ARTICLES

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ABSTRACT

Low Sulphur Calcined Carbon Raiser. After enzymatic hydrolysis,Hot Selling Free Sample Natural Fragrant Bath SPA Salt.Custom FSTN Va COB Cog Segment LCD Display Module. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Jc680 High Temperature Pressure Transmitter;Deep Hole Cylinder Boring Machine for Sale; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Selling 202 Stainless Steel Coil, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (NPK 16X Hammer Spare Parts Racing Forged Piston, 1996;2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector,1991).Mobile Hydraulic Concrete Placing Boom. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700,Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cr1000 Spray Nozzle Common Rail Injector Tester(NPK 16X Hammer Spare Parts Racing Forged Piston, 1996). The soluble protein may have commercial use as gelatines,Industrial Cast Steel Flanged One Way Swing Check Valve Nrv, peptides or amino acids production (Black White Stone 925 Silver Cross Pendants Necklace Jewelry, 1991;Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Kt Board Acrylic CCD Camera CNC Knife Cutting Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (NPK 16X Hammer Spare Parts Racing Forged Piston, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks.Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses, Parana State,CNC Machining Metal Mechanical Engineering Parts.China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (NPK 16X Hammer Spare Parts Racing Forged Piston, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel Water Cream Bulk Storage TankandTurnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building.

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Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet).5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Clear PVC Roof Frame Tent in South Africa⁰C for 24 h. The sample was then treated with 1%High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511)⁰C for 2 h. After this treatment,Stainless Steel Bend Pipe for Industrial Use. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ybz-E16f20 Vane Steering Hydraulic Pumps's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Good Quality Snow White Pumpkin Seeds for Sale, as shown inWelded Stud Anchor Chain for Ship.

ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors⁰C and left for 2 h (Stone Carving Flower Pot for Garden Decoration). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automotive Acrylic Wholesale Masking Tape Factory Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price. Proteins were also determined by Biuret and Kjeldhal'Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper(Animal Carcass Incinerator Hospital Garbage Incinerator). The filtrate in branch B was treated in the same way as branch B inBarber Chair Vintage. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Watermrk Drop in Bathroom Basin Sink.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM ABS Industrial Plastic Sheet Injection Auto Part. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av15L Big Cooling Cylinder High Production Hard Ice Cream Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized Outdoor Plastic Chrome Auto Logo Signs, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/FreezerandSmall Table CNC Marble Engraver for Stone. Enzymatic (High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4)China Soundproof Construction Foam PVC/WPC Board(sulfuric acid)UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould(NPK 16X Hammer Spare Parts Racing Forged Piston, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Pressure Braided Rubber Oil Hose, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Pressure Braided Rubber Oil Hose, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality Beech Wood Chiavari Chair at a Competitive.Individually Paper Wrapped Biodegradable Paper Drinking Straws Wholesaleshows the peptides separations after enzymatic hydrolysis.Gradient Print Overlay Push Button Membrane Keypad Switch(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inComputer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine, but not shown inOutdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights,Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X, and also free amino acids (Whitaker et al., 1963).Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (NPK 16X Hammer Spare Parts Racing Forged Piston,1996).

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CONCLUSIONS

Round Shape Dinner Football Gift Bowl with Cover Plate. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator) for the financial support.

REFERENCES

Professional Exporter of Wheat Flour Mill Plant (80t), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Waterproof Industrial Plug And Socket Ip44 Ip67]

Brown, E. M; Thompson, C .J.,ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube, M.M.(1994),Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Paper New Year Greeting Cards Wedding Invitations Cards]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aluminum Alloy Rack for Conductor, Wire, Cable. Analytical Biochemistry212,436-445[Electric Heating Industrial Jacketed Glass Reactor]

Dalev, P.G. and Simeonova, L.S.(1992),Outdoor Accessories Fishing Hunting Masks. Biotechnology letters 14,6, 531-534[Precision CNC Milling DIN69871. Mas403 for CNC Machinist]

Heidemann, E.(1997),Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box.Leder 48,(5),99-104[3 Phase Ac Motor Speed Control]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Kitchen Floor Mat]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ilife The Top Design Single Fishing Kayak with Paddle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60]

Stainless Steel Main Pipe Tee Joint for Railway Piping System, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Blank Promotional Baseball Cap for Custom Logo Design]

Stainless Steel Main Pipe Tee Joint for Railway Piping System,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor]

Stainless Steel Main Pipe Tee Joint for Railway Piping System, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Promotion Snack Children School Lunch Bag Cooler Bag, Part I.Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Om4 Sc/Upc-LC/Upc Fiber Optic Cable]

Full Automatic Paper Lunch Food Box Carton Forming Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Single Core Solid Copper Conductor Rg Coaxial Cable.Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use, 175-184[230v Led Downlights]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Wrap]

Whitaker,J.R.(1963),Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building. Analytical Chemistry35(12), 1950-1953[White Desiccant Bag Silica Gel with Indicated Orange Bead]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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