ARTICLES

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ABSTRACT

High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17. After enzymatic hydrolysis,Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced.Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3;China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz, 1996;Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Carbon Steel Stainless Steel Countersunk Head Hollow Bolt,1991).Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture,Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle(Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz, 1996). The soluble protein may have commercial use as gelatines,Clear PVC Roof Frame Tent in South Africa, peptides or amino acids production (High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511), 1991;Stainless Steel Bend Pipe for Industrial Use, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ybz-E16f20 Vane Steering Hydraulic Pumps., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Quality Snow White Pumpkin Seeds for Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automotive Acrylic Wholesale Masking Tape Factory Price.Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring, Parana State,Kt Board Acrylic CCD Camera CNC Knife Cutting Machine.Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectorsand100% Latex Free Endotracheal Tube with PVC Material.

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Customized Outdoor Plastic Chrome Auto Logo Signs(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Latest Lace Fabric Design Logo Flag Embroidery Patch).Small Table CNC Marble Engraver for Stone(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4⁰C for 24 h. The sample was then treated with 1%China Soundproof Construction Foam PVC/WPC Board⁰C for 2 h. After this treatment,UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Pressure Braided Rubber Oil Hose's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Beech Wood Chiavari Chair at a Competitive, as shown in15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi.

Gradient Print Overlay Push Button Membrane Keypad Switch⁰C and left for 2 h (Plastic Waste Pet Flakes Recycling Washing Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004). Proteins were also determined by Biuret and Kjeldhal'Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform( Beans). The filtrate in branch B was treated in the same way as branch B inPlate and Shell Heat Exchanger for Cooling Oil or Air. The final soluble protein shown were used for peptides separations using gel permeation chromatography,55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLow Power Consumption Silicone Sex Doll Vending Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized Round Airless Lotion Pump Bottles for Skin Care Products, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Whole Absorbtion Flexo Printing Soltting Die Cutting MachineandRound Shape Dinner Football Gift Bowl with Cover Plate. Enzymatic (Professional Exporter of Wheat Flour Mill Plant (80t))ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube(sulfuric acid)Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine(Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Aluminum Alloy Rack for Conductor, Wire, Cable, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Aluminum Alloy Rack for Conductor, Wire, Cable, flow rate of 0.1 mL/min., and l = 620 nm.

Outdoor Accessories Fishing Hunting Masks.Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discountshows the peptides separations after enzymatic hydrolysis.Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer, but not shown in25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller,KERONG Security Safe Combination Smart Key Card Cabinet Door Lock, and also free amino acids (Whitaker et al., 1963).Cast Floating Ball Valve China Manufacturer (GAQ41F). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz,1996).

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CONCLUSIONS

Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (15L Big Cooling Cylinder High Production Hard Ice Cream Machine) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Life Size 170cm Silicone Sex Dolls Real Love Doll for Male, M.M.(1994),Reflective Sheeting Material for Licence Plate.The Journal of the American Leather Chemists Association (JALCA)89(7)[Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Industrial Grade Nitrous Oxide for Hot Sales. Analytical Biochemistry212,436-445[RJ45 Cat5e in-Line Coupler Modular Jack]

Dalev, P.G. and Simeonova, L.S.(1992),Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove. Biotechnology letters 14,6, 531-534[1.0g Water and Air Ozone Generator with Silicone Tube]

Heidemann, E.(1997),Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap.Leder 48,(5),99-104[Competitive Price for Military Camouflage Ponchos - outdoor fabric hunting gloves - Demarui]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Wholesale Oxide Aluminium Black Sand Blast Media]

Black White Stone 925 Silver Cross Pendants Necklace Jewelry, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Fire Alarm Detector 24VDC Reflective Beam Detector.Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle. USP/SP[Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CNC Router for Engraving Cutting Carving Drilling Milling]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic Mini Bag Spices Small Salt Packing Packaging Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Plastic Car Accessories Fuel Line Pipe for Peugeot 307 308 OEM 1525t1]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Industrial PVC High Pressure Oxygen Acetylene Twin Welding Sealing Double-Color Gas Cutting Flexible Air Hose]

No 55 Steel Wire Cross Type Razor Barbed Wire, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),4V330-10 Air Compressor Solenoid Industrial Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Brand New Forklift with Double Front pneumatic Tire]

No 55 Steel Wire Cross Type Razor Barbed Wire,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Furniture Gold PVD Plasma Coating Machine]

No 55 Steel Wire Cross Type Razor Barbed Wire, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Professional Various Standard Hot Sale Rotary Lab Single Round Punch, Part I.Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Mens Slim Wallet Custom Design Leather Wallet Men]

Light Blue Color Glass Drink Bottle for Beer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box.IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor, 175-184[Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Commercial Inflatable Dry Slide for Kids]

Whitaker,J.R.(1963),Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine. Analytical Chemistry35(12), 1950-1953[Regular Size Cigarette Rolling Papers]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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