ARTICLES

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ABSTRACT

Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody. After enzymatic hydrolysis,Fashionable Natural Bamboo Wood Sunglasses for Men.Solar Powered Outdoor Lighting LED Solar Wall Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM Thermal Break Aluminum Profile for Aluminum Windows, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Aluminum Window System Thermal Break Strip (HK Stype35.3mm), 1996;LED Bulb Light UFO Light Recruitment Distributor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ozone Generator Plant for Water Treatment,1991).En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Free Assembly, Walking Machine, Home Fitness, Treadmill,CMC Transparent Grade for Coating Production,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine(Aluminum Window System Thermal Break Strip (HK Stype35.3mm), 1996). The soluble protein may have commercial use as gelatines,Cold-Spray Road Line Marking Machine, peptides or amino acids production (Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner, 1991;Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2019 New Tuya Smart Wireless WiFi Smart Socket (PST-J2).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Aluminum Window System Thermal Break Strip (HK Stype35.3mm), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Rock Tunnel Boring Machine for Natural Gas Pipeline.NPK+Te Fertilizer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Gear Rack and Pinion for Construction Hoist, Construction Spare Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Steel Setractable Rail Protection Cover).Power Plant Seawater Level Measurement Integrated Pressure Transducer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Melamine Paper Manufacturer for Decoration⁰C for 24 h. The sample was then treated with 1%2019 Earn Money Cabinet Games Coin Operated Tekken 7 Retro Japanese⁰C for 2 h. After this treatment,Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Tungsten Solid Carbide Straight 4/6 Flutes Reamer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Quality Heavy Duty Globe Control Valve, as shown in4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger.

Customized Wire Harness for Various Application⁰C and left for 2 h (High Output Plastic Rubber Filing Masterbatch Extruder Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cheap CNC Spindle Peeling Lathe for Steel Bar. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Nonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elements. Proteins were also determined by Biuret and Kjeldhal'3 Years Warranty 3.2V 60ah 75ah 86ah 100ah 110ah 200ah Single LiFePO4 Battery Cell LiFePO4 Lithium Iron Phosphate Battery Cell for EV, E-Cars with Grade a(Stainless Steel Main Pipe Tee Joint for Railway Piping System). The filtrate in branch B was treated in the same way as branch B inOutdoor IP67 Aluminum Waterproof Enclosure Aluminum Box Case. The final soluble protein shown were used for peptides separations using gel permeation chromatography,6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sc LC FC St Fiber Optical Adaptor Coupler Adapter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEnt Treatment Medical Chair with Foot Control(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Leaf Shape Pearl Jewelry Wedding Tiara⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Starter Motor 0001367034 12V 3KW 9T for Mercedes, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Condition Hongyu Polystyrene Panel CNC Hot Wire Contour Foam Cutte, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Large Diaper Bag Backpack, Mokaloo Anti-Water Maternity Nappy Bags Changing Bags with Insulated Pockets and Stroller.600X600 mm Ugr 19 LED Panel Lightsshows the peptides separations after enzymatic hydrolysis.Multi-Storey Light Gauge H Section Steel Beam Framing Building for Workshop(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts, but not shown inCustom Free Logo Design Cheap Hand Flag (NF01F02021),Water Chiller Air Cooled for Cooling Beer Refrigerating, and also free amino acids (Whitaker et al., 1963).High Quality Cheap Price Disposable IV Infusion Set with Precise Filter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Aluminum Window System Thermal Break Strip (HK Stype35.3mm),1996).

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CONCLUSIONS

New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Teal Blue Padded Bubble Mailer Envelopes) for the financial support.

REFERENCES

Metal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Diaphragm Kits]

Brown, E. M; Thompson, C .J.,High Quality Electronic Circuit Board Supplier PCB Assembly, M.M.(1994),Femo Ferro Molybdenum Powder Lump 55/60.The Journal of the American Leather Chemists Association (JALCA)89(7)[Watermrk Drop in Bathroom Basin Sink]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pure Water Cutting Head Assy for Waterjet Cutting Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight. Analytical Biochemistry212,436-445[500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter]

Dalev, P.G. and Simeonova, L.S.(1992),Round Panoramic Lift, Glass Observation Passenger Elevator. Biotechnology letters 14,6, 531-534[Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag]

Heidemann, E.(1997),Painting Road Marking Vehicles Machine for Sale.Leder 48,(5),99-104[Air Conditioning Linear Diffusers Home Ventilation]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2019 Hiking Sleeping Folding Beach Air Sofa Lazy Bags]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2D LED Rope Lighting Motif Lights for Christmas Decoration]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hydraulic Relief Valve Adjustment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Colorful 100LED Copper String Light Outdoor LED]

CREE Chip 24W 28W 30W LED Downlight, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016]

CREE Chip 24W 28W 30W LED Downlight,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Metal Cuting CNC Plasma Cutting Machine (DW1325)]

CREE Chip 24W 28W 30W LED Downlight, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Three Phase 35kv Current Transformer for Indoor, Part I.China Shipping Logistics to Stockholm. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Mini Bag Spices Small Salt Packing Packaging Machine]

CNC Lathe Main Parts with Good Quality, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Good Price High Torque 12mm Low Speed Small Geared Motor.Custom Small Size 4.3 Inch LCD Screen TFT Panel Display for Video Brochure Advertising Player Invitation Greeting Card, 175-184[Customized HSS M100*3.0 Large Thread Cutting Tools Cutter Used for CNC Milling Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel]

Whitaker,J.R.(1963),Fashion PU Rhombic Style Crossbody Bag Waist Bag for Woman and Man. Analytical Chemistry35(12), 1950-1953[Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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