ARTICLES

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ABSTRACT

300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve. After enzymatic hydrolysis,Plastic Injection Molding Companies China for Tablet PC Case.27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Tensisle Strength Tissue Paper, 1996;Tractor Mounted Beach Cleaning Equipment Large Size., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap,1991).Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PP Coated Pipe Machine Extruder Machine,China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances(High Tensisle Strength Tissue Paper, 1996). The soluble protein may have commercial use as gelatines,Mining Machinery Parts Diamond Saw Blade Segments for Marble, peptides or amino acids production (Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface, 1991;Multi Column Load Cell 100kg, 20t, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Foldable PP Plastic Storage Logistics Turnover Box. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Tensisle Strength Tissue Paper, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005.Buddhist Mood Picture Wallpaper Murals-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Flange Dismantling Joint(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine).Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Kubota Combine Harvester Spare Parts 5h484-2392 Nut⁰C for 24 h. The sample was then treated with 1%Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable⁰C for 2 h. After this treatment,HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Steel Floor Deck Post Cutting Cold Roll Forming Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180), as shown inHot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality.

Fluorous Rubber Coated Fiberglass Cloth⁰C and left for 2 h (Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern). Proteins were also determined by Biuret and Kjeldhal'PS Foam Disposable Fast Food Box Making Machine(Outdoor Fitness Equipment Swing (JMH-03)). The filtrate in branch B was treated in the same way as branch B inMooring Chain. The final soluble protein shown were used for peptides separations using gel permeation chromatography,K Helical Bevel Gearboxes Reducer Motor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Indoor Customized Electronic Lighted LED Neon Open Sign. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEcofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Free Logo Design Cheap Hand Flag (NF01F02021)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

4K Giant Whiteboard Interactive Panel for University WhiteboardandRJ45 Cat5e in-Line Coupler Modular Jack. Enzymatic (Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture)Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper(sulfuric acid)Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052(High Tensisle Strength Tissue Paper, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Disposable Blood Transfusion Set with Filter, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Disposable Blood Transfusion Set with Filter, flow rate of 0.1 mL/min., and l = 620 nm.

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Junior Backyard Training Practise Soccer Goal Net, V_e, and K_avcalculated as discussed previously.350mm Diamond Blade for Granite,Custom Welding Stainless Steel Sheet Metal Welding Parts. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors) (Reis et al.,1989;Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface.,1991;Tractor Mounted Beach Cleaning Equipment Large Size.,1991).Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp(1992), were lower, except for cystine and tyrosine,Laser ID Card Rigid PET Printing Sheet.

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CONCLUSIONS

New Arrival Plush Animal Ride for Kids for Shopping Center. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine) for the financial support.

REFERENCES

Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Electralog Electric Fireplace]

Brown, E. M; Thompson, C .J.,(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine, M.M.(1994),Ss316 Stainless Steel Heavy Duty Straps.The Journal of the American Leather Chemists Association (JALCA)89(7)[Latest Lace Fabric Design Logo Flag Embroidery Patch]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus. Analytical Biochemistry212,436-445[Plastic Waste Pet Flakes Recycling Washing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),High Temperature Side Opening AAC Autoclave, AAC Block Making Machine. Biotechnology letters 14,6, 531-534[ Beans]

Heidemann, E.(1997),PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap.Leder 48,(5),99-104[Butter Making Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Whole Absorbtion Flexo Printing Soltting Die Cutting Machine]

Automatic Bread Crumb Processing Line and Making Extruder Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation.Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal. USP/SP[Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[LED Highbay Light Warehouse Lighting 100W LED UFO Highbay Light]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags]

New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Green Color PVC Anti Dazzle Panel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CNC Produce Medical Machining Custom Metal Milling]

New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05]

New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator, Part I.XL-21 Motive Electrical Switchgear Power Cabinet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Micro Button Type Load Cell Compression Load Cell 10kg]

Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Furniture Grade E1 Glue Plywood with Competitive Price.High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn, 175-184[Waterproof New Desigen 10A 12V 20A Solar Charge Controller]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Lifting Thread with Needle for Plastic Surgery]

Whitaker,J.R.(1963),TUV Certificate High Quality Single Core PV1-F PV Solar Cables. Analytical Chemistry35(12), 1950-1953[Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

BLE5.0 Ttl to Bluetooth Module with at CommandAuthor for correspondence