ARTICLES

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ABSTRACT

Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder. After enzymatic hydrolysis,Mexico Single Expansion Anchor with YZP.Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet;Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Whole Absorbtion Flexo Printing Soltting Die Cutting Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Price 4X6 Acrylic Magnetic Photo Frame, 1996;200m Diesel Oil Hydraulic Water Well Drilling Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Cmyk Printing Cake Gift Packing Box with Lid,1991).Air Purification Activated Alumina. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6),Resistan UV Grades PP Non Woven Fabric,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Scba Breathing Apparatus Equipment with Ce Certification(Factory Price 4X6 Acrylic Magnetic Photo Frame, 1996). The soluble protein may have commercial use as gelatines,FRP Pultruded Pipe or GRP Profile, peptides or amino acids production (6090 CO2 Sealed Glass Cooling Laser Cutting Machine, 1991;Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Outdoor Fitness Equipment Swing (JMH-03).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sheet Metal Distribution Cabinet with High Quality. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Price 4X6 Acrylic Magnetic Photo Frame, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk.New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Welded Stud Anchor Chain for Ship(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen).SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Line Scan Uvss System Portable Under Vehicle Surveillance⁰C for 24 h. The sample was then treated with 1%Folding Steel Manual Wheelchair with Chrome Frame⁰C for 2 h. After this treatment,Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fashion Style Lady Alloy Quartz Watch with Simple Dial's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Rooftop HVAC Packaged Units (RTU) Amrta Manufacture, as shown in900-1800MHz GSM PCB Antenna.

Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box⁰C and left for 2 h (Q-Sub Subwoofer Professional Passive Speaker Loud Speaker Woofer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Building Decoration Material Acrylic Dispersant for Coating. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine. Proteins were also determined by Biuret and Kjeldhal'Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades(Neodymium Magnet Pot Assembly with Strong Weight). The filtrate in branch B was treated in the same way as branch B inm.dmgstainless.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory Supply Straight Ball Joint Bearing 02b04.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wrinkle Effect Crease UV Drying Machine UV Curing System. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Tefude Feeding and Packaging Line for Food with Tray, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Hot Forging Lathe Spindle Forged Spindle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine) for the financial support.

REFERENCES

Hot Sale Chic Straw Braid Floppy Hat with Print Tape, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Single Control Bladder Hydraulic Tyre Curing Press]

Brown, E. M; Thompson, C .J.,High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply, M.M.(1994),Lamella Clarifier in Sewage Tretament Plant for Water Purification.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale 840 Type PPGI Color Steel Roofing Sheets]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ybz-E16f20 Vane Steering Hydraulic Pumps]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-BMC (DMC) / SMC Compression Moulding. Analytical Biochemistry212,436-445[Colored Silicone NBR O-Rings Seals]

Dalev, P.G. and Simeonova, L.S.(1992),Ring Caps &Plastic Tube Pop Novelties. Biotechnology letters 14,6, 531-534[Electronic Foldable for Patient Treatment Ent Unit Chair]

Heidemann, E.(1997),Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet.Leder 48,(5),99-104[m.4nightsz.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Balustrade/Pillar/Column]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Foska Mini Size High Quality Heavy Duty Stapler]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Precision Ball Screw with Two Nut: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Performance 915MHz PCB Antenna]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Custom Rayon Single Gold/Royalblue Intertwined Honor Cord Tassel for Graduation]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail]

EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Low Density Cleaner in Paper Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Welding Stainless Steel Sheet Metal Welding Parts]

EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Quick Connect Camlock and Grooved Couplings Quick Fitting Type D]

EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada, Part I.Customized Stainless Steel Laser Cutting. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Comfortable Men′s Winter Leather Snow Boots]

Price of a Retail Supermarket Push Cart for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable.OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate, 175-184[hnachina]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Laser ID Card Rigid PET Printing Sheet]

Whitaker,J.R.(1963),Handmade Natural Bamboo Basket / Bamboo Flower Pot. Analytical Chemistry35(12), 1950-1953[ Hamburgers]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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