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ABSTRACT

Wood Fireplace Double Skin Chimney Flue Pipes. After enzymatic hydrolysis,High Quality 304 Stainless Steel Sheet for Home Decoration Material.T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;LED Rectangular Recessed Linear Lamps for Bank and Car Sale;Salon Equipment Hair Clipper; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Flat Conveyor Belt Steel Idler Frame for Roller, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6, 1996;Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Durable TPR Pet Molar Toy with Colorful Rope,1991).Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Chromium Oxide Green 99% for Construction and Plastics,Zs Round Vibrating Sieve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Tactile Paving Blind Road Stud(LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6, 1996). The soluble protein may have commercial use as gelatines,70L Lab Use Refrigerated Circulator Chiller Factory Price, peptides or amino acids production (Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500), 1991;Roller Hearth Heating Furnace for Gas Cylindrer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;custom design MIFARE DESFire EV2 2K plastic RFID loyalty card., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hotel Card Door Lock Access Control Safe Door Lock. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer.Stainless Steel DIN7981 Pan Head Self Tapping Screw-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib, Parana State,HOWO Tipper Truck Price with Manufacture Factory Price.Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality Disposable Blood Transfusion Set with FilterandCustom FSTN Va COB Cog Segment LCD Display Module.

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High Speed HDMI Cable Wholesale HDMI with Ethernet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine).Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Women′s Suit in Reasonable Price⁰C for 24 h. The sample was then treated with 1%Pet Short Fiber Non Woven Geotextile for Landfill Filtration⁰C for 2 h. After this treatment,Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Competitive Price Sodium Gluconate as Concrete Admixture's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Precision Investment Casting Stainless Steel 304 Wire Rope Hook, as shown inAutomatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator.

AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture⁰C and left for 2 h (Nbm High Quality Auto Part Factory Price 6pk2310). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom Die Casting Aluminium Product for Connector Usage. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel Balustrade/Pillar/Column. Proteins were also determined by Biuret and Kjeldhal'Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller(Swf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formations). The filtrate in branch B was treated in the same way as branch B inMicronized Sieve Machine Ultrasonic Vibrating Screen. The final soluble protein shown were used for peptides separations using gel permeation chromatography,PP/Pet Woven Geotextile for Road Construction.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Lamella Clarifier in Industrial Wastewater Treatment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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50g Body Luxury King Crown Cosmetic Containers.LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6shows the peptides separations after enzymatic hydrolysis.Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBiodegradable Food Packaging Take out Food Boxes, but not shown inTop Furniture Hotel Dining Furniture Metal Hotel Table Chair,Mini Succulent Planters, Cactus Pots with Wooden Cement Pot, and also free amino acids (Whitaker et al., 1963).Paper Egg Box Forming Molding Production Line/ Egg Tray Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6,1996).

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CONCLUSIONS

Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Csm DIY Microscope for Kids/Toy Microscope) for the financial support.

REFERENCES

Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Radiator Auto Fan Shroud Plastic Automotive Part Plastic Injection Molding Parts]

Brown, E. M; Thompson, C .J.,Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo, M.M.(1994),N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet.The Journal of the American Leather Chemists Association (JALCA)89(7)[Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste). Analytical Biochemistry212,436-445[Stainless Steel Handrail Bar Holder for 16mm Pipe]

Dalev, P.G. and Simeonova, L.S.(1992),Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale. Biotechnology letters 14,6, 531-534[6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China]

Heidemann, E.(1997),High Quaity China Supplier Truck Trailer Auto Car Accessories.Leder 48,(5),99-104[kids playground]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence]

16oz Insulated Cardboad Cup Sleeve, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable.Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light. USP/SP[Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Steel Investment Casting High Hardness Agricultural Machinery Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Outdoor Multifunctional Solar Power Flashlight (SYSG-517)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Shoulder Stability Brace with Pressure Ice Pad Made of Light and Breathable Neoprene]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cross Hex Flange Screw]

Minimalist Slim Real Leather Front Pocket Card Wallet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Natural Cleaning Konjak Sponge-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Small Coil Compression Spring in High Quality]

Minimalist Slim Real Leather Front Pocket Card Wallet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10)]

Minimalist Slim Real Leather Front Pocket Card Wallet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Indoor Load Break Switch Isolating Switch, Part I.Biodegradable Disposible Straw Pulp Food Box Ppp-1007. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Stainless Steel Mechanical Filter Hosing]

T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment.NBR Latex Adhesive for Glove, 175-184[Plastic Mould Dongguan Molding Factory]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel]

Whitaker,J.R.(1963),Cheap Wooden House Zinc Plated Angle Bracket. Analytical Chemistry35(12), 1950-1953[Professional Various Standard Hot Sale Rotary Lab Single Round Punch]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Poultry Farming Equipment Broiler Chicken Ground Floor Rearing SystemAuthor for correspondence