ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Ltmg Classic Product 6 Ton Wheel Front Loader for Sale. After enzymatic hydrolysis,Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror.Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Household Family Medical Emergency Electronic Digital Thermometer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Die Cast Iron Worm Gear Speed Reduce Gearbox, 1996;19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Couple Watches Steel Material Gift Watch OEM in China,1991).Glass Tea Sugar Coffee Storage Jar with Screw Cap. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Circular DC Electro Lifting Magnet for Lifting Scraps,Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Particle Board Manufacturer, Melamine/Plain Chipboard(Die Cast Iron Worm Gear Speed Reduce Gearbox, 1996). The soluble protein may have commercial use as gelatines,CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part, peptides or amino acids production (Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G), 1991;Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.120ml Glass Perfume Bottles for Designer Perfume.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Agriculture Hay Silage Wrapping Film. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Die Cast Iron Worm Gear Speed Reduce Gearbox, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer.Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:16oz Insulated Cardboad Cup Sleeve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant, Parana State,Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration.LED Rectangular Recessed Linear Lamps for Bank and Car Sale, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Die Cast Iron Worm Gear Speed Reduce Gearbox, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFood Grade Customized Metal Candy Can Square Mint Tin BoxandJqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving.

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Salon Equipment Hair Clipper(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ybz-E16f20 Vane Steering Hydraulic Pumps).Flat Conveyor Belt Steel Idler Frame for Roller(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6⁰C for 24 h. The sample was then treated with 1%Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle⁰C for 2 h. After this treatment,Durable TPR Pet Molar Toy with Colorful Rope. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Chromium Oxide Green 99% for Construction and Plastics, as shown inColored Silicone NBR O-Rings Seals.

Zs Round Vibrating Sieve⁰C and left for 2 h (Electronic Foldable for Patient Treatment Ent Unit Chair). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainless Steel Tactile Paving Blind Road Stud. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,70L Lab Use Refrigerated Circulator Chiller Factory Price. Proteins were also determined by Biuret and Kjeldhal'Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500)(Stainless Steel Balustrade/Pillar/Column). The filtrate in branch B was treated in the same way as branch B in22mm G80 Black Alloy Steel Chain G80 Hoist Lifting Chain. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Roller Hearth Heating Furnace for Gas Cylindrer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%custom design MIFARE DESFire EV2 2K plastic RFID loyalty card. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel DIN7981 Pan Head Self Tapping Screw, pH 3.25., mobile phase B: buffer solution of 0.01 mol/19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Precision Investment Casting Stainless Steel 304 Wire Rope Hook, V_e, and K_avcalculated as discussed previously.AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture,Custom Die Casting Aluminium Product for Connector Usage. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated) (Reis et al.,1989;Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G).,1991;19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting.,1991).Stainless Steel Balustrade/Pillar/Column(1992), were lower, except for cystine and tyrosine,Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller.

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CONCLUSIONS

PP/Pet Woven Geotextile for Road Construction. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wood Fireplace Double Skin Chimney Flue Pipes) for the financial support.

REFERENCES

Lamella Clarifier in Industrial Wastewater Treatment, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Small Handheld Thermal Camera for Hunting]

Brown, E. M; Thompson, C .J.,FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering, M.M.(1994),Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED.The Journal of the American Leather Chemists Association (JALCA)89(7)[41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[HOWO Tipper Truck Price with Manufacture Factory Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights. Analytical Biochemistry212,436-445[Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell]

Dalev, P.G. and Simeonova, L.S.(1992),Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick. Biotechnology letters 14,6, 531-534[Square Shape Glass Candle Holder for One Piece Tealight Candle]

Heidemann, E.(1997),Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts.Leder 48,(5),99-104[Wholesale Cheap Iron on Sublimation Printing Woven Label Patches]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Selling Solidwood Viola Wholesale Price Viola]

Hot Selling Solidwood Viola Wholesale Price Viola, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),IS Type Cast Iron Chemical Industrial Pump.Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine. USP/SP[Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Grooved Quartz Plate Deep Processing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Full Port Small Automatic Electronic Plumbing Ball Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.wanyingtools.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[20 Ton Small Vertical Plastic Injection Molding Machine]

Hot Sale CNC Router Machine for Wood Cutting (1325), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[The Big Diameter Carbide Tipped Hole Saw]

Hot Sale CNC Router Machine for Wood Cutting (1325),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter]

Hot Sale CNC Router Machine for Wood Cutting (1325), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:50g Body Luxury King Crown Cosmetic Containers, Part I.Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Sexy Crystal Self Adhesive Resuable Nipple Cover]

Mini Succulent Planters, Cactus Pots with Wooden Cement Pot, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Paper Egg Box Forming Molding Production Line/ Egg Tray Machine.4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine, 175-184[Agricultural Bale Net]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[NPK+Te Fertilizer]

Whitaker,J.R.(1963),Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330). Analytical Chemistry35(12), 1950-1953[Cold-Spray Road Line Marking Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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