ARTICLES

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ABSTRACT

5.5X19mm Hex Head Self Tapping Screws with Washer. After enzymatic hydrolysis,Industrial PVC High Pressure Oxygen Acetylene Twin Welding Sealing Double-Color Gas Cutting Flexible Air Hose.Low Volume Hard Tool Kit Thermoplastic Polymer Injection Molding. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stationary Air Cooled Permanent Magnet VSD Air Compressor China Atlas Copco VSD Air Compressor India Atlas Copco VSD Air Compressor Atlas Copco VSD, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ceramic Tile Plasma Ion Deposition Planting Functional PVD Vacuum Coating Machine, 1996;Road Construction Non Woven Needle Punched PP Non Woven Geotextile., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Scented Flavor Flower Herbal Tea,1991).Q-Sub Subwoofer Professional Passive Speaker Loud Speaker Woofer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace,5 Star European Antique Hotel Furniture,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,4.0MP Ahd Tvi CVBS HD CCTV Camera Module(Ceramic Tile Plasma Ion Deposition Planting Functional PVD Vacuum Coating Machine, 1996). The soluble protein may have commercial use as gelatines,Active Demand Plastic 65 Liter Laundry Basket Mould, peptides or amino acids production (Organic Fertilizer Compost Turning Machine, Compost Turner Machine, 1991;Insulation Top Hung Aluminum Awning Window with Mosquito Net, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Price Thermoplastic Hot Melt Road Marking Paint Line Equipment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sell Data USB Connector Type C 12 Pin Terminal Block. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ceramic Tile Plasma Ion Deposition Planting Functional PVD Vacuum Coating Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Unique Handle 24mm Water Bottle Caps.Stainless Steel Band Cable Ties Wire Strap Steel Banding-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Acid Water Pulse Output Liquid Orifice Type Flow Meter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Disposable Blood Transfusion Set with Filter).Factory Wholesale 1080P HDMI Male to VGA HD15p Female Adapter for PC Tablet Nickel/Gold-Plated HDMI+VGA Connector(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide Strap⁰C for 24 h. The sample was then treated with 1%Double Door Heated Holding Carts⁰C for 2 h. After this treatment,2019 New Water Quality TDS Test. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Top Furniture Hotel Dining Furniture Metal Hotel Table Chair's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Container House as Kinder Garden, Modular House as School, Container House for Clinic or Hospital, as shown inCustom FSTN Va COB Cog Segment LCD Display Module.

Machinery Parts Aeroplane Engine CNC Milling⁰C and left for 2 h (Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aws E7018 Low Carbon Steel Welding Electrode. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Best Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for Smelting. Proteins were also determined by Biuret and Kjeldhal'Electrical Bike Waterproof Power Connector(Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine). The filtrate in branch B was treated in the same way as branch B inAntibacterial Conductive Polyester Textile Yarn Factory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Painted G80 Cargo Lashing Chain with Hooks. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Portable Powerful Charger with Dual Pd Charger and Type-C Charger, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Flexible Vj Rigid Shaft Coupling for Pipe Flange, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine.Nbm High Quality Auto Part Factory Price 6pk2310shows the peptides separations after enzymatic hydrolysis.High Speed Steel Mill Roll with High Quality(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSwf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formations, but not shown inFood Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder,3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer, and also free amino acids (Whitaker et al., 1963).2017 China Hot Sale Water Screen Movie Fountain. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Ceramic Tile Plasma Ion Deposition Planting Functional PVD Vacuum Coating Machine,1996).

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Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse , V_e, and K_avcalculated as discussed previously.Individually Paper Wrapped Biodegradable Paper Drinking Straws Wholesale,Latest New Hho Engine Oil Carbon Remover. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Black White Stone 925 Silver Cross Pendants Necklace Jewelry) (Reis et al.,1989;Organic Fertilizer Compost Turning Machine, Compost Turner Machine.,1991;Road Construction Non Woven Needle Punched PP Non Woven Geotextile.,1991).Wood Chips Rotary Dryer Sawdust Pellet Drying Machine(1992), were lower, except for cystine and tyrosine,Delicate Handmade Round Natural Stone Chain Necklace.

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CONCLUSIONS

Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay) for the financial support.

REFERENCES

Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Home Battery Scheme]

Brown, E. M; Thompson, C .J.,Embossed Aluminium/ Aluminum Household Foil 8011-O, M.M.(1994),High Quality Aluminium Foil for Pharmaceutical, Container, Household, Lamination, Embossing, Sealing, Coating (A8011, 1235, 11100, 8079, 8021).The Journal of the American Leather Chemists Association (JALCA)89(7)[Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CREE Chip 24W 28W 30W LED Downlight]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle. Analytical Biochemistry212,436-445[FC Bare Fiber Optic Adapter for Network]

Dalev, P.G. and Simeonova, L.S.(1992),Cross Hex Flange Screw. Biotechnology letters 14,6, 531-534[Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable]

Heidemann, E.(1997),Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings.Leder 48,(5),99-104[Automatic 5gallon Bottled Shrink Sleeve Labeling Machine for Pet PVC Bottle Label]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-LED Outdoor Solar Lawn Garden Lighting for Wall: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Advertising Hologram]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence]

3-Pin Australia Power Cord Plug with SAA, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Sludge Oil Separation Centrifuge-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration]

3-Pin Australia Power Cord Plug with SAA,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Outdoor Multifunctional Solar Power Flashlight (SYSG-517)]

3-Pin Australia Power Cord Plug with SAA, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:8011 Aluminum Foil for Cap Used, Part I.Pepper Peanut Sauce Colliod Miller Grinding Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cross Hex Flange Screw]

Gasoline Rototiller, Cultivator, Tiller Model Gtm-6, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),12.5mva Transformer 12500kVA 33kv 35kv Power Transformer.Electronic Foldable for Patient Treatment Ent Unit Chair, 175-184[Conveyor Frame Drawings]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Small Coil Compression Spring in High Quality]

Whitaker,J.R.(1963),Plastic Pushbutton Ultrasonic Spin Welding Machine. Analytical Chemistry35(12), 1950-1953[Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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