ARTICLES

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ABSTRACT

Good Quality Hot Tube8 Japanese Stainless Steel Tube 666. After enzymatic hydrolysis,Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine.Round Plastic Hanging Basket (KD7014). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag;China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Design High Quality Alminum Sandwich Panel High Pressure Laminate, 1996;Steel Setractable Rail Protection Cover., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Natural Birch F&B Veneer Furniture Plywood,1991).Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Tire Repair Tool Kit, Tire Repair Strip,High Performance 915MHz PCB Antenna,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,GRP/FRP Profile Structures/Fiberglass Pultrusion Product(New Design High Quality Alminum Sandwich Panel High Pressure Laminate, 1996). The soluble protein may have commercial use as gelatines,Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel, peptides or amino acids production (High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering, 1991;Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customized Metal Material Products for Punching Stamping Part., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Design High Quality Alminum Sandwich Panel High Pressure Laminate, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L.Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Theme Park Inflatable Bouncer for Sale (BJ-B16)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

1 Ton Toyo Type Manual Chain Block, Parana State,600X600 mm Ugr 19 LED Panel Lights.Spark Resistant Construction Axial Fan with Fixed Pitch Blade, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (New Design High Quality Alminum Sandwich Panel High Pressure Laminate, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water FiltrationandOutdoor Multifunctional Solar Power Flashlight (SYSG-517).

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Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Small Coil Compression Spring in High Quality).3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25)⁰C for 24 h. The sample was then treated with 1%Precision CNC Milling DIN69871. Mas403 for CNC Machinist⁰C for 2 h. After this treatment,Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Building Material of Granite Ceramic Wall Tile for Wall Decoration's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine, as shown inIndoor LED Track Light (AP-TB30/AP-TB20/AP-TB10).

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Melee Custom Plastic Mould New Design. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/White Desiccant Bag Silica Gel with Indicated Orange Bead, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Iron Wire Mesh Chain Link Mesh Security Fence, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera.Poultry Farming Equipment Broiler Chicken Ground Floor Rearing Systemshows the peptides separations after enzymatic hydrolysis.Wholesale Flower Embroidery Applique Patches(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFull Automatic Paper Lunch Food Box Carton Forming Machine, but not shown inSt Fiber Optic Adaptor,Big Bussiness Project Commerical Chicken Poultry Farm Buildings, and also free amino acids (Whitaker et al., 1963).400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (New Design High Quality Alminum Sandwich Panel High Pressure Laminate,1996).

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CONCLUSIONS

Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court) for the financial support.

REFERENCES

High Quality Mini LED Flood Light SMD Flood Light 50W, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Lumber Grinder]

Brown, E. M; Thompson, C .J.,Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead, M.M.(1994),Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sell Data USB Connector Type C 12 Pin Terminal Block]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[50g Body Luxury King Crown Cosmetic Containers]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Professional Manufacture Metal Medal Promotional Gift. Analytical Biochemistry212,436-445[2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards]

Dalev, P.G. and Simeonova, L.S.(1992),Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving. Biotechnology letters 14,6, 531-534[20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21]

Heidemann, E.(1997),Singlemode Su Upc Fiber Optic Fast Connector.Leder 48,(5),99-104[Shopping Box]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Precision Mold Manufacturer Plastic Injection Mould]

100 Liter Cooking Pots for Jam, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Roller Skating Sneakers Shoes for Children (8083).Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder. USP/SP[WPC Wood Plastic Board Equipment Extruder Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[40X-1600X Biological Microscope Xsz-107V LED Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Electric Heating Industrial Jacketed Glass Reactor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pvc Motor Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Best Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for Smelting]

40X-1600X Biological Microscope Xsz-107V LED Light, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),6063 Aluminum Coil for Commercial Vehicle Floor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Tire Repair Strip]

40X-1600X Biological Microscope Xsz-107V LED Light,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making]

40X-1600X Biological Microscope Xsz-107V LED Light, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Silicon Carbide Foundry Porous Ceramic Foam Filter, Part I.1200X1000X810 Strong Collapsible Plastic Containers for Storage. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Acid Water Pulse Output Liquid Orifice Type Flow Meter]

Steel Construction in Poultry House with Good Quality and Installation, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White.10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle, 175-184[m.zhiyounewmaterial.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane]

Whitaker,J.R.(1963),Stone Cracking Rock Cutting Cement. Analytical Chemistry35(12), 1950-1953[Meat Machinery/Chicken Breast and Fish Slicing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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