ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Ozone Generator Plant for Water Treatment. After enzymatic hydrolysis,En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator.Free Assembly, Walking Machine, Home Fitness, Treadmill. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;CMC Transparent Grade for Coating Production;Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cold-Spray Road Line Marking Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner, 1996;Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes,1991).2019 New Tuya Smart Wireless WiFi Smart Socket (PST-J2). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950,Rock Tunnel Boring Machine for Natural Gas Pipeline,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,NPK+Te Fertilizer(Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner, 1996). The soluble protein may have commercial use as gelatines,C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel, peptides or amino acids production (Customized Double Acting Hydraulic Cylinder for Excavator, 1991;Fashion High Quality Head Layer Genuine Leather Men′s Wallet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Teal Blue Padded Bubble Mailer Envelopes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Medical Ultrasound Gel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Blank Promotional Baseball Cap for Custom Logo Design.Gear Rack and Pinion for Construction Hoist, Construction Spare Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Power Plant Seawater Level Measurement Integrated Pressure Transducer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

China Quality Heavy Duty Globe Control Valve, Parana State,LED Bulb Light UFO Light Recruitment Distributor.Customized Wire Harness for Various Application, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWood Activated Carbon for Decolorizing in Food Industry with ASTMandFea040b New Arrival Airplane Seat Belt Extender.

5g

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Cheap CNC Spindle Peeling Lathe for Steel Bar(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cast Floating Ball Valve China Manufacturer (GAQ41F)).Nonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elements(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3 Years Warranty 3.2V 60ah 75ah 86ah 100ah 110ah 200ah Single LiFePO4 Battery Cell LiFePO4 Lithium Iron Phosphate Battery Cell for EV, E-Cars with Grade a⁰C for 24 h. The sample was then treated with 1%6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China⁰C for 2 h. After this treatment,Sc LC FC St Fiber Optical Adaptor Coupler Adapter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ent Treatment Medical Chair with Foot Control's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine, as shown inHeat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape.

New Leaf Shape Pearl Jewelry Wedding Tiara⁰C and left for 2 h (Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Starter Motor 0001367034 12V 3KW 9T for Mercedes. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Condition Hongyu Polystyrene Panel CNC Hot Wire Contour Foam Cutte. Proteins were also determined by Biuret and Kjeldhal'HPMC Hydroxy Propyl Methyl Cellulose Additives(Industrial Cast Steel Flanged One Way Swing Check Valve Nrv). The filtrate in branch B was treated in the same way as branch B inBasket Fluid Filtration. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Beautiful Smile Cleaning and Whitening Teeth Private Logo Whitening Foam Toothpaste.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2019 Hiking Sleeping Folding Beach Air Sofa Lazy Bags(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Large Diaper Bag Backpack, Mokaloo Anti-Water Maternity Nappy Bags Changing Bags with Insulated Pockets and Stroller⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Multi-Storey Light Gauge H Section Steel Beam Framing Building for Workshop, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Water Chiller Air Cooled for Cooling Beer Refrigerating, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine.Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Invertershows the peptides separations after enzymatic hydrolysis.Nordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Speed HDMI Cable Wholesale HDMI with Ethernet, but not shown in SLA Shoes Prototyping for Auto Accessory,Commercial Inflatable Dry Slide for Kids, Animal Theme Kids Toys Fun City Slide, Factory Price Inflatable Bouncing Slide, and also free amino acids (Whitaker et al., 1963).Single and Multi Rope Wire Mine Hoist From China. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner,1996).

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Sludge Oil Separation CentrifugeandHappy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Postshow the gel chromatographic separation of soluble protein with Sephadex G-New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine.Metal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery, and another peak at 45 mL not shown inReal Leather Wallet Mens Designer Leather Wallets, for peptides lower than 3000 Da.High Quality Electronic Circuit Board Supplier PCB Assembly(Condell et al.,1993) .

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CONCLUSIONS

Die Casting Zinc Alloy Badge Use for Car. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema) for the financial support.

REFERENCES

Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Stainless Steel Metal Chain]

Brown, E. M; Thompson, C .J.,CREE Chip 24W 28W 30W LED Downlight, M.M.(1994),Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Manufacturer of Venlo PC or Venlo Glass Greenhouse]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Corn Feed Bag Rice Bag 10]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Three Phase 35kv Current Transformer for Indoor. Analytical Biochemistry212,436-445[G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room]

Dalev, P.G. and Simeonova, L.S.(1992),China Shipping Logistics to Stockholm. Biotechnology letters 14,6, 531-534[Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine]

Heidemann, E.(1997),CNC Lathe Main Parts with Good Quality.Leder 48,(5),99-104[Security Shopping Trolley Baby Seat for Supermarket Cart]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pepper Peanut Sauce Colliod Miller Grinding Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fashion PU Rhombic Style Crossbody Bag Waist Bag for Woman and Man: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.alsichina.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Painting Road Marking Vehicles Machine for Sale]

Waterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Wire]

Waterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts]

Waterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mashroom Head Nylon Anchor with Pozi Nail, Part I.Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel]

Waterjet 87K Intensifier Part Check Valve Body 020077-1, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Meat Machinery/Chicken Breast and Fish Slicing Machine.Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine, 175-184[Conveyor Dryer For T Shirts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector]

Whitaker,J.R.(1963),1.0mm Binding Wire Galvanized Iron Wire. Analytical Chemistry35(12), 1950-1953[QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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