ARTICLES

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ABSTRACT

Stainless Steel Handrail Bar Holder for 16mm Pipe. After enzymatic hydrolysis,Shac Flange Linear Bearing.China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa, 1996;Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Hot Rolled Steel U Channel China Supplier,1991).M Type Metallic Yarn Lurex Yarn Embroidery Yarn. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W,Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment(Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa, 1996). The soluble protein may have commercial use as gelatines,YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable, peptides or amino acids production (Anti-Pollution Modified Acrylic Solid Surface for Countertops, 1991;Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Corrugated Carton Box Fully Automatic Folding Gluing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.800lb 1/2 A105 Thread Forged Steel Globe Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal.CNC Machining Metal Mechanical Engineering Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Low Sulphur Calcined Carbon Raiser(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hot Selling 202 Stainless Steel Coil, Parana State,Handmade Natural Bamboo Basket / Bamboo Flower Pot.NPK 16X Hammer Spare Parts Racing Forged Piston, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theProfessional Manufacturer Stainless Steel Floor GratingandSafety Full Automatic Electric Opening AAC Autoclave for AAC Plant.

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2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part).Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mobile Hydraulic Concrete Placing Boom⁰C for 24 h. The sample was then treated with 1%Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700⁰C for 2 h. After this treatment,Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cr1000 Spray Nozzle Common Rail Injector Tester's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Industrial Cast Steel Flanged One Way Swing Check Valve Nrv, as shown inPP/Pet Woven Geotextile for Road Construction.

Black White Stone 925 Silver Cross Pendants Necklace Jewelry⁰C and left for 2 h (Plastic Injection Molding Companies China for Tablet PC Case). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Kt Board Acrylic CCD Camera CNC Knife Cutting Machine. Proteins were also determined by Biuret and Kjeldhal'High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17(Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant). The filtrate in branch B was treated in the same way as branch B in3 Layers 3000L Plastic Blow Molding Machine/Blowing Moulding Machiery. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDrilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Flange Type Ball Float Water Tank Control Valve. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Jc680 High Temperature Pressure Transmitter) for the financial support.

REFERENCES

925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Large Scale 10mm Three Layer Hollow Polycarbonate Sheet Covered Greenhouse for Sale]

Brown, E. M; Thompson, C .J.,Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear, M.M.(1994),Customized Outdoor Plastic Chrome Auto Logo Signs.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Leaf Shape Pearl Jewelry Wedding Tiara]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Small Table CNC Marble Engraver for Stone. Analytical Biochemistry212,436-445[Cold Drawn D2 H13 Alloy Steel Turning Roll]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4. Biotechnology letters 14,6, 531-534[ Metal Polishing Disc]

Heidemann, E.(1997),China Soundproof Construction Foam PVC/WPC Board.Leder 48,(5),99-104[High Quality & Best Price Manufacturer Wood Log Debarker]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Container House for Clinic or Hospital]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Beech Wood Chiavari Chair at a Competitive: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Customized Stamping/Punch-Tooling-Sheet Metal Pressing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Competitive Price Sodium Gluconate as Concrete Admixture]

Gradient Print Overlay Push Button Membrane Keypad Switch, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cr1000 Spray Nozzle Common Rail Injector Tester]

Gradient Print Overlay Push Button Membrane Keypad Switch,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[24 Hours Self-Service Water ATM Machine Automatic RO Water Vending Machine]

Gradient Print Overlay Push Button Membrane Keypad Switch, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004), Part I.Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple]

55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle.Low Power Consumption Silicone Sex Doll Vending Machine, 175-184[Drop Cable Fiber Optic]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom Good Quality Outdoor Music Wireless Cooler Speaker Box]

Whitaker,J.R.(1963),Toughened Glass Disc Suspension Insulator. Analytical Chemistry35(12), 1950-1953[ Marble Child Statue for Garden]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Motorcycle Tube Making Machine/Inner Tube Making MachineAuthor for correspondence