ARTICLES

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ABSTRACT

Glass Tea Sugar Coffee Storage Jar with Screw Cap. After enzymatic hydrolysis,Circular DC Electro Lifting Magnet for Lifting Scraps.Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Particle Board Manufacturer, Melamine/Plain Chipboard;CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert, 1996;Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (120ml Glass Perfume Bottles for Designer Perfume,1991).Agriculture Hay Silage Wrapping Film. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer,Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,16oz Insulated Cardboad Cup Sleeve(Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert, 1996). The soluble protein may have commercial use as gelatines,Large Outdoor Simple Gazebo Beer Festival Marquee Tent, peptides or amino acids production (HOWO Tipper Truck Price with Manufacture Factory Price, 1991;Wood Fireplace Double Skin Chimney Flue Pipes, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality 304 Stainless Steel Sheet for Home Decoration Material., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-LED Rectangular Recessed Linear Lamps for Bank and Car Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Salon Equipment Hair Clipper.Flat Conveyor Belt Steel Idler Frame for Roller-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Zs Round Vibrating Sieve, Parana State,High Quality Couple Watches Steel Material Gift Watch OEM in China.Stainless Steel Tactile Paving Blind Road Stud, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAcid Water Pulse Output Liquid Orifice Type Flow MeterandGc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane.

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70L Lab Use Refrigerated Circulator Chiller Factory Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Aluminum Window System Thermal Break Strip (HK Stype35.3mm)).Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Roller Hearth Heating Furnace for Gas Cylindrer⁰C for 24 h. The sample was then treated with 1%custom design MIFARE DESFire EV2 2K plastic RFID loyalty card⁰C for 2 h. After this treatment,Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hotel Card Door Lock Access Control Safe Door Lock's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer, as shown inAuto Parts Cross Propeller Shaft for Mercedes Benz 12062X.

Stainless Steel DIN7981 Pan Head Self Tapping Screw⁰C and left for 2 h (Die Casting Zinc Alloy Badge Use for Car). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax. Proteins were also determined by Biuret and Kjeldhal'Dark Green Decorative Garden Welded Wire Mesh Fencing(Ampoule Sealer Machine Hydrogen Oxygen Gas Generator). The filtrate in branch B was treated in the same way as branch B inHot Home Decoration Small Romantic Luminary Fire Free Flickering LED Tea Lights. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Csm DIY Microscope for Kids/Toy Microscope.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Oak Veneer Laminated Plywood for Decoration. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Speed HDMI Cable Wholesale HDMI with Ethernet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Women′s Suit in Reasonable Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Custom Grooved Quartz Plate Deep Processing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring) for the financial support.

REFERENCES

Hot Sale CNC Router Machine for Wood Cutting (1325), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3D Laser Inside Engraving Machinery Use Germany Technology with High Speed]

Brown, E. M; Thompson, C .J.,Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine, M.M.(1994),50g Body Luxury King Crown Cosmetic Containers.The Journal of the American Leather Chemists Association (JALCA)89(7)[Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[900-1800MHz GSM PCB Antenna]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men. Analytical Biochemistry212,436-445[Q-Sub Subwoofer Professional Passive Speaker Loud Speaker Woofer]

Dalev, P.G. and Simeonova, L.S.(1992),Mini Succulent Planters, Cactus Pots with Wooden Cement Pot. Biotechnology letters 14,6, 531-534[Neodymium Magnet Pot Assembly with Strong Weight]

Heidemann, E.(1997),Paper Egg Box Forming Molding Production Line/ Egg Tray Machine.Leder 48,(5),99-104[Garden Using Waterproof LED Pinecone Fairy String Light with Ce RoHS IP44]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Clear PVC Roof Frame Tent in South Africa]

Die Cast Iron Worm Gear Speed Reduce Gearbox, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine.Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330). USP/SP[China Soundproof Construction Foam PVC/WPC Board]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sale CNC Router Machine for Wood Cutting (1325)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CNC Metal Grinding Machine Tool Mk2120: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Customized Wire Harness for Various Application]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Edge Gluer Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men]

Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale]

Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Best Price 1.4507/S32550/Alloy 2507 Stainless Steel Pipe]

Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM, Part I.12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing]

Hydraulic Electric Stationary Car Platform Scissor Elevator Lift, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building.Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal, 175-184[Hydraulic Fitting Nut]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle]

Whitaker,J.R.(1963),Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo. Analytical Chemistry35(12), 1950-1953[Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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