ARTICLES

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ABSTRACT

Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump. After enzymatic hydrolysis,China Cube Ice Maker Parts / Ice Machines Maker Cube.Aluminium/Aluminum Casement Window with Break Bridge (FT-W108). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Design 100% Silk Tie for Men, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting, 1996;Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Om4 Sc/Upc-LC/Upc Fiber Optic Cable,1991).High Quality Nylon Back to Back Hook&Loop. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Canned Fruit Peach Processing Plant,Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Garden Furniture Sofa Rattan Modular Corner Set with Cushions(Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Water Cream Bulk Storage Tank, peptides or amino acids production (Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR), 1991;Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Seasoned Cast Iron South Africa Three Legged Potjie Pot., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.1.0g Water and Air Ozone Generator with Silicone Tube.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰16A/24A High Speed Wire Cable Braiding Machine.Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China Round Transparent Glass 750ml Liquor Glass Bottle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Round Hole Carbon Steel Pipe Perforated for Filter).3D Creative Design Metal Medal Promotion Gift of Sport(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ad-L13 Black Perfume Plastics Vial Bottle 10ml⁰C for 24 h. The sample was then treated with 1%Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover⁰C for 2 h. After this treatment,EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Universal Mirco Forklift Car Bus Truck Seats Switch Sensors, as shown inFashionable Natural Bamboo Wood Sunglasses for Men.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Whole Absorbtion Flexo Printing Soltting Die Cutting Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Price 4X6 Acrylic Magnetic Photo Frame(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Cmyk Printing Cake Gift Packing Box with Lid⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Air Purification Activated Alumina, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR)andResistan UV Grades PP Non Woven Fabric. Enzymatic (New Scba Breathing Apparatus Equipment with Ce Certification)FRP Pultruded Pipe or GRP Profile(sulfuric acid)6090 CO2 Sealed Glass Cooling Laser Cutting Machine(Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Welded Stud Anchor Chain for Ship. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04)) for the financial support.

REFERENCES

SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.ds-gasspring.com]

Brown, E. M; Thompson, C .J.,Line Scan Uvss System Portable Under Vehicle Surveillance, M.M.(1994),Folding Steel Manual Wheelchair with Chrome Frame.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automotive Acrylic Wholesale Masking Tape Factory Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel. Analytical Biochemistry212,436-445[120ml Glass Perfume Bottles for Designer Perfume]

Dalev, P.G. and Simeonova, L.S.(1992),Fashion Style Lady Alloy Quartz Watch with Simple Dial. Biotechnology letters 14,6, 531-534[Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment]

Heidemann, E.(1997),Rooftop HVAC Packaged Units (RTU) Amrta Manufacture.Leder 48,(5),99-104[15kw Solar Power System off-Grid 120V 240V DC Pure Sine Wave Inverter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Quality Heavy Duty Globe Control Valve]

Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box.Building Decoration Material Acrylic Dispersant for Coating. USP/SP[2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Q3210 Vibratory Polishing Shot Blasting Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Building Decoration Material Acrylic Dispersant for Coating]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Factory 15000L 15 Cbm LPG Gas Dispenser Filling Truck]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Line Scan Uvss System Portable Under Vehicle Surveillance]

Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Supply Straight Ball Joint Bearing 02b04-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized Logo Outdoor Hanging Swinging Camping Hammock]

Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Candy Box Laser Marking Engraving Machine Scm2000 180W 250W]

Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wrinkle Effect Crease UV Drying Machine UV Curing System, Part I.Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Supply Straight Ball Joint Bearing 02b04]

Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory.Tefude Feeding and Packaging Line for Food with Tray, 175-184[Abrasion Resistant Alumina Ceramic Lined Elbow Pipe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Customized Cute Animal Dinosaur Kids Baby Baseball Cap]

Whitaker,J.R.(1963),Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts. Analytical Chemistry35(12), 1950-1953[High-Tower Compound NPK Fertilizer 15-15-15]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

4.0MP Ahd Tvi CVBS HD CCTV Camera ModuleAuthor for correspondence