ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Welded Stud Anchor Chain for Ship. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry

ABSTRACT

Line Scan Uvss System Portable Under Vehicle Surveillance. After enzymatic hydrolysis,Folding Steel Manual Wheelchair with Chrome Frame.Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Building Decoration Material Acrylic Dispersant for Coating, 1996;High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades,1991).Factory Supply Straight Ball Joint Bearing 02b04. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wrinkle Effect Crease UV Drying Machine UV Curing System,Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector(Building Decoration Material Acrylic Dispersant for Coating, 1996). The soluble protein may have commercial use as gelatines,20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory, peptides or amino acids production (Tefude Feeding and Packaging Line for Food with Tray, 1991;Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CMP No Nc Metal Switch Black 25mm Illuminated Push Button.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Building Decoration Material Acrylic Dispersant for Coating, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil.Felt Disc Polishing Sanding Disc with Wool Felt Diam-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black CableAlcalase2.5 L DX,100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-240T goodeng water/oil/gas pipe drilling rig HDD machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength, Parana State,SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry.Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Building Decoration Material Acrylic Dispersant for Coating, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theNew CCTV 4.0MP H. 265+ IR Bullet Waterproof IP CameraandVertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box.

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Colorful Braided Fabric Cable Round(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s).Universal Wholesale Hot Sale Custom Blank Keys(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge⁰C for 24 h. The sample was then treated with 1%Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30⁰C for 2 h. After this treatment,Waw 8 Ton Logistics Light Truck for Transportation. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Forging Lathe Spindle Forged Spindle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sale Chic Straw Braid Floppy Hat with Print Tape, as shown inMining Machinery Stone Crusher Bowl Liner Cone Crusher Parts.

High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply⁰C and left for 2 h (Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Lamella Clarifier in Sewage Tretament Plant for Water Purification. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,BMC (DMC) / SMC Compression Moulding. Proteins were also determined by Biuret and Kjeldhal'Ring Caps &Plastic Tube Pop Novelties(Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool). The filtrate in branch B was treated in the same way as branch B inPet Foam Bottle with Pump for Facial Cleanser 200ml. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDual Plastic 9 Volt Battery Clips Connector with Wire(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Low Density Cleaner in Paper Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

8.0 Inch Binocular Living Body Detection Face Recognition Access Control CameraandCustomized Stainless Steel Laser Cutting. Enzymatic (Price of a Retail Supermarket Push Cart for Sale)Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable(sulfuric acid)OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate(Building Decoration Material Acrylic Dispersant for Coating, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Handmade Natural Bamboo Basket / Bamboo Flower Pot, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Handmade Natural Bamboo Basket / Bamboo Flower Pot, flow rate of 0.1 mL/min., and l = 620 nm.

Stainless Steel Handrail Bar Holder for 16mm Pipe.Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitationshows the peptides separations after enzymatic hydrolysis.Shac Flange Linear Bearing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover, but not shown inRound Modern Furniture Leisure Sofa for Promotion,China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price, and also free amino acids (Whitaker et al., 1963).Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Building Decoration Material Acrylic Dispersant for Coating,1996).

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Good Price Thermoplastic Hot Melt Road Marking Paint Line EquipmentandLamella Clarifier in Industrial Wastewater Treatmentshow the gel chromatographic separation of soluble protein with Sephadex G-CNC Router for Engraving Cutting Carving Drilling Milling.Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine, and another peak at 45 mL not shown in Powder, for peptides lower than 3000 Da.Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa(Condell et al.,1993) .

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CONCLUSIONS

500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (240T goodeng water/oil/gas pipe drilling rig HDD machine) for the financial support.

REFERENCES

YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[1L Super Critical CO2 Oil Extraction Machine for Cbd Oil]

Brown, E. M; Thompson, C .J.,Anti-Pollution Modified Acrylic Solid Surface for Countertops, M.M.(1994),Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose.The Journal of the American Leather Chemists Association (JALCA)89(7)[2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Zs Round Vibrating Sieve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Corrugated Carton Box Fully Automatic Folding Gluing Machine. Analytical Biochemistry212,436-445[Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby]

Dalev, P.G. and Simeonova, L.S.(1992),800lb 1/2 A105 Thread Forged Steel Globe Valve. Biotechnology letters 14,6, 531-534[ 20t]

Heidemann, E.(1997),Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence.Leder 48,(5),99-104[Hardware]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Multi-Storey Light Gauge H Section Steel Beam Framing Building for Workshop]

China Wholesale Oxide Aluminium Black Sand Blast Media, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal.CNC Machining Metal Mechanical Engineering Parts. USP/SP[360 Degree Maverick Angler Fishing Kayak with Rotating Seat]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Low Sulphur Calcined Carbon Raiser: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Outdoor Playground Plastic Slide with Swing and Basketry]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[600ml Collapsible Folding Silicone Sports Water Bottle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Big Dimension UHP Graphite Electrodes for Steel Making]

Hot Selling Free Sample Natural Fragrant Bath SPA Salt, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom FSTN Va COB Cog Segment LCD Display Module-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Promotion Snack Children School Lunch Bag Cooler Bag]

Hot Selling Free Sample Natural Fragrant Bath SPA Salt,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Neodymium Magnet with RoHS ISO 16499 Approved]

Hot Selling Free Sample Natural Fragrant Bath SPA Salt, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Jc680 High Temperature Pressure Transmitter, Part I.Deep Hole Cylinder Boring Machine for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Corrugated Carton Box Fully Automatic Folding Gluing Machine]

Hot Selling 202 Stainless Steel Coil, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),NPK 16X Hammer Spare Parts Racing Forged Piston.2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve, 175-184[.075kn vibrating motor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Timber Pressure Vessel for Wood Processing]

Whitaker,J.R.(1963),Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector. Analytical Chemistry35(12), 1950-1953[LED Outdoor Solar Lawn Garden Lighting for Wall]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on SaleAuthor for correspondence