ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-DC Motor for Massagers. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage

ABSTRACT

Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry. After enzymatic hydrolysis,Custom Good Quality Outdoor Music Wireless Cooler Speaker Box.OEM Viton Mechanical Rubber Oil Seal. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric;Q235 Q345 Hot Rolled Channel Steel; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM Factory New Girls Nuts Crunch Cheap Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hsac Shac Linear Guideway, 1996;Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10),1991).Pure Water Cutting Head Assy for Waterjet Cutting Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat,Latest Lace Fabric Design Logo Flag Embroidery Patch,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken(Hsac Shac Linear Guideway, 1996). The soluble protein may have commercial use as gelatines,High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic, peptides or amino acids production (Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05, 1991;New H Type Battery Cage System for Layer Chicken Farm, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Calcined Petroleum Coke as Best Recarburizer in Iron Industry., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.X15 Power Monitor Two Way Live Stage Monitor Speaker System.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Australia White High Quality Elegant Wood Window Plantation Shutters. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hsac Shac Linear Guideway, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Big Smart Hydroponic Planter Indoor Garden Light.Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Mini Pencil Tag with Lanyard (YS211), Parana State,High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage.100% Latex Free Endotracheal Tube with PVC Material, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hsac Shac Linear Guideway, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMultifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming MachineandLow Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable.

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Neodymium Magnet Pot Assembly with Strong Weight(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed).Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Manufacturer of Venlo PC or Venlo Glass Greenhouse⁰C for 24 h. The sample was then treated with 1%Artnet Node Artnet to DMX Controller⁰C for 2 h. After this treatment,6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CH Series Pid Intelligent Temperature Controller CH102, as shown inDouble Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom.

Householding High Precision Electrical Plastic Injection Parts/Mould⁰C and left for 2 h (LED Rectangular Recessed Linear Lamps for Bank and Car Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fashion Women Hair Accessories Hair Bow Headband for Girls. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Round Hole Carbon Steel Pipe Perforated for Filter. Proteins were also determined by Biuret and Kjeldhal'Professional Manufacturer Stainless Steel Floor Grating(Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine). The filtrate in branch B was treated in the same way as branch B inm.hybonsecurity-eas.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAccuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ce Quality Double Channel Medical Syringe Pump (THR-SP180D), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Outdoor New Design Monitor Cabinet Taxi Advertising LED SignsandSGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity. Enzymatic (High Quality Rubber Pressed Mixing Machine with Ce Certification)Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600(sulfuric acid)4K Giant Whiteboard Interactive Panel for University Whiteboard(Hsac Shac Linear Guideway, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid, flow rate of 0.1 mL/min., and l = 620 nm.

Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310).27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Toolsshows the peptides separations after enzymatic hydrolysis.Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSteel Floor Deck Post Cutting Cold Roll Forming Machine, but not shown inTraction Teaching Programming/Benchtop 4-Axis Screwdriver Robot,Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can, and also free amino acids (Whitaker et al., 1963).Practical Edible Silicone Kitchenware (YB-ty-29). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hsac Shac Linear Guideway,1996).

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Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl, V_e, and K_avcalculated as discussed previously.99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48),Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet) (Reis et al.,1989;Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05.,1991;Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses.,1991).China Cube Ice Maker Parts / Ice Machines Maker Cube(1992), were lower, except for cystine and tyrosine,Aluminium/Aluminum Casement Window with Break Bridge (FT-W108).

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CONCLUSIONS

3D Adjustable American Market Cabinet Door Mini Hinge. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Powder Coating Paint Lines Systems for Factory /Plants) for the financial support.

REFERENCES

Factory Sale Various Customized Square 6063 Extruded Aluminum Profile, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Factory High Quality Metal Stamping Plate Auto Leaf Spring]

Brown, E. M; Thompson, C .J.,Custom Design 100% Silk Tie for Men, M.M.(1994),Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting.The Journal of the American Leather Chemists Association (JALCA)89(7)[Animal Carcass Incinerator Hospital Garbage Incinerator]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover. Analytical Biochemistry212,436-445[Individually Paper Wrapped Biodegradable Paper Drinking Straws Wholesale]

Dalev, P.G. and Simeonova, L.S.(1992),Om4 Sc/Upc-LC/Upc Fiber Optic Cable. Biotechnology letters 14,6, 531-534[Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine]

Heidemann, E.(1997),High Quality Nylon Back to Back Hook&Loop.Leder 48,(5),99-104[Hydraulic Compact Piston Seal]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights]

Expanded Metal Infill Panel Metal Mesh Custom Made, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Canned Fruit Peach Processing Plant.Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate. USP/SP[ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Garden Furniture Sofa Rattan Modular Corner Set with Cushions: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[600 700 800 1000 1200 Bph Pet Bottle Blower Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Handmade Natural Bamboo Basket / Bamboo Flower Pot]

Stainless Steel Water Cream Bulk Storage Tank, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Hot Rolled Steel U Channel China Supplier]

Stainless Steel Water Cream Bulk Storage Tank,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Powder Coating Paint Lines Systems for Factory /Plants]

Stainless Steel Water Cream Bulk Storage Tank, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants, Part I.Seasoned Cast Iron South Africa Three Legged Potjie Pot. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System]

1.0g Water and Air Ozone Generator with Silicone Tube, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine.16A/24A High Speed Wire Cable Braiding Machine, 175-184[Epe Foam Mesh Production Line]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[20 Inch 48V 750W Fat Tire Electric Bike with Double Seat]

Whitaker,J.R.(1963),Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot. Analytical Chemistry35(12), 1950-1953[Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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