ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Stainless Steel Balustrade/Pillar/Column. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller

ABSTRACT

PP/Pet Woven Geotextile for Road Construction. After enzymatic hydrolysis,Lamella Clarifier in Industrial Wastewater Treatment.FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts, 1996;IS Type Cast Iron Chemical Industrial Pump., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine,1991).Custom Grooved Quartz Plate Deep Processing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sale CNC Router Machine for Wood Cutting (1325),Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,50g Body Luxury King Crown Cosmetic Containers(Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts, 1996). The soluble protein may have commercial use as gelatines,Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men, peptides or amino acids production (Mini Succulent Planters, Cactus Pots with Wooden Cement Pot, 1991;Paper Egg Box Forming Molding Production Line/ Egg Tray Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CNC Metal Grinding Machine Tool Mk2120. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket.Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo, Parana State,Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller.N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the Hose Fittingsand Printed Socks.

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Natural Cleaning Konjak Sponge⁰C and left for 2 h (Printing Pure Color Women Fitness Wear). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Indoor Load Break Switch Isolating Switch. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Biodegradable Disposible Straw Pulp Food Box Ppp-1007. Proteins were also determined by Biuret and Kjeldhal'T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card( Diatomite Earth Beer Filter). The filtrate in branch B was treated in the same way as branch B inm.solwaysolarlight.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%NBR Latex Adhesive for Glove. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCheap Wooden House Zinc Plated Angle Bracket(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding MachineandLift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer. Enzymatic (500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet)12 Inch King Mattress Latex Foam Memory Foam Mattress(sulfuric acid)A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook(Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance, flow rate of 0.1 mL/min., and l = 620 nm.

Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt.New Scba Breathing Apparatus Equipment with Ce Certificationshows the peptides separations after enzymatic hydrolysis.Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEmpty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer, but not shown inWholesales Stackable Coffee Iron Chair,Longyi Crimping Types Cable Terminal Lugs, and also free amino acids (Whitaker et al., 1963).Rigid PVC Foam Board for FRP Marine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts,1996).

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CONCLUSIONS

Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building) for the financial support.

REFERENCES

China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Expansive Mortar Hight Range Soundless Cracking Agent]

Brown, E. M; Thompson, C .J.,300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve, M.M.(1994),Plastic Injection Molding Companies China for Tablet PC Case.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mini Succulent Planters]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools. Analytical Biochemistry212,436-445[Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Solid Tungsten Carbide Rods. Biotechnology letters 14,6, 531-534[Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump]

Heidemann, E.(1997),Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover.Leder 48,(5),99-104[impact crusher parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PP Coated Pipe Machine Extruder Machine]

Custom Die Casting Aluminium Product for Connector Usage, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved.High Tensisle Strength Tissue Paper. USP/SP[100 Liter Cooking Pots for Jam]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Medical Autoclavable Surgical Thoracoscopy Trocar]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Tractor Mounted Beach Cleaning Equipment Large Size: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Temperature Side Opening AAC Autoclave]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Large Size Swimsuit]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber]

100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Waterjet 87K Intensifier Part Check Valve Body 020077-1]

100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine]

100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PP Coated Pipe Machine Extruder Machine, Part I.China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Small Table CNC Marble Engraver for Stone]

Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mining Machinery Parts Diamond Saw Blade Segments for Marble.Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface, 175-184[Laser Cutting Machning]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof]

Whitaker,J.R.(1963),Multi Column Load Cell 100kg, 20t. Analytical Chemistry35(12), 1950-1953[Ad-L13 Black Perfume Plastics Vial Bottle 10ml]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hot Sale High-Order Elasticty Girls Sports Wear Yaga SuitAuthor for correspondence