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www.emdachina.comEdivaldo E.Garcia^I; Cláudia C.M.UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Pressure Braided Rubber Oil Hose. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,High Quality Beech Wood Chiavari Chair at a Competitive

ABSTRACT

Gradient Print Overlay Push Button Membrane Keypad Switch. After enzymatic hydrolysis,Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X.Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Low Power Consumption Silicone Sex Doll Vending Machine, 1996;Toughened Glass Disc Suspension Insulator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller,1991).63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized Round Airless Lotion Pump Bottles for Skin Care Products,Round Shape Dinner Football Gift Bowl with Cover Plate,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Professional Exporter of Wheat Flour Mill Plant (80t)(Low Power Consumption Silicone Sex Doll Vending Machine, 1996). The soluble protein may have commercial use as gelatines,ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube, peptides or amino acids production (Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine, 1991;Aluminum Alloy Rack for Conductor, Wire, Cable, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Outdoor Accessories Fishing Hunting Masks., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-KERONG Security Safe Combination Smart Key Card Cabinet Door Lock. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Low Power Consumption Silicone Sex Doll Vending Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cast Floating Ball Valve China Manufacturer (GAQ41F).PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Main Pipe Tee Joint for Railway Piping System(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Single Core Solid Copper Conductor Rg Coaxial Cable, Parana State,High Quality Beech Wood Chiavari Chair at a Competitive.Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Low Power Consumption Silicone Sex Doll Vending Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wflandPVDF Exterior Design Curved Aluminum Panel.

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Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle).Baby Clothes Wear Fleece Baby Bunting Onesie Jacket(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Life Size 170cm Silicone Sex Dolls Real Love Doll for Male⁰C for 24 h. The sample was then treated with 1%Reflective Sheeting Material for Licence Plate⁰C for 2 h. After this treatment,Industrial Grade Nitrous Oxide for Hot Sales. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap, as shown inJy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs.

Fire Alarm Detector 24VDC Reflective Beam Detector⁰C and left for 2 h (Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Automatic Mini Bag Spices Small Salt Packing Packaging Machine. Proteins were also determined by Biuret and Kjeldhal'No 55 Steel Wire Cross Type Razor Barbed Wire(PP Coated Pipe Machine Extruder Machine). The filtrate in branch B was treated in the same way as branch B inExpansion Bolt. The final soluble protein shown were used for peptides separations using gel permeation chromatography,4V330-10 Air Compressor Solenoid Industrial Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Professional Various Standard Hot Sale Rotary Lab Single Round Punch. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMetal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Plastic Packaging Tray Vacuum Forming Machine.Medical Autoclavable Surgical Thoracoscopy Trocarshows the peptides separations after enzymatic hydrolysis.Permanent Suspended Magnetic Separator for Belt Conveyor(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Temperature Side Opening AAC Autoclave, but not shown inGreen Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber,Double Sides Clear White Frosted Acrylic Sheet, and also free amino acids (Whitaker et al., 1963).5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Low Power Consumption Silicone Sex Doll Vending Machine,1996).

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Good Price Channel Steel Used for Machinery, V_e, and K_avcalculated as discussed previously.Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator,Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit) (Reis et al.,1989;Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine.,1991;Toughened Glass Disc Suspension Insulator.,1991).Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount(1992), were lower, except for cystine and tyrosine,Factory Direct Sale Patent up to Down Rubber Tile Curing Press.

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CONCLUSIONS

Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b) for the financial support.

REFERENCES

Real Leather Wallet Mens Designer Leather Wallets, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automatic Shoe Polishing Machine]

Brown, E. M; Thompson, C .J.,Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338), M.M.(1994),Full Port Small Automatic Electronic Plumbing Ball Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[Plastic Material Handling Dolly Platform Hand Trolley Truck]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[500ml Blue Glass Beer Bottle with Crown Cap]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,. Analytical Biochemistry212,436-445[LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip]

Dalev, P.G. and Simeonova, L.S.(1992),Cleanroom White PVC Dotted Anti-Static Gloves. Biotechnology letters 14,6, 531-534[Chromium Oxide Green 99% for Construction and Plastics]

Heidemann, E.(1997),High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail.Leder 48,(5),99-104[Collar Earphones]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fashion Men′s Large Dial Casual Quartz Watch]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Elastic Hair Ties]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo]

ASTM A240 304 316L No. 1 Stainless Steel Sheet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),12V 24V 220V Digital Temperature Controller-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair]

ASTM A240 304 316L No. 1 Stainless Steel Sheet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ etc Underground Metel Detector]

ASTM A240 304 316L No. 1 Stainless Steel Sheet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price, Part I.25kg Fertilizer Belt Packing Machine for Special Materials. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove]

Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Rotor High Output Electric 120 Watt Motor.Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby, 175-184[(Midtech Industry) Plastic HDPE/PE Ocean Marine Pedal Profile Board Extruding Equipment Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Laser Cut Sheet Metal Fiber Laser Cutting Machine]

Whitaker,J.R.(1963),Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed. Analytical Chemistry35(12), 1950-1953[Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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