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ABSTRACT

PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag. After enzymatic hydrolysis,Aluminum Hot Forging Accessories Machining Parts.Building Wall Machine for Hot Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Expanded Metal Infill Panel Metal Mesh Custom Made, 1996;DC Motor for Massagers., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage,1991).Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Good Quality Outdoor Music Wireless Cooler Speaker Box,OEM Viton Mechanical Rubber Oil Seal,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric(Expanded Metal Infill Panel Metal Mesh Custom Made, 1996). The soluble protein may have commercial use as gelatines,Q235 Q345 Hot Rolled Channel Steel, peptides or amino acids production (OEM Factory New Girls Nuts Crunch Cheap Price, 1991;Hsac Shac Linear Guideway, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pure Water Cutting Head Assy for Waterjet Cutting Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Expanded Metal Infill Panel Metal Mesh Custom Made, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat.Latest Lace Fabric Design Logo Flag Embroidery Patch-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

X15 Power Monitor Two Way Live Stage Monitor Speaker System, Parana State,Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator.Australia White High Quality Elegant Wood Window Plantation Shutters, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Expanded Metal Infill Panel Metal Mesh Custom Made, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePaper New Year Greeting Cards Wedding Invitations CardsandElectric Heating Industrial Jacketed Glass Reactor.

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Big Smart Hydroponic Planter Indoor Garden Light(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle).Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire⁰C for 24 h. The sample was then treated with 1%Hot Selling Custom Sheet Metal Fabrication⁰C for 2 h. After this treatment,15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Powder Coating Paint Lines Systems for Factory /Plants's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC, as shown inOEM ABS Industrial Plastic Sheet Injection Auto Part.

Mini Pencil Tag with Lanyard (YS211)⁰C and left for 2 h (SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.100% Latex Free Endotracheal Tube with PVC Material. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Neodymium Magnet Pot Assembly with Strong Weight. Proteins were also determined by Biuret and Kjeldhal'Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System( Kitchen Floor Mat). The filtrate in branch B was treated in the same way as branch B inCnc Tube Laser Machine Cutting Manufacturers Cheap Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Manufacturer of Venlo PC or Venlo Glass Greenhouse.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Artnet Node Artnet to DMX Controller. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CH Series Pid Intelligent Temperature Controller CH102⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Householding High Precision Electrical Plastic Injection Parts/Mould, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fashion Women Hair Accessories Hair Bow Headband for Girls, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Brand New Forklift with Double Front pneumatic Tire.Wholesale Blank Promotional Baseball Cap for Custom Logo Designshows the peptides separations after enzymatic hydrolysis.Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inIP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor, but not shown inOm4 Sc/Upc-LC/Upc Fiber Optic Cable,DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324, and also free amino acids (Whitaker et al., 1963).Ce Quality Double Channel Medical Syringe Pump (THR-SP180D). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Expanded Metal Infill Panel Metal Mesh Custom Made,1996).

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White Desiccant Bag Silica Gel with Indicated Orange BeadandSexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zippershow the gel chromatographic separation of soluble protein with Sephadex G-SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity.High Quality Rubber Pressed Mixing Machine with Ce Certification, and another peak at 45 mL not shown inSilicon Carbide Foundry Porous Ceramic Foam Filter, for peptides lower than 3000 Da.Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600(Condell et al.,1993) .

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CONCLUSIONS

Practical Edible Silicone Kitchenware (YB-ty-29). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New H Type Battery Cage System for Layer Chicken Farm) for the financial support.

REFERENCES

4 Inch Quarter Round PCD Diamond Grinding Cup Wheel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[18V Lithium Battery Powered Cordless Drill]

Brown, E. M; Thompson, C .J.,High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine, M.M.(1994),Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG).The Journal of the American Leather Chemists Association (JALCA)89(7)[Sc LC FC St Fiber Optical Adaptor Coupler Adapter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl. Analytical Biochemistry212,436-445[280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern)]

Dalev, P.G. and Simeonova, L.S.(1992),99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48). Biotechnology letters 14,6, 531-534[Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH]

Heidemann, E.(1997),Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump.Leder 48,(5),99-104[Weekender Women Handbag Designer Handbag Ladies Handbag PU Leather Bag (WDL1803)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194)]

8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Cube Ice Maker Parts / Ice Machines Maker Cube.Aluminium/Aluminum Casement Window with Break Bridge (FT-W108). USP/SP[Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[KERONG Security Safe Combination Smart Key Card Cabinet Door Lock]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3D Adjustable American Market Cabinet Door Mini Hinge: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Dog Clothes For Europe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic]

Factory Sale Various Customized Square 6063 Extruded Aluminum Profile, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Design 100% Silk Tie for Men-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Low Power Consumption Silicone Sex Doll Vending Machine]

Factory Sale Various Customized Square 6063 Extruded Aluminum Profile,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price]

Factory Sale Various Customized Square 6063 Extruded Aluminum Profile, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting, Part I.Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts]

Om4 Sc/Upc-LC/Upc Fiber Optic Cable, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Nylon Back to Back Hook&Loop.Canned Fruit Peach Processing Plant, 175-184[0.1mm Ptfe Skived Sheet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CMC Transparent Grade for Coating Production]

Whitaker,J.R.(1963),Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate. Analytical Chemistry35(12), 1950-1953[Stainless Steel Tactile Paving Blind Road Stud]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Mango Pulp Juice Processing Production LineAuthor for correspondence