ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Building Decoration Material Acrylic Dispersant for Coating. After enzymatic hydrolysis,High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine.Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Factory Supply Straight Ball Joint Bearing 02b04;Wrinkle Effect Crease UV Drying Machine UV Curing System; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector, 1996;20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Tefude Feeding and Packaging Line for Food with Tray,1991).Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine,CMP No Nc Metal Switch Black 25mm Illuminated Push Button,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks(Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector, 1996). The soluble protein may have commercial use as gelatines,Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil, peptides or amino acids production (Felt Disc Polishing Sanding Disc with Wool Felt Diam, 1991;13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-240T goodeng water/oil/gas pipe drilling rig HDD machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas.Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Waw 8 Ton Logistics Light Truck for Transportation, Parana State,Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box.Hot Forging Lathe Spindle Forged Spindle, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMarine Solas Adhesive Reflective TapeandMould Lifecycle: We Think About The Entire Life of The Mould.We Consider The Finishing.

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Hot Sale Chic Straw Braid Floppy Hat with Print Tape(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (HPMC Similiar to Tylose Powder for Rapid-Set Adhesive).High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Lamella Clarifier in Sewage Tretament Plant for Water Purification⁰C for 24 h. The sample was then treated with 1%BMC (DMC) / SMC Compression Moulding⁰C for 2 h. After this treatment,Ring Caps &Plastic Tube Pop Novelties. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk, as shown inLiquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass.

Dual Plastic 9 Volt Battery Clips Connector with Wire⁰C and left for 2 h (Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Precision Ball Screw with Two Nut. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection. Proteins were also determined by Biuret and Kjeldhal'Low Density Cleaner in Paper Making Machine(Tk 4100). The filtrate in branch B was treated in the same way as branch B inBest Price Wireless RF 12V Wireless on/off Remote Control Switch Jh-Tx49. The final soluble protein shown were used for peptides separations using gel permeation chromatography,En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customized Stainless Steel Laser Cutting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPrice of a Retail Supermarket Push Cart for Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Handmade Natural Bamboo Basket / Bamboo Flower Pot, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Handrail Bar Holder for 16mm Pipe, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Industrial P-Toluene Sulfonic Acid for 93%-99% PurityandShac Flange Linear Bearing. Enzymatic (China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price)Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape(sulfuric acid)CNC Router for Engraving Cutting Carving Drilling Milling(Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa.Custom Grooved Quartz Plate Deep Processingshows the peptides separations after enzymatic hydrolysis.Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering, but not shown inCommercial Double Lane Inflatable Carnival Bungee Run Game,High Quality Hot Rolled Steel U Channel China Supplier, and also free amino acids (Whitaker et al., 1963).M Type Metallic Yarn Lurex Yarn Embroidery Yarn. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector,1996).

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YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable, V_e, and K_avcalculated as discussed previously.Anti-Pollution Modified Acrylic Solid Surface for Countertops,Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Professional Plastic Plating Makeup Brush Set) (Reis et al.,1989;Felt Disc Polishing Sanding Disc with Wool Felt Diam.,1991;20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory.,1991).Corrugated Carton Box Fully Automatic Folding Gluing Machine(1992), were lower, except for cystine and tyrosine,800lb 1/2 A105 Thread Forged Steel Globe Valve.

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CONCLUSIONS

Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge) for the financial support.

REFERENCES

China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[May Collapse Dyadic Interlayer Boiler]

Brown, E. M; Thompson, C .J.,CNC Machining Metal Mechanical Engineering Parts, M.M.(1994),Low Sulphur Calcined Carbon Raiser.The Journal of the American Leather Chemists Association (JALCA)89(7)[ASTM A240 304 316L No. 1 Stainless Steel Sheet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Selling Free Sample Natural Fragrant Bath SPA Salt. Analytical Biochemistry212,436-445[China Round Transparent Glass 750ml Liquor Glass Bottle]

Dalev, P.G. and Simeonova, L.S.(1992),Custom FSTN Va COB Cog Segment LCD Display Module. Biotechnology letters 14,6, 531-534[New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera]

Heidemann, E.(1997),Jc680 High Temperature Pressure Transmitter.Leder 48,(5),99-104[LCD Display Industrial Platform Scale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-NPK 16X Hammer Spare Parts Racing Forged Piston: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Na2co3 Soda Ash Dense]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel]

2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool]

2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera]

2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mobile Hydraulic Concrete Placing Boom, Part I.Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation]

Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cr1000 Spray Nozzle Common Rail Injector Tester.Industrial Cast Steel Flanged One Way Swing Check Valve Nrv, 175-184[Outdoor Travelling Large Compartment Insulated Trolley Picnic Cooler Bag with Wheels]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover]

Whitaker,J.R.(1963),Black White Stone 925 Silver Cross Pendants Necklace Jewelry. Analytical Chemistry35(12), 1950-1953[Round Modern Furniture Leisure Sofa for Promotion]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hot Selling 202 Stainless Steel CoilAuthor for correspondence