stainless Steel Balustrade/Pillar/Column. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Large Outdoor Simple Gazebo Beer Festival Marquee Tent) for the financial support.

REFERENCES

Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Popular Outdoor Canopy Party Tent Gazebo Pavilion Cater Events]

Brown, E. M; Thompson, C .J.,PP/Pet Woven Geotextile for Road Construction, M.M.(1994),Lamella Clarifier in Industrial Wastewater Treatment.The Journal of the American Leather Chemists Association (JALCA)89(7)[Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering. Analytical Biochemistry212,436-445[LED Bulb Light UFO Light Recruitment Distributor]

Dalev, P.G. and Simeonova, L.S.(1992),Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED. Biotechnology letters 14,6, 531-534[High Quality Women′s Suit in Reasonable Price]

Heidemann, E.(1997),Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights.Leder 48,(5),99-104[Stainless Steel Lag Screw]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hsac Shac Linear Guideway]

NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick.Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts. USP/SP[FRP Pultruded Pipe or GRP Profile]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-IS Type Cast Iron Chemical Industrial Pump: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1t Commercial Automatic Ice Machine Flake Ice Making Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Large Outdoor Simple Gazebo Beer Festival Marquee Tent]

Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Grooved Quartz Plate Deep Processing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Couple Watches Steel Material Gift Watch OEM in China]

Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Rigid PVC Foam Board for FRP Marine]

Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale CNC Router Machine for Wood Cutting (1325), Part I.Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Mini LED Flood Light SMD Flood Light 50W]

50g Body Luxury King Crown Cosmetic Containers, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men.Mini Succulent Planters, Cactus Pots with Wooden Cement Pot, 175-184[Packaging Film Companies]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[4 Inch Quarter Round PCD Diamond Grinding Cup Wheel]

Whitaker,J.R.(1963),Paper Egg Box Forming Molding Production Line/ Egg Tray Machine. Analytical Chemistry35(12), 1950-1953[Customized Printed Decorative Mailing Bubble Bags in Bright Yellow Color for Envelopes Packaging]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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