ARTICLES

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ABSTRACT

En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada. After enzymatic hydrolysis,Customized Stainless Steel Laser Cutting.Price of a Retail Supermarket Push Cart for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable;OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Handmade Natural Bamboo Basket / Bamboo Flower Pot, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Handrail Bar Holder for 16mm Pipe, 1996;Shac Flange Linear Bearing., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price,1991).Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC Router for Engraving Cutting Carving Drilling Milling,Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa(Stainless Steel Handrail Bar Holder for 16mm Pipe, 1996). The soluble protein may have commercial use as gelatines,Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer, peptides or amino acids production (High Quality Hot Rolled Steel U Channel China Supplier, 1991;M Type Metallic Yarn Lurex Yarn Embroidery Yarn, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Handrail Bar Holder for 16mm Pipe, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable.Anti-Pollution Modified Acrylic Solid Surface for Countertops-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

CNC Machining Metal Mechanical Engineering Parts, Parana State,Low Density Cleaner in Paper Making Machine.Low Sulphur Calcined Carbon Raiser, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Stainless Steel Handrail Bar Holder for 16mm Pipe, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the Metal Polishing Discand Container House for Clinic or Hospital.

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Hot Selling Free Sample Natural Fragrant Bath SPA Salt(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry).Custom FSTN Va COB Cog Segment LCD Display Module(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Jc680 High Temperature Pressure Transmitter⁰C for 24 h. The sample was then treated with 1%Deep Hole Cylinder Boring Machine for Sale⁰C for 2 h. After this treatment,Hot Selling 202 Stainless Steel Coil. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)NPK 16X Hammer Spare Parts Racing Forged Piston's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve, as shown in300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Industrial Cast Steel Flanged One Way Swing Check Valve Nrv. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBlack White Stone 925 Silver Cross Pendants Necklace Jewelry(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Kt Board Acrylic CCD Camera CNC Knife Cutting Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator.NPT Standard Malleable Iron Pipe Fitting Hexagon Nippleshows the peptides separations after enzymatic hydrolysis.Carbon Steel Stainless Steel Countersunk Head Hollow Bolt(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Good Quality Outdoor Music Wireless Cooler Speaker Box, but not shown in Marble Child Statue for Garden,Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses, and also free amino acids (Whitaker et al., 1963).China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Stainless Steel Handrail Bar Holder for 16mm Pipe,1996).

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High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511), V_e, and K_avcalculated as discussed previously.Stainless Steel Bend Pipe for Industrial Use,Ybz-E16f20 Vane Steering Hydraulic Pumps. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box) (Reis et al.,1989;High Quality Hot Rolled Steel U Channel China Supplier.,1991;Shac Flange Linear Bearing.,1991).Good Quality Snow White Pumpkin Seeds for Sale(1992), were lower, except for cystine and tyrosine,ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors.

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CONCLUSIONS

Automotive Acrylic Wholesale Masking Tape Factory Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence) for the financial support.

REFERENCES

Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Building Material HPMC Hydroxypropyl Methyl Cellulose]

Brown, E. M; Thompson, C .J.,Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper, M.M.(1994),Watermrk Drop in Bathroom Basin Sink.The Journal of the American Leather Chemists Association (JALCA)89(7)[Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wld8200 Auto Spray Paint Booth]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM ABS Industrial Plastic Sheet Injection Auto Part. Analytical Biochemistry212,436-445[Biodegradable Disposible Straw Pulp Food Box Ppp-1007]

Dalev, P.G. and Simeonova, L.S.(1992),15L Big Cooling Cylinder High Production Hard Ice Cream Machine. Biotechnology letters 14,6, 531-534[NPK 16X Hammer Spare Parts Racing Forged Piston]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1 Ton Toyo Type Manual Chain Block]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customized Outdoor Plastic Chrome Auto Logo Signs: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality HPMC Hydroxypropyl Methyl Cellulose From Manufacturer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China 4X8 Feet PVC Rigid Sheet for Industrial Use]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Price of a Retail Supermarket Push Cart for Sale]

Small Table CNC Marble Engraver for Stone, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3bbl Brite Servicing Tank Beer Brewery Equipment Unitank]

Small Table CNC Marble Engraver for Stone,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[130kw 7 Degrees Scroll Type Industrial Water Chiller]

Small Table CNC Marble Engraver for Stone, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Soundproof Construction Foam PVC/WPC Board, Part I.UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat]

High Pressure Braided Rubber Oil Hose, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Beech Wood Chiavari Chair at a Competitive.Gradient Print Overlay Push Button Membrane Keypad Switch, 175-184[Work Table]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Methyl Cyclohexane Methylcyclohexane]

Whitaker,J.R.(1963),Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X. Analytical Chemistry35(12), 1950-1953[Sinotruk-HOWO 6X4 Tractor Truck]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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