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ABSTRACT

Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz. After enzymatic hydrolysis,Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator.Carbon Steel Stainless Steel Countersunk Head Hollow Bolt. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle, 1996;Clear PVC Roof Frame Tent in South Africa., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511),1991).Stainless Steel Bend Pipe for Industrial Use. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ybz-E16f20 Vane Steering Hydraulic Pumps,Good Quality Snow White Pumpkin Seeds for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors(5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle, 1996). The soluble protein may have commercial use as gelatines,Automotive Acrylic Wholesale Masking Tape Factory Price, peptides or amino acids production (Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price, 1991;Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Watermrk Drop in Bathroom Basin Sink., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.OEM ABS Industrial Plastic Sheet Injection Auto Part.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-15L Big Cooling Cylinder High Production Hard Ice Cream Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Flange Type Ball Float Water Tank Control Valve.925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould, Parana State,Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box.High Pressure Braided Rubber Oil Hose, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSteel Pipe Rust Removal Shot Blasting Cleaning MachineandMining Machinery Parts Diamond Saw Blade Segments for Marble.

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High Quality Beech Wood Chiavari Chair at a Competitive(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Outdoor Garden Furniture Baby Plastic Swing for Sale).Gradient Print Overlay Push Button Membrane Keypad Switch(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X⁰C for 24 h. The sample was then treated with 1%Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004)⁰C for 2 h. After this treatment,Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle, as shown inElectric Manual Ice Crusher Ice Shaver Industrial Ice Machine.

Low Power Consumption Silicone Sex Doll Vending Machine⁰C and left for 2 h (a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Toughened Glass Disc Suspension Insulator. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller. Proteins were also determined by Biuret and Kjeldhal'63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter(Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml). The filtrate in branch B was treated in the same way as branch B inWaterproof ABS Uid Programmable Rewritable Em4305 125 kHz Proximity RFID Keyfob / Key Fob Tag. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Customized Round Airless Lotion Pump Bottles for Skin Care Products.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Round Shape Dinner Football Gift Bowl with Cover Plate. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avProfessional Exporter of Wheat Flour Mill Plant (80t)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aluminum Alloy Rack for Conductor, Wire, Cable, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outdoor Accessories Fishing Hunting Masks, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Baby Clothes Wear Fleece Baby Bunting Onesie Jacket, V_e, and K_avcalculated as discussed previously.Life Size 170cm Silicone Sex Dolls Real Love Doll for Male,Reflective Sheeting Material for Licence Plate. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Easy Foldable Kids Walking Chair Toys Baby Walker) (Reis et al.,1989;Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price.,1991;Clear PVC Roof Frame Tent in South Africa.,1991).Industrial Grade Nitrous Oxide for Hot Sales(1992), were lower, except for cystine and tyrosine,Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove.

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CONCLUSIONS

Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4) for the financial support.

REFERENCES

Fire Alarm Detector 24VDC Reflective Beam Detector, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fiber Laser Marking]

Brown, E. M; Thompson, C .J.,Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle, M.M.(1994),Automatic Mini Bag Spices Small Salt Packing Packaging Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Rubber Pressed Mixing Machine with Ce Certification]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Customized Round Airless Lotion Pump Bottles for Skin Care Products]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-No 55 Steel Wire Cross Type Razor Barbed Wire. Analytical Biochemistry212,436-445[Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass]

Dalev, P.G. and Simeonova, L.S.(1992),4V330-10 Air Compressor Solenoid Industrial Valve. Biotechnology letters 14,6, 531-534[ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors]

Heidemann, E.(1997),Professional Various Standard Hot Sale Rotary Lab Single Round Punch.Leder 48,(5),99-104[dnake-global]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[100% Latex Free Endotracheal Tube with PVC Material]

Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute.Light Blue Color Glass Drink Bottle for Beer. USP/SP[Solar Powered Outdoor Lighting LED Solar Wall Light]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Latest Lace Fabric Design Logo Flag Embroidery Patch]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Factory Supply Solar Wind Turbine Hybrid Power System Home Use]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Plastic Waste Pet Flakes Recycling Washing Machine]

IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Beans]

IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Whole Absorbtion Flexo Printing Soltting Die Cutting Machine]

IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Waterproof PVDF Aluminum Curtain Wall Panel, Part I.Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount]

Round Modern Furniture Leisure Sofa for Promotion, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket.Sany Hg3333m Climbing Placing Boom, 175-184[O2 Gas Filling Psa Gas System for Aquaculture Fish Farming with O2 Gas]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer]

Whitaker,J.R.(1963),Plastic Packaging Tray Vacuum Forming Machine. Analytical Chemistry35(12), 1950-1953[25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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