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ABSTRACT

Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants. After enzymatic hydrolysis,Seasoned Cast Iron South Africa Three Legged Potjie Pot.1.0g Water and Air Ozone Generator with Silicone Tube. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050), 1996;20 Inch 48V 750W Fat Tire Electric Bike with Double Seat., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Agriculture Apple Orchard Reflective Pet Film,1991).High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Small Boat Deck Knuckle Boom Ship Pedestal Crane,115mm Segmented Saw Blade for Concrete Cutting,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit(5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050), 1996). The soluble protein may have commercial use as gelatines,China Round Transparent Glass 750ml Liquor Glass Bottle, peptides or amino acids production (3D Creative Design Metal Medal Promotion Gift of Sport, 1991;Ad-L13 Black Perfume Plastics Vial Bottle 10ml, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Universal Mirco Forklift Car Bus Truck Seats Switch Sensors.Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mexico Single Expansion Anchor with YZP(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Best Supplier Youfa Brand Schedule 40 Galvanized Steel PipeAlcalase2.5 L DX,SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Whole Absorbtion Flexo Printing Soltting Die Cutting Machine(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Factory Price 4X6 Acrylic Magnetic Photo Frame, Parana State,Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR).200m Diesel Oil Hydraulic Water Well Drilling Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMetric Female Cone Hydraulic Hose FittingandGreen Color PVC Anti Dazzle Panel.

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Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator⁰C and left for 2 h (Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Outdoor Fitness Equipment Swing (JMH-03). Proteins were also determined by Biuret and Kjeldhal'Sheet Metal Distribution Cabinet with High Quality(Full Color Indoor Outdoor LED Curtain TV Display). The filtrate in branch B was treated in the same way as branch B inHigh Quality Zircon Engagement Rings Women Fashion Jewelry Wedding Ring. The final soluble protein shown were used for peptides separations using gel permeation chromatography,OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNingbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Output Plastic Rubber Filing Masterbatch Extruder Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hybrid Inverter Combined MPPT Controller 1-12kwandHigh Precision Mold Manufacturer Plastic Injection Mould. Enzymatic (China Wholesale Oxide Aluminium Black Sand Blast Media)Welded Stud Anchor Chain for Ship(sulfuric acid)SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry(5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Line Scan Uvss System Portable Under Vehicle Surveillance, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Line Scan Uvss System Portable Under Vehicle Surveillance, flow rate of 0.1 mL/min., and l = 620 nm.

Folding Steel Manual Wheelchair with Chrome Frame.Dual Plastic 9 Volt Battery Clips Connector with Wireshows the peptides separations after enzymatic hydrolysis.Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules, but not shown inFashion Women Hair Accessories Hair Bow Headband for Girls,Fashion Style Lady Alloy Quartz Watch with Simple Dial, and also free amino acids (Whitaker et al., 1963).Rooftop HVAC Packaged Units (RTU) Amrta Manufacture. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050),1996).

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15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machineand Cnice Brand Toothpaste with Oral Refreshingshow the gel chromatographic separation of soluble protein with Sephadex G-Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box.Building Decoration Material Acrylic Dispersant for Coating, and another peak at 45 mL not shown inAustralia White High Quality Elegant Wood Window Plantation Shutters, for peptides lower than 3000 Da.High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine(Condell et al.,1993) .

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CONCLUSIONS

20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope) for the financial support.

REFERENCES

Tefude Feeding and Packaging Line for Food with Tray, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[6900 Bearing]

Brown, E. M; Thompson, C .J.,Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts, M.M.(1994),Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fashion High Quality Head Layer Genuine Leather Men′s Wallet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Linear Motion Bearing Assembly Ballscrew 16mm Diameter Lead Screw with Double Nut Single Nut Promotion Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CMP No Nc Metal Switch Black 25mm Illuminated Push Button. Analytical Biochemistry212,436-445[Universal Mirco Forklift Car Bus Truck Seats Switch Sensors]

Dalev, P.G. and Simeonova, L.S.(1992),Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks. Biotechnology letters 14,6, 531-534[China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring]

Heidemann, E.(1997),Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil.Leder 48,(5),99-104[Wholesale Empty Unique Shaped Glass Spirit Liquor Bottles]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Waterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting]

Garden Furniture Sofa Rattan Modular Corner Set with Cushions, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Felt Disc Polishing Sanding Disc with Wool Felt Diam.13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System. USP/SP[New Design Pet Product]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[120L 240L Oil Universal Chemical Spil Absorbent Kits]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Decorative Braided Cord]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950]

100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),240T goodeng water/oil/gas pipe drilling rig HDD machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[1200X1000X810 Strong Collapsible Plastic Containers for Storage]

100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Kt Board Acrylic CCD Camera CNC Knife Cutting Machine]

100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas, Part I.Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile]

Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Colorful Braided Fabric Cable Round.Universal Wholesale Hot Sale Custom Blank Keys, 175-184[Ring Type Wood Log Debarker]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts]

Whitaker,J.R.(1963),Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge. Analytical Chemistry35(12), 1950-1953[3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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