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ABSTRACT

Waterproof PVDF Aluminum Curtain Wall Panel. After enzymatic hydrolysis,Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas.Round Modern Furniture Leisure Sofa for Promotion. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket;Sany Hg3333m Climbing Placing Boom; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Plastic Packaging Tray Vacuum Forming Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Permanent Suspended Magnetic Separator for Belt Conveyor, 1996;Double Sides Clear White Frosted Acrylic Sheet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery,1991).20W Solar Street Light with Lens Motion Sensor 2 Years Warranty. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC Machined Power Transmission Shaft Part,Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Good Price Channel Steel Used for Machinery(Permanent Suspended Magnetic Separator for Belt Conveyor, 1996). The soluble protein may have commercial use as gelatines,Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator, peptides or amino acids production (Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology, 1991;Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Direct Sale Patent up to Down Rubber Tile Curing Press., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Real Leather Wallet Mens Designer Leather Wallets. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Permanent Suspended Magnetic Separator for Belt Conveyor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338).Full Port Small Automatic Electronic Plumbing Ball Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers, Parana State,Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine.ASTM A240 304 316L No. 1 Stainless Steel Sheet, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Permanent Suspended Magnetic Separator for Belt Conveyor, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea TickandShenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen.

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12V 24V 220V Digital Temperature Controller(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Q-Sub Subwoofer Professional Passive Speaker Loud Speaker Woofer).Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%25kg Fertilizer Belt Packing Machine for Special Materials⁰C for 24 h. The sample was then treated with 1%Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses⁰C for 2 h. After this treatment,Rotor High Output Electric 120 Watt Motor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed, as shown inNeodymium Magnet Pot Assembly with Strong Weight.

Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine⁰C and left for 2 h (Clear PVC Roof Frame Tent in South Africa). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Rpl Straight Socket and Plugs Male Thread. Proteins were also determined by Biuret and Kjeldhal'46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall(China Soundproof Construction Foam PVC/WPC Board). The filtrate in branch B was treated in the same way as branch B inChina Aluminum Windows Cnc Double Head Cutting. The final soluble protein shown were used for peptides separations using gel permeation chromatography,OEM Metal Stamping Stainless Steel Parts with CNC Machining.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ss Wafer Type Check Valve Dn32 Dn40 Dn50. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/WPC Wood Plastic Board Equipment Extruder Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hot Sale CNC Router Machine for Wood Cutting (1325)andNeoprene Elbow Support. Enzymatic (Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules)CNC Produce Medical Machining Custom Metal Milling(sulfuric acid)Multi Sensor Nilitary Surveillance Thermal Imaging Security camera(Permanent Suspended Magnetic Separator for Belt Conveyor, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom Metal Stamping Welding Spare Parts for Farming, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom Metal Stamping Welding Spare Parts for Farming, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Hot Sale Dental Material Dental PMMA Block From China. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Marine Solas Adhesive Reflective Tape) for the financial support.

REFERENCES

Plastic Waste Pet Flakes Recycling Washing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[P8 Outdoor Video Ads LED Display Wall]

Brown, E. M; Thompson, C .J.,Deposited Stainless Steel Rivet Nut with Knurled, M.M.(1994),High Quality Stainless Steel Mechanical Filter Hosing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Colored Silicone NBR O-Rings Seals]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Electronic Foldable for Patient Treatment Ent Unit Chair]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Paper New Year Greeting Cards Wedding Invitations Cards. Analytical Biochemistry212,436-445[Stainless Steel Balustrade/Pillar/Column]

Dalev, P.G. and Simeonova, L.S.(1992),Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01). Biotechnology letters 14,6, 531-534[Custom Die Casting Aluminium Product for Connector Usage]

Heidemann, E.(1997),BLE5.0 Ttl to Bluetooth Module with at Command.Leder 48,(5),99-104[Wood Crushing Hammer Mill Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Foska Mini Size High Quality Heavy Duty Stapler]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fea040b New Arrival Airplane Seat Belt Extender: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Welding Stainless Steel Sheet Metal Welding Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fuel Pump Filter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Quick Connect Camlock and Grooved Couplings Quick Fitting Type D]

SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Square Shape Glass Candle Holder for One Piece Tealight Candle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Comfortable Men′s Winter Leather Snow Boots]

SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Laser ID Card Rigid PET Printing Sheet]

SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box, Part I.Medical Autoclavable Surgical Thoracoscopy Trocar. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Hamburgers]

DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Easy Foldable Kids Walking Chair Toys Baby Walker.Men V-Neck Longline T-Shirt with Color Block Ribbed Trims, 175-184[High Pressure Anti-Vibration Stainless Steel Flexible Corrugated Steam Metal Braided Hose]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated]

Whitaker,J.R.(1963),Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener. Analytical Chemistry35(12), 1950-1953[New Condition Hongyu Polystyrene Panel CNC Hot Wire Contour Foam Cutte]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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