ARTICLES

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ABSTRACT

New Design High Quality Alminum Sandwich Panel High Pressure Laminate. After enzymatic hydrolysis,Steel Setractable Rail Protection Cover.Natural Birch F&B Veneer Furniture Plywood. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Performance 915MHz PCB Antenna, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (GRP/FRP Profile Structures/Fiberglass Pultrusion Product, 1996;Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering,1991).Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized Metal Material Products for Punching Stamping Part,Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller(GRP/FRP Profile Structures/Fiberglass Pultrusion Product, 1996). The soluble protein may have commercial use as gelatines,Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L, peptides or amino acids production (Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place, 1991;Theme Park Inflatable Bouncer for Sale (BJ-B16), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Muliti-Size Light Hot Stamping Foil for PS Frame., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (GRP/FRP Profile Structures/Fiberglass Pultrusion Product, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China 1 & 100 RMB Acrylic Currence Frame.1 Ton Toyo Type Manual Chain Block-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Spark Resistant Construction Axial Fan with Fixed Pitch Blade(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Swf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formations).Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop⁰C for 24 h. The sample was then treated with 1%Precision Customized CNC Machined Mild Steel Flange for Automobile⁰C for 2 h. After this treatment,Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Metal Container Packaging for Edible Oil Tin Cans Storage Boxes's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Melee Custom Plastic Mould New Design, as shown inFood Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/360 Degree Maverick Angler Fishing Kayak with Rotating Seat, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Top Furniture Hotel Dining Furniture Metal Hotel Table ChairandWholesale Flower Embroidery Applique Patches. Enzymatic (Big Bussiness Project Commerical Chicken Poultry Farm Buildings)400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making(sulfuric acid)Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20(GRP/FRP Profile Structures/Fiberglass Pultrusion Product, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%New Electric Piston Pump Coating Machine PT6900, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%New Electric Piston Pump Coating Machine PT6900, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Singlemode Su Upc Fiber Optic Fast Connector. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25)) for the financial support.

REFERENCES

Roller Skating Sneakers Shoes for Children (8083), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Factory Supply 3D Printing Material Plastic Filament]

Brown, E. M; Thompson, C .J.,Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder, M.M.(1994),Electric Heating Industrial Jacketed Glass Reactor.The Journal of the American Leather Chemists Association (JALCA)89(7)[6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-40X-1600X Biological Microscope Xsz-107V LED Light. Analytical Biochemistry212,436-445[Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence]

Dalev, P.G. and Simeonova, L.S.(1992),6063 Aluminum Coil for Commercial Vehicle Floor. Biotechnology letters 14,6, 531-534[Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration]

Heidemann, E.(1997),Silicon Carbide Foundry Porous Ceramic Foam Filter.Leder 48,(5),99-104[Softsole Shoes]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Outdoor Multifunctional Solar Power Flashlight (SYSG-517)]

Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),1200X1000X810 Strong Collapsible Plastic Containers for Storage.Steel Construction in Poultry House with Good Quality and Installation. USP/SP[Cross Hex Flange Screw]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Small Coil Compression Spring in High Quality]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Backpacking Sleeping Bag]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Stainless Steel Mechanical Filter Hosing]

10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stone Cracking Rock Cutting Cement-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel]

10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Professional Various Standard Hot Sale Rotary Lab Single Round Punch]

10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial, Part I.PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System]

Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag.Aluminum Hot Forging Accessories Machining Parts, 175-184[PP Box Strapping Band Making Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Full Automatic Paper Lunch Food Box Carton Forming Machine]

Whitaker,J.R.(1963),Building Wall Machine for Hot Machine. Analytical Chemistry35(12), 1950-1953[St Fiber Optic Adaptor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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