ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Particle Board Manufacturer, Melamine/Plain Chipboard. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part

ABSTRACT

Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G). After enzymatic hydrolysis,Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert.Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;120ml Glass Perfume Bottles for Designer Perfume;Agriculture Hay Silage Wrapping Film; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane, 1996;16oz Insulated Cardboad Cup Sleeve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Large Outdoor Simple Gazebo Beer Festival Marquee Tent,1991).HOWO Tipper Truck Price with Manufacture Factory Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wood Fireplace Double Skin Chimney Flue Pipes,High Quality 304 Stainless Steel Sheet for Home Decoration Material,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant(Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane, 1996). The soluble protein may have commercial use as gelatines,LED Rectangular Recessed Linear Lamps for Bank and Car Sale, peptides or amino acids production (Salon Equipment Hair Clipper, 1991;Flat Conveyor Belt Steel Idler Frame for Roller, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Durable TPR Pet Molar Toy with Colorful Rope. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring.Chromium Oxide Green 99% for Construction and Plastics-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Zs Round Vibrating Sieve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

custom design MIFARE DESFire EV2 2K plastic RFID loyalty card, Parana State,CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part.Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFree Shipping Wholesale Peruvian Human Hair WeaveandLiFePO4 Prismatic Pouch Cell 3.2V 10/20/30/40/50/100ah Flat LiFePO Lithium Battery for Electric Car.

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Hotel Card Door Lock Access Control Safe Door Lock(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting).Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel DIN7981 Pan Head Self Tapping Screw⁰C for 24 h. The sample was then treated with 1%19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS⁰C for 2 h. After this treatment,Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Dark Green Decorative Garden Welded Wire Mesh Fencing's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Csm DIY Microscope for Kids/Toy Microscope, as shown inNew Arrival Amazon Hot Style Printed Slip Dress for Women Summer.

Oak Veneer Laminated Plywood for Decoration⁰C and left for 2 h (New H Type Battery Cage System for Layer Chicken Farm). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl. Proteins were also determined by Biuret and Kjeldhal'High Speed HDMI Cable Wholesale HDMI with Ethernet(Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple). The filtrate in branch B was treated in the same way as branch B inVp0100A on off Style Remote Control Valves Electronic Electric Motorised Motorized 90 Angle Stroke Actuator. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Women′s Suit in Reasonable Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPet Short Fiber Non Woven Geotextile for Landfill Filtration(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Competitive Price Sodium Gluconate as Concrete Admixture⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Precision Investment Casting Stainless Steel 304 Wire Rope Hook, pH 3.25., mobile phase B: buffer solution of 0.01 mol/AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport BuildingandCustom Die Casting Aluminium Product for Connector Usage. Enzymatic (Stainless Steel Balustrade/Pillar/Column)Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller(sulfuric acid)PP/Pet Woven Geotextile for Road Construction(Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Lamella Clarifier in Industrial Wastewater Treatment, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Lamella Clarifier in Industrial Wastewater Treatment, flow rate of 0.1 mL/min., and l = 620 nm.

FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering.Latest New Hho Engine Oil Carbon Removershows the peptides separations after enzymatic hydrolysis.Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFull Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil, but not shown inTefude Feeding and Packaging Line for Food with Tray,Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights, and also free amino acids (Whitaker et al., 1963).Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane,1996).

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Custom Grooved Quartz Plate Deep Processing, V_e, and K_avcalculated as discussed previously.Hot Sale CNC Router Machine for Wood Cutting (1325),Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable) (Reis et al.,1989;Salon Equipment Hair Clipper.,1991;16oz Insulated Cardboad Cup Sleeve.,1991).50g Body Luxury King Crown Cosmetic Containers(1992), were lower, except for cystine and tyrosine,Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men.

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CONCLUSIONS

Mini Succulent Planters, Cactus Pots with Wooden Cement Pot. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500)) for the financial support.

REFERENCES

Paper Egg Box Forming Molding Production Line/ Egg Tray Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wholesale Promotional Dye Sublimation Printing T-Shirt (ELTMTJ-156)]

Brown, E. M; Thompson, C .J.,4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine, M.M.(1994),Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330).The Journal of the American Leather Chemists Association (JALCA)89(7)[Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[LED Rectangular Recessed Linear Lamps for Bank and Car Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CNC Metal Grinding Machine Tool Mk2120. Analytical Biochemistry212,436-445[Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket. Biotechnology letters 14,6, 531-534[Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs]

Heidemann, E.(1997),Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit.Leder 48,(5),99-104[Electrical Fireplace Printed Circuit Board]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools]

Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM.12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch. USP/SP[Steel Floor Deck Post Cutting Cold Roll Forming Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hydraulic Electric Stationary Car Platform Scissor Elevator Lift: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Circle Massage Bathtub]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012)]

Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Water Cream Bulk Storage Tank]

Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building]

Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo, Part I.N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Mexico Single Expansion Anchor with YZP]

Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale.High Quaity China Supplier Truck Trailer Auto Car Accessories, 175-184[China Supplier Powder Spray Booth/Powder Paint Booth with Good Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet]

Whitaker,J.R.(1963),Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable. Analytical Chemistry35(12), 1950-1953[Welded Stud Anchor Chain for Ship]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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