ARTICLES

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ABSTRACT

High Pulling Force Rotatable Rare Earth Crane Magnet Hook. After enzymatic hydrolysis,1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products.Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Standard Industrial Double Nut Ball Screw Dfu Series, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter, 1996;Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customized Logo Outdoor Hanging Swinging Camping Hammock,1991).EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning,Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Metal Detector for Gold, Silver, etc Underground Metel Detector(Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter, 1996). The soluble protein may have commercial use as gelatines,Hing Quality Rubber O Ring in Motor High Pressure, peptides or amino acids production (Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe, 1991;Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom.Free Shipping Wholesale Peruvian Human Hair Weave-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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NSK

500ml Blue Glass Beer Bottle with Crown Cap(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Nbm High Quality Auto Part Factory Price 6pk2310).White Iron Wear Crusher Blades 63HRC(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System⁰C for 24 h. The sample was then treated with 1%Stone Carving Flower Pot for Garden Decoration⁰C for 2 h. After this treatment,2018 New Fashion Men Canvas Messenger Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Purity Sodium Bicarbonate with White Powder Type's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304, as shown inSwf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formations.

IP65 Outdoor Solar Flood Lamp with Battery for Commercial⁰C and left for 2 h (Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Carbide Rod H6 Tc Rods. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Mens Slim Wallet Custom Design Leather Wallet Men. Proteins were also determined by Biuret and Kjeldhal'St Fiber Optic Adaptor(LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6). The filtrate in branch B was treated in the same way as branch B inHospital Oxygen Producing Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Customized Cute Animal Dinosaur Kids Baby Baseball Cap.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Latest Fashion Best-Selling Marble Slab Cutting Saw Blades. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBlack Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Motorcycle Tube Making Machine/Inner Tube Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Paper Automaitc Carton Box Cardboard Strapping Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Motor Company Produce BLDC Fan Motor for Barn Vent Fan, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment.Top Furniture Hotel Dining Furniture Metal Hotel Table Chairshows the peptides separations after enzymatic hydrolysis.Rht-060ws Soda Process Water Cooled Screw Type Water Chiller(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPower Plant Seawater Level Measurement Integrated Pressure Transducer, but not shown inGood Price High Torque 12mm Low Speed Small Geared Motor,Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp, and also free amino acids (Whitaker et al., 1963).Boat Aluminum Pipe 5083 Marine Used Aluminum Tube. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter,1996).

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Flanged Braided Metal Hose Connector, V_e, and K_avcalculated as discussed previously.Timber Pressure Vessel for Wood Processing,1200mm Recessed Mounted LED Linear Light. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (FC Bare Fiber Optic Adapter for Network) (Reis et al.,1989;Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe.,1991;Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card.,1991).The Big Diameter Carbide Tipped Hole Saw(1992), were lower, except for cystine and tyrosine,Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo.

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CONCLUSIONS

Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve) for the financial support.

REFERENCES

Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom French Liebherr Large Counterweight Iron]

Brown, E. M; Thompson, C .J.,Fashion Men′s Large Dial Casual Quartz Watch, M.M.(1994),PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Handrail Bar Holder for 16mm Pipe]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Outdoor Garden Furniture Baby Plastic Swing for Sale. Analytical Biochemistry212,436-445[PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence]

Dalev, P.G. and Simeonova, L.S.(1992),Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone. Biotechnology letters 14,6, 531-534[Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence]

Heidemann, E.(1997),2D LED Rope Lighting Motif Lights for Christmas Decoration.Leder 48,(5),99-104[High Quality Steel Grating Cover]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration]

Neodymium Magnet with RoHS ISO 16499 Approved, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Modern Compact Laminate Key Lock Changing Room Locker.Outdoor Full Color LED Video Wall. USP/SP[Outdoor Multifunctional Solar Power Flashlight (SYSG-517)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cross Hex Flange Screw]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Small Coil Compression Spring in High Quality]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fullx connector Outdoor Cable Assembly Matching Ericsson Rru Equipment Fullaxs]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10)]

Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Stainless Steel Mechanical Filter Hosing]

Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel]

Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Quick Connect Camlock and Grooved Couplings Quick Fitting Type D, Part I.High Quality White Calcined Alumina Powder for Ceramic Industry. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Professional Various Standard Hot Sale Rotary Lab Single Round Punch]

500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),100kw Cummins Diesel Generator Set.Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle, 175-184[Aluminum Die Cast Junction Box]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System]

Whitaker,J.R.(1963),Luxury Hotel Furniture Antique Design Marble Side Table. Analytical Chemistry35(12), 1950-1953[Full Automatic Paper Lunch Food Box Carton Forming Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

St Fiber Optic AdaptorAuthor for correspondence