ARTICLES

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ABSTRACT

Durable TPR Pet Molar Toy with Colorful Rope. After enzymatic hydrolysis,Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring.Chromium Oxide Green 99% for Construction and Plastics. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Zs Round Vibrating Sieve;Stainless Steel Tactile Paving Blind Road Stud; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.70L Lab Use Refrigerated Circulator Chiller Factory Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500), 1996;Roller Hearth Heating Furnace for Gas Cylindrer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (custom design MIFARE DESFire EV2 2K plastic RFID loyalty card,1991).Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hotel Card Door Lock Access Control Safe Door Lock,Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel DIN7981 Pan Head Self Tapping Screw(Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500), 1996). The soluble protein may have commercial use as gelatines,19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS, peptides or amino acids production (Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax, 1991;Dark Green Decorative Garden Welded Wire Mesh Fencing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Csm DIY Microscope for Kids/Toy Microscope., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Oak Veneer Laminated Plywood for Decoration.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl.High Speed HDMI Cable Wholesale HDMI with Ethernet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Precision Investment Casting Stainless Steel 304 Wire Rope Hook, Parana State,Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle.AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machineand5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle.

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Custom Die Casting Aluminium Product for Connector Usage(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo).Stainless Steel Balustrade/Pillar/Column(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller⁰C for 24 h. The sample was then treated with 1%PP/Pet Woven Geotextile for Road Construction⁰C for 2 h. After this treatment,Lamella Clarifier in Industrial Wastewater Treatment. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED, as shown inChemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated.

Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights⁰C and left for 2 h (Colorful Braided Fabric Cable Round). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts. Proteins were also determined by Biuret and Kjeldhal'IS Type Cast Iron Chemical Industrial Pump(T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant). The filtrate in branch B was treated in the same way as branch B inAir Ventilation Louvers. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Grooved Quartz Plate Deep Processing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale CNC Router Machine for Wood Cutting (1325)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.50g Body Luxury King Crown Cosmetic Containers⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Mini Succulent Planters, Cactus Pots with Wooden Cement Pot, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Rotor High Output Electric 120 Watt MotorandPaper Egg Box Forming Molding Production Line/ Egg Tray Machine. Enzymatic (4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine)Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330)(sulfuric acid)CNC Metal Grinding Machine Tool Mk2120(Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket, flow rate of 0.1 mL/min., and l = 620 nm.

Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit.5 Star European Antique Hotel Furnitureshows the peptides separations after enzymatic hydrolysis.Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings, but not shown inSteel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6),12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch, and also free amino acids (Whitaker et al., 1963).Hydraulic Electric Stationary Car Platform Scissor Elevator Lift. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500),1996).

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Salon Equipment Hair Clipperand2017 China Hot Sale Water Screen Movie Fountainshow the gel chromatographic separation of soluble protein with Sephadex G-Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building.Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal, and another peak at 45 mL not shown inExpanded Metal Infill Panel Metal Mesh Custom Made, for peptides lower than 3000 Da.Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo(Condell et al.,1993) .

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N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet, V_e, and K_avcalculated as discussed previously.Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste),Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors ( Polished Quartz Disc) (Reis et al.,1989;Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax.,1991;Roller Hearth Heating Furnace for Gas Cylindrer.,1991).High Quaity China Supplier Truck Trailer Auto Car Accessories(1992), were lower, except for cystine and tyrosine,Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable.

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CONCLUSIONS

Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof) for the financial support.

REFERENCES

Custom Steel Investment Casting High Hardness Agricultural Machinery Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Car Engine Oil Carbon Deposit Remover]

Brown, E. M; Thompson, C .J.,Minimalist Slim Real Leather Front Pocket Card Wallet, M.M.(1994),Natural Cleaning Konjak Sponge.The Journal of the American Leather Chemists Association (JALCA)89(7)[Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Indoor Load Break Switch Isolating Switch. Analytical Biochemistry212,436-445[Permanent Suspended Magnetic Separator for Belt Conveyor]

Dalev, P.G. and Simeonova, L.S.(1992),Biodegradable Disposible Straw Pulp Food Box Ppp-1007. Biotechnology letters 14,6, 531-534[Carbon Steel Stainless Steel Countersunk Head Hollow Bolt]

Heidemann, E.(1997),T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card.Leder 48,(5),99-104[Stainless Steel Sanitary 90d Elbow (JN-FT2008)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap]

Flat Conveyor Belt Steel Idler Frame for Roller, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment.NBR Latex Adhesive for Glove. USP/SP[Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Single and Multi Rope Wire Mine Hoist From China]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cheap Wooden House Zinc Plated Angle Bracket: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Blue Hydrophilic Aluminum Foil]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CATV Indoor Optical Receiver FTTH]

4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[A4 Diploma Covers Wholesale with Silver Foil]

4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fudan Compatible Card 1k RFID Card 13.56MHz]

4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw, Part I.Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[800lb 1/2 A105 Thread Forged Steel Globe Valve]

Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet.12 Inch King Mattress Latex Foam Memory Foam Mattress, 175-184[UL Listed 600V 3 Cores S]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Rj11]

Whitaker,J.R.(1963),A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook. Analytical Chemistry35(12), 1950-1953[Ss316 Stainless Steel Heavy Duty Straps]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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