ARTICLES

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ABSTRACT

Automatic Bread Crumb Processing Line and Making Extruder Machine. After enzymatic hydrolysis,Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder.China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve;Plastic Injection Molding Companies China for Tablet PC Case; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Solid Tungsten Carbide Rods, 1996;Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved,1991).High Tensisle Strength Tissue Paper. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Tractor Mounted Beach Cleaning Equipment Large Size,100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts(High Quality Solid Tungsten Carbide Rods, 1996). The soluble protein may have commercial use as gelatines,PP Coated Pipe Machine Extruder Machine, peptides or amino acids production (China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts, 1991;Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mining Machinery Parts Diamond Saw Blade Segments for Marble., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Multi Column Load Cell 100kg, 20t. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Solid Tungsten Carbide Rods, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System.Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Foldable PP Plastic Storage Logistics Turnover Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card, Parana State,2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace.Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality Solid Tungsten Carbide Rods, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Sodaand2019 New Tuya Smart Wireless WiFi Smart Socket (PST-J2).

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Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Round Airless Lotion Pump Bottles for Skin Care Products).Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CNC Machine Aluminum Telescopic Covers Flat Bellows Cover⁰C for 24 h. The sample was then treated with 1%7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input⁰C for 2 h. After this treatment,Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Kubota Combine Harvester Spare Parts 5h484-2392 Nut's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable, as shown inCreative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass.

HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor⁰C and left for 2 h (ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Steel Floor Deck Post Cutting Cold Roll Forming Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180). Proteins were also determined by Biuret and Kjeldhal'Fluorous Rubber Coated Fiberglass Cloth(100% Latex Free Endotracheal Tube with PVC Material). The filtrate in branch B was treated in the same way as branch B inEducational Equipment Smart Multi Touch Interactive White Board for School. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPS Foam Disposable Fast Food Box Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Indoor Customized Electronic Lighted LED Neon Open Sign⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper.Latest Lace Fabric Design Logo Flag Embroidery Patchshows the peptides separations after enzymatic hydrolysis.Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi, but not shown inPlastic Waste Pet Flakes Recycling Washing Machine,High Quality Disposable Blood Transfusion Set with Filter, and also free amino acids (Whitaker et al., 1963).DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Solid Tungsten Carbide Rods,1996).

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Regular Size Cigarette Rolling Papers, V_e, and K_avcalculated as discussed previously.304L Stainless Steel U Channel Good Performance,Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags) (Reis et al.,1989;China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts.,1991;Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover.,1991).Junior Backyard Training Practise Soccer Goal Net(1992), were lower, except for cystine and tyrosine,350mm Diamond Blade for Granite.

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CONCLUSIONS

Custom Welding Stainless Steel Sheet Metal Welding Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Flange Dismantling Joint) for the financial support.

REFERENCES

Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Hammer Exhaust Fan for Agriculture/Greenhouse/Poutry Farms]

Brown, E. M; Thompson, C .J.,Laser ID Card Rigid PET Printing Sheet, M.M.(1994),New Arrival Plush Animal Ride for Kids for Shopping Center.The Journal of the American Leather Chemists Association (JALCA)89(7)[Micro Button Type Load Cell Compression Load Cell 10kg]

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Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine. Analytical Biochemistry212,436-445[Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead]

Dalev, P.G. and Simeonova, L.S.(1992),(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine. Biotechnology letters 14,6, 531-534[BLE5.0 Ttl to Bluetooth Module with at Command]

Heidemann, E.(1997),Ss316 Stainless Steel Heavy Duty Straps.Leder 48,(5),99-104[Auto Brake Pad]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input]

Steel Pipe Rust Removal Shot Blasting Cleaning Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus.High Temperature Side Opening AAC Autoclave, AAC Block Making Machine. USP/SP[Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[RJ45 Cat5e in-Line Coupler Modular Jack]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tangent Supports Suspension Clamp]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[1.0g Water and Air Ozone Generator with Silicone Tube]

Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Wholesale Oxide Aluminium Black Sand Blast Media]

Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror]

Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine, Part I.New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CNC Router for Engraving Cutting Carving Drilling Milling]

Green Color PVC Anti Dazzle Panel, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator.XL-21 Motive Electrical Switchgear Power Cabinet, 175-184[Fresh Mint Flavor Remove Stain Eco Friendly Natural Activated Bamboo Charcoal Toothpaste]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair]

Whitaker,J.R.(1963),Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter. Analytical Chemistry35(12), 1950-1953[Industrial PVC High Pressure Oxygen Acetylene Twin Welding Sealing Double-Color Gas Cutting Flexible Air Hose]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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