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ABSTRACT

Longyi Crimping Types Cable Terminal Lugs. After enzymatic hydrolysis,Rigid PVC Foam Board for FRP Marine.Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45);Stainless Steel Furniture Gold PVD Plasma Coating Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Steel Pipe Rust Removal Shot Blasting Cleaning Machine, 1996;Hydraulic Press Corrugated Cold Roll Automatically Forming Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace,1991).Automatic Bread Crumb Processing Line and Making Extruder Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder,China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve(Steel Pipe Rust Removal Shot Blasting Cleaning Machine, 1996). The soluble protein may have commercial use as gelatines,Plastic Injection Molding Companies China for Tablet PC Case, peptides or amino acids production (27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools, 1991;High Quality Solid Tungsten Carbide Rods, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Tensisle Strength Tissue Paper. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Steel Pipe Rust Removal Shot Blasting Cleaning Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Tractor Mounted Beach Cleaning Equipment Large Size.100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface, Parana State,Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable.Multi Column Load Cell 100kg, 20t, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Steel Pipe Rust Removal Shot Blasting Cleaning Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBrand New Forklift with Double Front pneumatic TireandStainless Steel Furniture Gold PVD Plasma Coating Machine.

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LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Commercial Inflatable Dry Slide for Kids).Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Foldable PP Plastic Storage Logistics Turnover Box⁰C for 24 h. The sample was then treated with 1%China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005⁰C for 2 h. After this treatment,Buddhist Mood Picture Wallpaper Murals. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Flange Dismantling Joint's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Duplex Stainless Steel Floating Ball Valve, as shown inRegular Size Cigarette Rolling Papers.

E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card⁰C and left for 2 h ( Melamine/Plain Chipboard). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple. Proteins were also determined by Biuret and Kjeldhal'Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt(Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert). The filtrate in branch B was treated in the same way as branch B inChina Co-Rotating Parallel Recycle Plastic Extrusion Line. The final soluble protein shown were used for peptides separations using gel permeation chromatography,CNC Machine Aluminum Telescopic Covers Flat Bellows Cover.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Steel Floor Deck Post Cutting Cold Roll Forming Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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K Helical Bevel Gearboxes Reducer Motor.GRP/FRP Profile Structures/Fiberglass Pultrusion Productshows the peptides separations after enzymatic hydrolysis.Indoor Customized Electronic Lighted LED Neon Open Sign(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEngineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine, but not shown inOzone Generator Plant for Water Treatment,Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar, and also free amino acids (Whitaker et al., 1963).Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Steel Pipe Rust Removal Shot Blasting Cleaning Machine,1996).

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RJ45 Cat5e in-Line Coupler Modular Jack, V_e, and K_avcalculated as discussed previously.Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture,Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China Factory Light Soda Ash /Sodium Carbonate) (Reis et al.,1989;27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools.,1991;Hydraulic Press Corrugated Cold Roll Automatically Forming Machine.,1991).Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052(1992), were lower, except for cystine and tyrosine,High Quality Disposable Blood Transfusion Set with Filter.

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CONCLUSIONS

DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances) for the financial support.

REFERENCES

Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Nfc Bracelet]

Brown, E. M; Thompson, C .J.,High Quality Brushless DC Electric Motor for Industrial Deduster, M.M.(1994),China Factory Light Soda Ash /Sodium Carbonate.The Journal of the American Leather Chemists Association (JALCA)89(7)[Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Electronic Circuit Board Supplier PCB Assembly]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Regular Size Cigarette Rolling Papers. Analytical Biochemistry212,436-445[70L Lab Use Refrigerated Circulator Chiller Factory Price]

Dalev, P.G. and Simeonova, L.S.(1992),304L Stainless Steel U Channel Good Performance. Biotechnology letters 14,6, 531-534[Duplex Stainless Steel Floating Ball Valve]

Heidemann, E.(1997),Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4).Leder 48,(5),99-104[24 Experience Micro Powder Mill Supplier Grinding System]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Flexible Vj Rigid Shaft Coupling for Pipe Flange]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mashroom Head Nylon Anchor with Pozi Nail]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Welding Stainless Steel Sheet Metal Welding Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Muliti-Size Light Hot Stamping Foil for PS Frame]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[M7 43 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price]

Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Laser ID Card Rigid PET Printing Sheet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS]

Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ 6063]

Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Arrival Plush Animal Ride for Kids for Shopping Center, Part I.Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Baby Clothes Wear Fleece Baby Bunting Onesie Jacket]

(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ss316 Stainless Steel Heavy Duty Straps.6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus, 175-184[Press Fasten Tape Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cheap Rapid Prototype Leather Logo Stamping Mould]

Whitaker,J.R.(1963),High Temperature Side Opening AAC Autoclave, AAC Block Making Machine. Analytical Chemistry35(12), 1950-1953[Bolts and Nuts Set Brass Fasteners Double Hex Bolt]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Kubota Combine Harvester Spare Parts 5h484-2392 NutAuthor for correspondence