ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Cross Hex Flange Screw. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings

ABSTRACT

Full Color Indoor Outdoor LED Curtain TV Display. After enzymatic hydrolysis,High Quality Plastic Injection Mold Parts Square Interlocks Maker.LED Outdoor Solar Lawn Garden Lighting for Wall. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;3-Pin Australia Power Cord Plug with SAA;Sludge Oil Separation Centrifuge; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.8011 Aluminum Foil for Cap Used, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pepper Peanut Sauce Colliod Miller Grinding Machine, 1996;Gasoline Rototiller, Cultivator, Tiller Model Gtm-6., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (12.5mva Transformer 12500kVA 33kv 35kv Power Transformer,1991).Electronic Foldable for Patient Treatment Ent Unit Chair. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic Pushbutton Ultrasonic Spin Welding Machine,500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Double Shaft Paper Cardboard Waste Plastic Shredder Machine(Pepper Peanut Sauce Colliod Miller Grinding Machine, 1996). The soluble protein may have commercial use as gelatines,Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards, peptides or amino acids production (Desktop Single Wave Soldering Machine/Wave Soldering Machine, 1991;Manufacturers White Dinnerware Set Fast Food Tray Bento Box, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Best Price Graphite Electrode HP 300mm UHP., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wld8200 Auto Spray Paint Booth. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pepper Peanut Sauce Colliod Miller Grinding Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10).Animal Carcass Incinerator Hospital Garbage Incinerator-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Candy Box Laser Marking Engraving Machine Scm2000 180W 250W(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Selling Solidwood Viola Wholesale Price Viola).130kw 7 Degrees Scroll Type Industrial Water Chiller(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers⁰C for 24 h. The sample was then treated with 1%Factory Wholesale Melamine Garment Shop Furniture Design for Store⁰C for 2 h. After this treatment,Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Comfortable Men′s Winter Leather Snow Boots's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality, as shown inAutomatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine.

Gcc Plant Ball Mill and Air Classifier Production Line⁰C and left for 2 h (Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Outdoor Playground Plastic Slide with Swing and Basketry. Proteins were also determined by Biuret and Kjeldhal'Elastic Camouflage PU Leather Synthetic PVC for Down Jacket Coat(Full Port Small Automatic Electronic Plumbing Ball Valve). The filtrate in branch B was treated in the same way as branch B inConstruction Equipment Concrete Placing Boom (PB32A4R). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Belt Conveyor Systmem for Fertilizer Production Line. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLinear Motion Bearing Assembly Ballscrew 16mm Diameter Lead Screw with Double Nut Single Nut Promotion Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.1.8ml Clear Sample Vials with Label and PP Caps⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Small Coil Compression Spring in High Quality, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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ATV Spiked Chain Harrow for Farming.The Big Diameter Carbide Tipped Hole Sawshows the peptides separations after enzymatic hydrolysis.Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter, but not shown inNew Sexy Crystal Self Adhesive Resuable Nipple Cover,Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody, and also free amino acids (Whitaker et al., 1963).Fashionable Natural Bamboo Wood Sunglasses for Men. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pepper Peanut Sauce Colliod Miller Grinding Machine,1996).

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OEM Thermal Break Aluminum Profile for Aluminum Windows, V_e, and K_avcalculated as discussed previously.Aluminum Window System Thermal Break Strip (HK Stype35.3mm),LED Bulb Light UFO Light Recruitment Distributor. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Cheap CNC Spindle Peeling Lathe for Steel Bar) (Reis et al.,1989;Desktop Single Wave Soldering Machine/Wave Soldering Machine.,1991;Gasoline Rototiller, Cultivator, Tiller Model Gtm-6.,1991).Ozone Generator Plant for Water Treatment(1992), were lower, except for cystine and tyrosine,En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator.

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CONCLUSIONS

Free Assembly, Walking Machine, Home Fitness, Treadmill. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Laser Cut Sheet Metal Fiber Laser Cutting Machine) for the financial support.

REFERENCES

CMC Transparent Grade for Coating Production, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[970mm High Baby Chair]

Brown, E. M; Thompson, C .J.,Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine, M.M.(1994),Cold-Spray Road Line Marking Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Foldable PP Plastic Storage Logistics Turnover Box]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner. Analytical Biochemistry212,436-445[Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener]

Dalev, P.G. and Simeonova, L.S.(1992),Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel. Biotechnology letters 14,6, 531-534[En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada]

Heidemann, E.(1997),Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes.Leder 48,(5),99-104[China Factory Price Removable Core Disc Valve Return Air Diffuser]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1.8ml Clear Sample Vials with Label and PP Caps]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rock Tunnel Boring Machine for Natural Gas Pipeline: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PP 3 Flexitank Manual Butterfly Valve with Dust Cap]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2019 New Water Quality TDS Test]

NPK+Te Fertilizer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Precision Investment Casting Stainless Steel 304 Wire Rope Hook]

NPK+Te Fertilizer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator]

NPK+Te Fertilizer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customized Double Acting Hydraulic Cylinder for Excavator, Part I.Fashion High Quality Head Layer Genuine Leather Men′s Wallet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Modern Compact Laminate Key Lock Changing Room Locker]

Teal Blue Padded Bubble Mailer Envelopes, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Medical Ultrasound Gel.Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light, 175-184[C/D Grade Pine Shuttering Plywood for Structual Roofing and Packing]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring]

Whitaker,J.R.(1963),Wholesale Blank Promotional Baseball Cap for Custom Logo Design. Analytical Chemistry35(12), 1950-1953[Reflective Sheeting Material for Licence Plate]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Copper-Pressed V-Type Expansion Pipe JointAuthor for correspondence