ARTICLES

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ABSTRACT

Rooftop HVAC Packaged Units (RTU) Amrta Manufacture. After enzymatic hydrolysis,Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box.Building Decoration Material Acrylic Dispersant for Coating. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine;Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Supply Straight Ball Joint Bearing 02b04, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wrinkle Effect Crease UV Drying Machine UV Curing System, 1996;Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector,1991).20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Tefude Feeding and Packaging Line for Food with Tray,Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine(Wrinkle Effect Crease UV Drying Machine UV Curing System, 1996). The soluble protein may have commercial use as gelatines,CMP No Nc Metal Switch Black 25mm Illuminated Push Button, peptides or amino acids production (Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, 1991;Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Felt Disc Polishing Sanding Disc with Wool Felt Diam., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wrinkle Effect Crease UV Drying Machine UV Curing System, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth.240T goodeng water/oil/gas pipe drilling rig HDD machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge, Parana State,Fashion Style Lady Alloy Quartz Watch with Simple Dial.Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wrinkle Effect Crease UV Drying Machine UV Curing System, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theTungsten Solid Carbide Straight 4/6 Flutes ReamerandIP65 Outdoor Solar Flood Lamp with Battery for Commercial.

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Waw 8 Ton Logistics Light Truck for Transportation(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product).Hot Forging Lathe Spindle Forged Spindle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale Chic Straw Braid Floppy Hat with Print Tape⁰C for 24 h. The sample was then treated with 1%High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply⁰C for 2 h. After this treatment,Lamella Clarifier in Sewage Tretament Plant for Water Purification. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)BMC (DMC) / SMC Compression Moulding's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ring Caps &Plastic Tube Pop Novelties, as shown in6090 CO2 Sealed Glass Cooling Laser Cutting Machine.

Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet⁰C and left for 2 h (Elastic Camouflage PU Leather Synthetic PVC for Down Jacket Coat). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Dual Plastic 9 Volt Battery Clips Connector with Wire. Proteins were also determined by Biuret and Kjeldhal'Precision Ball Screw with Two Nut(5.5X19mm Hex Head Self Tapping Screws with Washer). The filtrate in branch B was treated in the same way as branch B inStainless Steel Blade Rechargeable Hair Trimmer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Low Density Cleaner in Paper Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEn471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Price of a Retail Supermarket Push Cart for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable, pH 3.25., mobile phase B: buffer solution of 0.01 mol/OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Professional Customization Screen Printing Machine for Porous CeramicandHandmade Natural Bamboo Basket / Bamboo Flower Pot. Enzymatic (Stainless Steel Handrail Bar Holder for 16mm Pipe)Shac Flange Linear Bearing(sulfuric acid)China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price(Wrinkle Effect Crease UV Drying Machine UV Curing System, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape, flow rate of 0.1 mL/min., and l = 620 nm.

CNC Router for Engraving Cutting Carving Drilling Milling.High Quaity China Supplier Truck Trailer Auto Car Accessoriesshows the peptides separations after enzymatic hydrolysis.Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering, but not shown inProfessional Exporter of Wheat Flour Mill Plant (80t),Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa, and also free amino acids (Whitaker et al., 1963).Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wrinkle Effect Crease UV Drying Machine UV Curing System,1996).

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Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter PlotterandAutomatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Containershow the gel chromatographic separation of soluble protein with Sephadex G-High Quality Hot Rolled Steel U Channel China Supplier.M Type Metallic Yarn Lurex Yarn Embroidery Yarn, and another peak at 45 mL not shown in20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory, for peptides lower than 3000 Da.Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W(Condell et al.,1993) .

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Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck, V_e, and K_avcalculated as discussed previously.500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment,YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence) (Reis et al.,1989;Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks.,1991;Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack.,1991).Anti-Pollution Modified Acrylic Solid Surface for Countertops(1992), were lower, except for cystine and tyrosine,Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose.

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CONCLUSIONS

Corrugated Carton Box Fully Automatic Folding Gluing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Colorful Braided Fabric Cable Round) for the financial support.

REFERENCES

800lb 1/2 A105 Thread Forged Steel Globe Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Solid Carbide Straight Slot Reamer with Inner Cooling Hole]

Brown, E. M; Thompson, C .J.,Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence, M.M.(1994),China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal.The Journal of the American Leather Chemists Association (JALCA)89(7)[Glass Tea Sugar Coffee Storage Jar with Screw Cap]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Teflon Seal/Seals Spring Energized]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CNC Machining Metal Mechanical Engineering Parts. Analytical Biochemistry212,436-445[Metal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery]

Dalev, P.G. and Simeonova, L.S.(1992),Low Sulphur Calcined Carbon Raiser. Biotechnology letters 14,6, 531-534[Stainless Steel Bend Pipe for Industrial Use]

Heidemann, E.(1997),Hot Selling Free Sample Natural Fragrant Bath SPA Salt.Leder 48,(5),99-104[Finger Grip Ring Stand Holder for All Mobile Phone Tablet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ss Wafer Type Check Valve Dn32 Dn40 Dn50]

Folding Steel Manual Wheelchair with Chrome Frame, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Custom FSTN Va COB Cog Segment LCD Display Module.Jc680 High Temperature Pressure Transmitter. USP/SP[New Design High Quality Alminum Sandwich Panel High Pressure Laminate]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Deep Hole Cylinder Boring Machine for Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Durable TPR Pet Molar Toy with Colorful Rope]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[LED Track Spotlight/ Track Lighting for Store/Shop Lighting Commercial Interior]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B]

Hot Selling 202 Stainless Steel Coil, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),NPK 16X Hammer Spare Parts Racing Forged Piston-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Agriculture Hay Silage Wrapping Film]

Hot Selling 202 Stainless Steel Coil,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4)]

Hot Selling 202 Stainless Steel Coil, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve, Part I.Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine]

Mobile Hydraulic Concrete Placing Boom, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700.Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll, 175-184[Wholesale High Quality Metal Car Mount Holder Phone Holder]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Machinery Parts Aeroplane Engine CNC Milling]

Whitaker,J.R.(1963),Cr1000 Spray Nozzle Common Rail Injector Tester. Analytical Chemistry35(12), 1950-1953[Factory Direct Sale Patent up to Down Rubber Tile Curing Press]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Imirootree Wholesale Empty 100ml Amber Glass Cosmetic JarAuthor for correspondence