ARTICLES

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ABSTRACT

Deep Hole Cylinder Boring Machine for Sale. After enzymatic hydrolysis,Hot Selling 202 Stainless Steel Coil.NPK 16X Hammer Spare Parts Racing Forged Piston. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve;Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mobile Hydraulic Concrete Placing Boom, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700, 1996;Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cr1000 Spray Nozzle Common Rail Injector Tester,1991).Industrial Cast Steel Flanged One Way Swing Check Valve Nrv. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Black White Stone 925 Silver Cross Pendants Necklace Jewelry,Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Kt Board Acrylic CCD Camera CNC Knife Cutting Machine(Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700, 1996). The soluble protein may have commercial use as gelatines,High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17, peptides or amino acids production (Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced, 1991;Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz.Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Carbon Steel Stainless Steel Countersunk Head Hollow Bolt(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Clear PVC Roof Frame Tent in South Africa, Parana State,Jc680 High Temperature Pressure Transmitter.High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the40X-1600X Biological Microscope Xsz-107V LED Lightand19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS.

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Stainless Steel Bend Pipe for Industrial Use(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Tire Repair Strip).Ybz-E16f20 Vane Steering Hydraulic Pumps(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Good Quality Snow White Pumpkin Seeds for Sale⁰C for 24 h. The sample was then treated with 1%ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors⁰C for 2 h. After this treatment,Automotive Acrylic Wholesale Masking Tape Factory Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper, as shown in400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making.

Watermrk Drop in Bathroom Basin Sink⁰C and left for 2 h (Acid Water Pulse Output Liquid Orifice Type Flow Meter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.OEM ABS Industrial Plastic Sheet Injection Auto Part. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,15L Big Cooling Cylinder High Production Hard Ice Cream Machine. Proteins were also determined by Biuret and Kjeldhal'Flange Type Ball Float Water Tank Control Valve(Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane). The filtrate in branch B was treated in the same way as branch B inM23 Round Plug 9 12 Pin Male MID Fixed Signal Connector. The final soluble protein shown were used for peptides separations using gel permeation chromatography,925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustomized Outdoor Plastic Chrome Auto Logo Signs(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Soundproof Construction Foam PVC/WPC Board, pH 3.25., mobile phase B: buffer solution of 0.01 mol/UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Meat Machinery/Chicken Breast and Fish Slicing MachineandHigh Pressure Braided Rubber Oil Hose. Enzymatic (High Quality Beech Wood Chiavari Chair at a Competitive)Gradient Print Overlay Push Button Membrane Keypad Switch(sulfuric acid)Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X(Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004), flow rate of 0.1 mL/min., and l = 620 nm.

Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform.Aluminum Window System Thermal Break Strip (HK Stype35.3mm)shows the peptides separations after enzymatic hydrolysis.55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAuto Parts Cross Propeller Shaft for Mercedes Benz 12062X, but not shown inDie Casting Zinc Alloy Badge Use for Car,AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle, and also free amino acids (Whitaker et al., 1963).Low Power Consumption Silicone Sex Doll Vending Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700,1996).

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Customized Round Airless Lotion Pump Bottles for Skin Care Products, V_e, and K_avcalculated as discussed previously.Round Shape Dinner Football Gift Bowl with Cover Plate,Professional Exporter of Wheat Flour Mill Plant (80t). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304) (Reis et al.,1989;Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced.,1991;Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll.,1991).ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube(1992), were lower, except for cystine and tyrosine,Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine.

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CONCLUSIONS

Aluminum Alloy Rack for Conductor, Wire, Cable. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable) for the financial support.

REFERENCES

Outdoor Accessories Fishing Hunting Masks, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[12V 24V High Power Brushless DC Electric Motor for Pump]

Brown, E. M; Thompson, C .J.,Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box, M.M.(1994),KERONG Security Safe Combination Smart Key Card Cabinet Door Lock.The Journal of the American Leather Chemists Association (JALCA)89(7)[FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cast Floating Ball Valve China Manufacturer (GAQ41F). Analytical Biochemistry212,436-445[Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts]

Dalev, P.G. and Simeonova, L.S.(1992),PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda. Biotechnology letters 14,6, 531-534[OEM Eye Anti-Aging Serum]

Heidemann, E.(1997),Stainless Steel Main Pipe Tee Joint for Railway Piping System.Leder 48,(5),99-104[m.mjbagging.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[900-1800MHz GSM PCB Antenna]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Q-Sub Subwoofer Professional Passive Speaker Loud Speaker Woofer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ot/Breakbulk Container Service From China to South America]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Neodymium Magnet Pot Assembly with Strong Weight]

Full Automatic Paper Lunch Food Box Carton Forming Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Single Core Solid Copper Conductor Rg Coaxial Cable-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Clear PVC Roof Frame Tent in South Africa]

Full Automatic Paper Lunch Food Box Carton Forming Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Soundproof Construction Foam PVC/WPC Board]

Full Automatic Paper Lunch Food Box Carton Forming Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use, Part I.Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale CNC Router Machine for Wood Cutting (1325)]

Baby Clothes Wear Fleece Baby Bunting Onesie Jacket, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Life Size 170cm Silicone Sex Dolls Real Love Doll for Male.Reflective Sheeting Material for Licence Plate, 175-184[Single Billet Heating Aluminium Alloy Aging Furnace]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Customized Wire Harness for Various Application]

Whitaker,J.R.(1963),Industrial Grade Nitrous Oxide for Hot Sales. Analytical Chemistry35(12), 1950-1953[Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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