ARTICLES

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ABSTRACT

Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG). After enzymatic hydrolysis,Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl.99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump;China Cube Ice Maker Parts / Ice Machines Maker Cube; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminium/Aluminum Casement Window with Break Bridge (FT-W108), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3D Adjustable American Market Cabinet Door Mini Hinge, 1996;Factory Sale Various Customized Square 6063 Extruded Aluminum Profile., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Design 100% Silk Tie for Men,1991).Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover,Om4 Sc/Upc-LC/Upc Fiber Optic Cable,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Nylon Back to Back Hook&Loop(3D Adjustable American Market Cabinet Door Mini Hinge, 1996). The soluble protein may have commercial use as gelatines,Canned Fruit Peach Processing Plant, peptides or amino acids production (Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, 1991;Garden Furniture Sofa Rattan Modular Corner Set with Cushions, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Water Cream Bulk Storage Tank., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3D Adjustable American Market Cabinet Door Mini Hinge, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Seasoned Cast Iron South Africa Three Legged Potjie Pot.1.0g Water and Air Ozone Generator with Silicone Tube-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Small Boat Deck Knuckle Boom Ship Pedestal Crane(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Indoor Load Break Switch Isolating Switch).115mm Segmented Saw Blade for Concrete Cutting(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit⁰C for 24 h. The sample was then treated with 1%China Round Transparent Glass 750ml Liquor Glass Bottle⁰C for 2 h. After this treatment,3D Creative Design Metal Medal Promotion Gift of Sport. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ad-L13 Black Perfume Plastics Vial Bottle 10ml's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover, as shown inSTPP Sodium Tripolyphosphate for Detergent / Ceramic / Food.

EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012)⁰C and left for 2 h (Nordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Universal Mirco Forklift Car Bus Truck Seats Switch Sensors. Proteins were also determined by Biuret and Kjeldhal'Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder(EPS PS Foam Food Container Making Machine). The filtrate in branch B was treated in the same way as branch B inBaby Expressions Postbound Album - Pink (8x8\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\") Acid Free Sheets With Pp Pocket. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Mexico Single Expansion Anchor with YZP.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Whole Absorbtion Flexo Printing Soltting Die Cutting Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Price 4X6 Acrylic Magnetic Photo Frame, pH 3.25., mobile phase B: buffer solution of 0.01 mol/200m Diesel Oil Hydraulic Water Well Drilling Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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FRP Pultruded Pipe or GRP Profile.Automotive Acrylic Wholesale Masking Tape Factory Priceshows the peptides separations after enzymatic hydrolysis.6090 CO2 Sealed Glass Cooling Laser Cutting Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting, but not shown in120ml Glass Perfume Bottles for Designer Perfume,Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator, and also free amino acids (Whitaker et al., 1963).Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3D Adjustable American Market Cabinet Door Mini Hinge,1996).

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CONCLUSIONS

High Output Plastic Rubber Filing Masterbatch Extruder Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050)) for the financial support.

REFERENCES

High Precision Mold Manufacturer Plastic Injection Mould, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[SMA Male Plug to N Male Cable Connector for Rg316D Cable Jumper Assembly]

Brown, E. M; Thompson, C .J.,China Wholesale Oxide Aluminium Black Sand Blast Media, M.M.(1994),Welded Stud Anchor Chain for Ship.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Supply Straight Ball Joint Bearing 02b04]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Customized Cute Animal Dinosaur Kids Baby Baseball Cap]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry. Analytical Biochemistry212,436-445[High-Tower Compound NPK Fertilizer 15-15-15]

Dalev, P.G. and Simeonova, L.S.(1992),Line Scan Uvss System Portable Under Vehicle Surveillance. Biotechnology letters 14,6, 531-534[4.0MP Ahd Tvi CVBS HD CCTV Camera Module]

Heidemann, E.(1997),Folding Steel Manual Wheelchair with Chrome Frame.Leder 48,(5),99-104[m.prestigelaminatefloor.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Precision Ball Screw with Two Nut]

Practical Edible Silicone Kitchenware (YB-ty-29), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel.Fashion Style Lady Alloy Quartz Watch with Simple Dial. USP/SP[Wood Fireplace Double Skin Chimney Flue Pipes]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rooftop HVAC Packaged Units (RTU) Amrta Manufacture: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Screw Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Zero Defect Carton/Box Automatic  Strapping Packaging Packing Pack Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide]

Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Building Decoration Material Acrylic Dispersant for Coating-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear]

Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen]

Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine, Part I.Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Agriculture Apple Orchard Reflective Pet Film]

Factory Supply Straight Ball Joint Bearing 02b04, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wrinkle Effect Crease UV Drying Machine UV Curing System.Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack, 175-184[Long Hair Swimming Cap]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Tct Circular Saw Blade &Diamond Saw Blade]

Whitaker,J.R.(1963),Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector. Analytical Chemistry35(12), 1950-1953[5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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