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ABSTRACT

Unique Handle 24mm Water Bottle Caps. After enzymatic hydrolysis,Stainless Steel Band Cable Ties Wire Strap Steel Banding.Acid Water Pulse Output Liquid Orifice Type Flow Meter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Chia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/Hardware;Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment, 1996;High Efficiency Scc638 Water-Based Cutting Fluid with Factory Production., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen,1991).High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Wholesale 1080P HDMI Male to VGA HD15p Female Adapter for PC Tablet Nickel/Gold-Plated HDMI+VGA Connector,New Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide Strap,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Double Door Heated Holding Carts(Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment, 1996). The soluble protein may have commercial use as gelatines,2019 New Water Quality TDS Test, peptides or amino acids production (Top Furniture Hotel Dining Furniture Metal Hotel Table Chair, 1991;Container House as Kinder Garden, Modular House as School, Container House for Clinic or Hospital, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Machinery Parts Aeroplane Engine CNC Milling., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aws E7018 Low Carbon Steel Welding Electrode.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Best Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for Smelting. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Electrical Bike Waterproof Power Connector.Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Painted G80 Cargo Lashing Chain with Hooks(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Guangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Rht-060ws Soda Process Water Cooled Screw Type Water Chiller).Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry⁰C for 24 h. The sample was then treated with 1%100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price⁰C for 2 h. After this treatment,15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Speed Steel Mill Roll with High Quality's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer, as shown inNew Electric Piston Pump Coating Machine PT6900.

2017 China Hot Sale Water Screen Movie Fountain⁰C and left for 2 h (Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor. Proteins were also determined by Biuret and Kjeldhal'LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip(Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace). The filtrate in branch B was treated in the same way as branch B inCL2T-SF304N Comlom Nylon Grip Handle Military Folding Shovel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse .

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Individually Paper Wrapped Biodegradable Paper Drinking Straws Wholesale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLatest New Hho Engine Oil Carbon Remover(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Delicate Handmade Round Natural Stone Chain Necklace⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Full Color Indoor Outdoor LED Curtain TV Display.Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Useshows the peptides separations after enzymatic hydrolysis.High Quality Plastic Injection Mold Parts Square Interlocks Maker(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Crusher for Used Plastic Recycling Line, but not shown inOutdoor Accessories Fishing Hunting Masks,LED Outdoor Solar Lawn Garden Lighting for Wall, and also free amino acids (Whitaker et al., 1963).3-Pin Australia Power Cord Plug with SAA. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment,1996).

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CONCLUSIONS

Double Shaft Paper Cardboard Waste Plastic Shredder Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts) for the financial support.

REFERENCES

Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm]

Brown, E. M; Thompson, C .J.,Desktop Single Wave Soldering Machine/Wave Soldering Machine, M.M.(1994),Manufacturers White Dinnerware Set Fast Food Tray Bento Box.The Journal of the American Leather Chemists Association (JALCA)89(7)[Tungsten Solid Carbide Straight 4/6 Flutes Reamer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[IP65 Outdoor Solar Flood Lamp with Battery for Commercial]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Price Graphite Electrode HP 300mm UHP. Analytical Biochemistry212,436-445[CNC Metal Grinding Machine Tool Mk2120]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1. Biotechnology letters 14,6, 531-534[ High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product]

Heidemann, E.(1997),Wld8200 Auto Spray Paint Booth.Leder 48,(5),99-104[Electric Commode Wheelchair]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[6090 CO2 Sealed Glass Cooling Laser Cutting Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[5.5X19mm Hex Head Self Tapping Screws with Washer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Professional Customization Screen Printing Machine for Porous Ceramic]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Contract PCB Assembly]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quaity China Supplier Truck Trailer Auto Car Accessories]

High Density Crust Foam Board/Celuka Board, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Mango Pulp Juice Processing Production Line-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering]

High Density Crust Foam Board/Celuka Board,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Professional Exporter of Wheat Flour Mill Plant (80t)]

High Density Crust Foam Board/Celuka Board, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Laser Cut Sheet Metal Fiber Laser Cutting Machine, Part I.Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ce Quality Double Channel Medical Syringe Pump (THR-SP180D)]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter]

Whitaker,J.R.(1963),130kw 7 Degrees Scroll Type Industrial Water Chiller. Analytical Chemistry35(12), 1950-1953[Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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