ARTICLES

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ABSTRACT

Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment. After enzymatic hydrolysis,Steel Pipe Rust Removal Shot Blasting Cleaning Machine.Hydraulic Press Corrugated Cold Roll Automatically Forming Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring, 1996;300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic Injection Molding Companies China for Tablet PC Case,1991).27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Solid Tungsten Carbide Rods,Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved(China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring, 1996). The soluble protein may have commercial use as gelatines,High Tensisle Strength Tissue Paper, peptides or amino acids production (Tractor Mounted Beach Cleaning Equipment Large Size, 1991;100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PP Coated Pipe Machine Extruder Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances.Mining Machinery Parts Diamond Saw Blade Segments for Marble-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Multi Column Load Cell 100kg, 20tAlcalase2.5 L DX,LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Foldable PP Plastic Storage Logistics Turnover Box(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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High Quality Flange Dismantling Joint(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes).Duplex Stainless Steel Floating Ball Valve(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card⁰C for 24 h. The sample was then treated with 1%Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine⁰C for 2 h. After this treatment,Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC Machine Aluminum Telescopic Covers Flat Bellows Cover, as shown inPlastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder.

7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input⁰C and left for 2 h (CREE Chip 24W 28W 30W LED Downlight). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Kubota Combine Harvester Spare Parts 5h484-2392 Nut. Proteins were also determined by Biuret and Kjeldhal'Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable(FC Bare Fiber Optic Adapter for Network). The filtrate in branch B was treated in the same way as branch B ina-topmachine.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Steel Floor Deck Post Cutting Cold Roll Forming Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSimilar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern), pH 3.25., mobile phase B: buffer solution of 0.01 mol/PS Foam Disposable Fast Food Box Making Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

304L Stainless Steel U Channel Good Performance. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck) for the financial support.

REFERENCES

Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[12 Oz Sweet Color New Bone China Ceramic Coffee Mug]

Brown, E. M; Thompson, C .J.,Junior Backyard Training Practise Soccer Goal Net, M.M.(1994),350mm Diamond Blade for Granite.The Journal of the American Leather Chemists Association (JALCA)89(7)[Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Professional Various Standard Hot Sale Rotary Lab Single Round Punch]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Welding Stainless Steel Sheet Metal Welding Parts. Analytical Biochemistry212,436-445[Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System]

Dalev, P.G. and Simeonova, L.S.(1992),Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp. Biotechnology letters 14,6, 531-534[Full Automatic Paper Lunch Food Box Carton Forming Machine]

Heidemann, E.(1997),Laser ID Card Rigid PET Printing Sheet.Leder 48,(5),99-104[180W 130lm/W Die-Casting Aluminium Housing LED Street Light]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[St Fiber Optic Adaptor]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Melamine Paper Manufacturer for Decoration]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Types Of Galvanized Iron Sheet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light]

Ss316 Stainless Steel Heavy Duty Straps, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Digger Spring for Excavator Machinery]

Ss316 Stainless Steel Heavy Duty Straps,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180)]

Ss316 Stainless Steel Heavy Duty Straps, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Temperature Side Opening AAC Autoclave, AAC Block Making Machine, Part I.PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CNC Machining Metal Mechanical Engineering Parts]

Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal.1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine, 175-184[Outdoor Playground Amusement Equipment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable]

Whitaker,J.R.(1963),New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar. Analytical Chemistry35(12), 1950-1953[Hot Sell Data USB Connector Type C 12 Pin Terminal Block]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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