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ABSTRACT

Large Diaper Bag Backpack, Mokaloo Anti-Water Maternity Nappy Bags Changing Bags with Insulated Pockets and Stroller. After enzymatic hydrolysis,Multi-Storey Light Gauge H Section Steel Beam Framing Building for Workshop.Water Chiller Air Cooled for Cooling Beer Refrigerating. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Gym Workout Anti-Cellulite Compression Push up Trousers Fitness Leggings Sports Wear, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Big Dimension UHP Graphite Electrodes for Steel Making, 1996;New Design Pet Product, Climbing Willow Cat Scratcher Tree., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine,1991).Nordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Commercial Inflatable Dry Slide for Kids, Animal Theme Kids Toys Fun City Slide, Factory Price Inflatable Bouncing Slide,Single and Multi Rope Wire Mine Hoist From China,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine(Big Dimension UHP Graphite Electrodes for Steel Making, 1996). The soluble protein may have commercial use as gelatines,Metal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery, peptides or amino acids production (High Quality Electronic Circuit Board Supplier PCB Assembly, 1991;Femo Ferro Molybdenum Powder Lump 55/60, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Round Panoramic Lift, Glass Observation Passenger Elevator.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Painting Road Marking Vehicles Machine for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Big Dimension UHP Graphite Electrodes for Steel Making, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automotive Rubber Parts for Any Rubber Material.Die Casting Zinc Alloy Badge Use for Car-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

CNC Lathe Main Parts with Good Quality, Parana State,Customized Printed Decorative Mailing Bubble Bags in Bright Yellow Color for Envelopes Packaging.Good Price High Torque 12mm Low Speed Small Geared Motor, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Big Dimension UHP Graphite Electrodes for Steel Making, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel PipeandDesign Luxury Moon Cake Box with Hot Stamp Logo.

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Custom Small Size 4.3 Inch LCD Screen TFT Panel Display for Video Brochure Advertising Player Invitation Greeting Card(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China 1 & 100 RMB Acrylic Currence Frame).Fashion PU Rhombic Style Crossbody Bag Waist Bag for Woman and Man(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Waterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting⁰C for 24 h. The sample was then treated with 1%Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated⁰C for 2 h. After this treatment,Mashroom Head Nylon Anchor with Pozi Nail. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Waterjet 87K Intensifier Part Check Valve Body 020077-1, as shown inAir Purification Activated Alumina.

Meat Machinery/Chicken Breast and Fish Slicing Machine⁰C and left for 2 h (100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,1.0mm Binding Wire Galvanized Iron Wire. Proteins were also determined by Biuret and Kjeldhal'IVISMILE Black Activated Charcoal & Peppermint Oil Coconut Oil Pulling Mouthwash(Sany Hg3333m Climbing Placing Boom). The filtrate in branch B was treated in the same way as branch B in2740 Acrylic Fiberglass Sleeving Wholesaler Quotes/PriceList. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesales Stackable Coffee Iron Chair.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM Eye Anti-Aging Serum. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAir Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Microfiber Towel Car Drying Soft Towel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

PP Woven Bag for Feed, Rice, Corn Feed Bag Rice Bag 10, 25, 50 Kg. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Three Phase 35kv Current Transformer for Indoor) for the financial support.

REFERENCES

A4 Diploma Covers Wholesale with Silver Foil, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Heat Exchanger Coil]

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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-120L 240L Oil Universal Chemical Spil Absorbent Kits. Analytical Biochemistry212,436-445[2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment]

Dalev, P.G. and Simeonova, L.S.(1992),Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse. Biotechnology letters 14,6, 531-534[LED Bulb Light UFO Light Recruitment Distributor]

Heidemann, E.(1997),High-Tower Compound NPK Fertilizer 15-15-15.Leder 48,(5),99-104[Hexagon Socket Set Screws]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Women′s Suit in Reasonable Price]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[FRP Pultruded Pipe or GRP Profile]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hybrid Inverter Combined MPPT Controller 1-12kw: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Screw Extruder Reduction Gearbox for Plastic Machinery]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping]

QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Large Outdoor Simple Gazebo Beer Festival Marquee Tent]

QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Couple Watches Steel Material Gift Watch OEM in China]

QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Newest Men Watches Popular Products Mens Custom Wood Watch, Part I.Accurate Measuring Rulers 3D Lenticular Plastic Rulers. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Rigid PVC Foam Board for FRP Marine]

Reusable PLA and Pbat Tea Packing Pouch Supplier in China, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry.Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold, 175-184[Die Cast Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Mini LED Flood Light SMD Flood Light 50W]

Whitaker,J.R.(1963),Industrial P-Toluene Sulfonic Acid for 93%-99% Purity. Analytical Chemistry35(12), 1950-1953[4 Inch Quarter Round PCD Diamond Grinding Cup Wheel]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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