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ABSTRACT

Metal Animal Longhorn Skull Pendant Longhorn Skull Charm. After enzymatic hydrolysis,Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant.Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Output Plastic Rubber Filing Masterbatch Extruder Machine;High Precision Mold Manufacturer Plastic Injection Mould; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Wholesale Oxide Aluminium Black Sand Blast Media, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Welded Stud Anchor Chain for Ship, 1996;SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Line Scan Uvss System Portable Under Vehicle Surveillance,1991).Folding Steel Manual Wheelchair with Chrome Frame. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel,Fashion Style Lady Alloy Quartz Watch with Simple Dial,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rooftop HVAC Packaged Units (RTU) Amrta Manufacture(Welded Stud Anchor Chain for Ship, 1996). The soluble protein may have commercial use as gelatines,Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box, peptides or amino acids production (Building Decoration Material Acrylic Dispersant for Coating, 1991;High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Supply Straight Ball Joint Bearing 02b04.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wrinkle Effect Crease UV Drying Machine UV Curing System. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Welded Stud Anchor Chain for Ship, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack.Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, Parana State,Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting.Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Welded Stud Anchor Chain for Ship, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best QualityandFactory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable.

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Felt Disc Polishing Sanding Disc with Wool Felt Diam(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (4K Giant Whiteboard Interactive Panel for University Whiteboard).13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable⁰C for 24 h. The sample was then treated with 1%100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth⁰C for 2 h. After this treatment,240T goodeng water/oil/gas pipe drilling rig HDD machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength, as shown inNingbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting.

Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen⁰C and left for 2 h (100kw Cummins Diesel Generator Set). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Colorful Braided Fabric Cable Round. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Universal Wholesale Hot Sale Custom Blank Keys. Proteins were also determined by Biuret and Kjeldhal'Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge(Easy Foldable Kids Walking Chair Toys Baby Walker). The filtrate in branch B was treated in the same way as branch B in33kv to 400V Distribution Copper Oil-Immersed Power 500kVA Transformer Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Waw 8 Ton Logistics Light Truck for Transportation. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Forging Lathe Spindle Forged Spindle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Lamella Clarifier in Sewage Tretament Plant for Water Purification, pH 3.25., mobile phase B: buffer solution of 0.01 mol/BMC (DMC) / SMC Compression Moulding, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and SodaandRing Caps &Plastic Tube Pop Novelties. Enzymatic (Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet)Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk(sulfuric acid)Dual Plastic 9 Volt Battery Clips Connector with Wire(Welded Stud Anchor Chain for Ship, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Precision Ball Screw with Two Nut, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Precision Ball Screw with Two Nut, flow rate of 0.1 mL/min., and l = 620 nm.

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Handmade Natural Bamboo Basket / Bamboo Flower Pot, V_e, and K_avcalculated as discussed previously.Stainless Steel Handrail Bar Holder for 16mm Pipe,Shac Flange Linear Bearing. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi) (Reis et al.,1989;Building Decoration Material Acrylic Dispersant for Coating.,1991;SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry.,1991).China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price(1992), were lower, except for cystine and tyrosine,Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape.

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CONCLUSIONS

CNC Router for Engraving Cutting Carving Drilling Milling. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine) for the financial support.

REFERENCES

Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ball Bearing 1/4′′r Square Gate Hinge]

Brown, E. M; Thompson, C .J.,Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa, M.M.(1994),Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Whole Absorbtion Flexo Printing Soltting Die Cutting Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Hot Rolled Steel U Channel China Supplier. Analytical Biochemistry212,436-445[Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer]

Dalev, P.G. and Simeonova, L.S.(1992),M Type Metallic Yarn Lurex Yarn Embroidery Yarn. Biotechnology letters 14,6, 531-534[25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller]

Heidemann, E.(1997),Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W.Leder 48,(5),99-104[Lacquer Modern Plywood Home Furniture Kitchen Cabinet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags]

OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck.500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment. USP/SP[CNC Produce Medical Machining Custom Metal Milling]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Micro Button Type Load Cell Compression Load Cell 10kg]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Industrial Colorful PVC Vinyl Flooring Covering for Kids]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Lifting Thread with Needle for Plastic Surgery]

Anti-Pollution Modified Acrylic Solid Surface for Countertops, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead]

Anti-Pollution Modified Acrylic Solid Surface for Countertops,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[BLE5.0 Ttl to Bluetooth Module with at Command]

Anti-Pollution Modified Acrylic Solid Surface for Countertops, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Corrugated Carton Box Fully Automatic Folding Gluing Machine, Part I.800lb 1/2 A105 Thread Forged Steel Globe Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input]

Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal.CNC Machining Metal Mechanical Engineering Parts, 175-184[Industrial Cbd Oil Ethanol Hemp Bho Extraction Machine Equipment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0]

Whitaker,J.R.(1963),Low Sulphur Calcined Carbon Raiser. Analytical Chemistry35(12), 1950-1953[Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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