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ABSTRACT

High Quality Solid Tungsten Carbide Rods. After enzymatic hydrolysis,Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover.Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Tensisle Strength Tissue Paper;Tractor Mounted Beach Cleaning Equipment Large Size; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts, 1996;PP Coated Pipe Machine Extruder Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts,1991).Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mining Machinery Parts Diamond Saw Blade Segments for Marble,Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Multi Column Load Cell 100kg, 20t(Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts, 1996). The soluble protein may have commercial use as gelatines,LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System, peptides or amino acids production (Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck, 1991;Foldable PP Plastic Storage Logistics Turnover Box, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Buddhist Mood Picture Wallpaper Murals.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Flange Dismantling Joint. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Duplex Stainless Steel Floating Ball Valve.E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence, Parana State,27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools.Kubota Combine Harvester Spare Parts 5h484-2392 Nut, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSolar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4)andHigh-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine.

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Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Direct Sale Patent up to Down Rubber Tile Curing Press).HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Steel Floor Deck Post Cutting Cold Roll Forming Machine⁰C for 24 h. The sample was then treated with 1%Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180)⁰C for 2 h. After this treatment,Fluorous Rubber Coated Fiberglass Cloth. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern), as shown inImirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar.

PS Foam Disposable Fast Food Box Making Machine⁰C and left for 2 h (Steel Pipe Rust Removal Shot Blasting Cleaning Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.K Helical Bevel Gearboxes Reducer Motor. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Indoor Customized Electronic Lighted LED Neon Open Sign. Proteins were also determined by Biuret and Kjeldhal'Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar(Mining Machinery Parts Diamond Saw Blade Segments for Marble). The filtrate in branch B was treated in the same way as branch B in4K-90 Diesel Rice Wheat Mini Reaper Binder Harvester Machine Factory Supply. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Free Logo Design Cheap Hand Flag (NF01F02021). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.RJ45 Cat5e in-Line Coupler Modular Jack⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Buddhist Mood Picture Wallpaper MuralsandCold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052. Enzymatic (High Quality Disposable Blood Transfusion Set with Filter)DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle(sulfuric acid)Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194)(Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Brushless DC Electric Motor for Industrial Deduster, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Brushless DC Electric Motor for Industrial Deduster, flow rate of 0.1 mL/min., and l = 620 nm.

China Factory Light Soda Ash /Sodium Carbonate.Outdoor Garden Furniture Baby Plastic Swing for Saleshows the peptides separations after enzymatic hydrolysis.Regular Size Cigarette Rolling Papers(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inElectric Manual Ice Crusher Ice Shaver Industrial Ice Machine, but not shown ina PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine,304L Stainless Steel U Channel Good Performance, and also free amino acids (Whitaker et al., 1963).Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts,1996).

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CONCLUSIONS

Ss316 Stainless Steel Heavy Duty Straps. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Machine Aluminum Telescopic Covers Flat Bellows Cover) for the financial support.

REFERENCES

6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bluetooth Barcode Scanner Ios Android 1d Laser Scanner Module with Real Time Clock with High Resolution Ms3391-L]

Brown, E. M; Thompson, C .J.,High Temperature Side Opening AAC Autoclave, AAC Block Making Machine, M.M.(1994),PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[100kw Cummins Diesel Generator Set]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation. Analytical Biochemistry212,436-445[Easy Foldable Kids Walking Chair Toys Baby Walker]

Dalev, P.G. and Simeonova, L.S.(1992),Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal. Biotechnology letters 14,6, 531-534[PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda]

Heidemann, E.(1997),1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine.Leder 48,(5),99-104[PPGI PPGL China Coil Supplier with Good Price for Sheet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2019 New Tuya Smart Wireless WiFi Smart Socket (PST-J2)]

300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar.Green Color PVC Anti Dazzle Panel. USP/SP[High Quality Rubber Pressed Mixing Machine with Ce Certification]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Customized Round Airless Lotion Pump Bottles for Skin Care Products]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Coffee Mixer Tool]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors]

XL-21 Motive Electrical Switchgear Power Cabinet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[100% Latex Free Endotracheal Tube with PVC Material]

XL-21 Motive Electrical Switchgear Power Cabinet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Solar Powered Outdoor Lighting LED Solar Wall Light]

XL-21 Motive Electrical Switchgear Power Cabinet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Furniture Grade E1 Glue Plywood with Competitive Price, Part I.High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Latest Lace Fabric Design Logo Flag Embroidery Patch]

TUV Certificate High Quality Single Core PV1-F PV Solar Cables, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners.Cast Steel Dual Plate Wafer Check Valve, 175-184[Ductile Iron Sand Casting with ISO9001: 2008]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi]

Whitaker,J.R.(1963),Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products. Analytical Chemistry35(12), 1950-1953[Plastic Waste Pet Flakes Recycling Washing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

BeansAuthor for correspondence