ARTICLES

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ABSTRACT

Samsung LTI550HN16 1920X1080P monitor screen LCD video wall. After enzymatic hydrolysis,Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts.Household Family Medical Emergency Electronic Digital Thermometer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Die Cast Iron Worm Gear Speed Reduce Gearbox;19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Couple Watches Steel Material Gift Watch OEM in China, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Glass Tea Sugar Coffee Storage Jar with Screw Cap, 1996;Circular DC Electro Lifting Magnet for Lifting Scraps., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts,1991).Particle Board Manufacturer, Melamine/Plain Chipboard. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part,Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert(Glass Tea Sugar Coffee Storage Jar with Screw Cap, 1996). The soluble protein may have commercial use as gelatines,Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps, peptides or amino acids production (120ml Glass Perfume Bottles for Designer Perfume, 1991;Agriculture Hay Silage Wrapping Film, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-16oz Insulated Cardboad Cup Sleeve. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Glass Tea Sugar Coffee Storage Jar with Screw Cap, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Large Outdoor Simple Gazebo Beer Festival Marquee Tent.HOWO Tipper Truck Price with Manufacture Factory Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wood Fireplace Double Skin Chimney Flue Pipes(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale CNC Router Machine for Wood Cutting (1325)).Durable TPR Pet Molar Toy with Colorful Rope(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring⁰C for 24 h. The sample was then treated with 1%Chromium Oxide Green 99% for Construction and Plastics⁰C for 2 h. After this treatment,Zs Round Vibrating Sieve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Tactile Paving Blind Road Stud's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)70L Lab Use Refrigerated Circulator Chiller Factory Price, as shown inCustomized Wire Harness for Various Application.

Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500)⁰C and left for 2 h (Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Roller Hearth Heating Furnace for Gas Cylindrer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,custom design MIFARE DESFire EV2 2K plastic RFID loyalty card. Proteins were also determined by Biuret and Kjeldhal'Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016(Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale). The filtrate in branch B was treated in the same way as branch B in4 Pin 10 Pin Molex 2510 Flat Cable Connectors. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hotel Card Door Lock Access Control Safe Door Lock.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel DIN7981 Pan Head Self Tapping Screw(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Dark Green Decorative Garden Welded Wire Mesh Fencing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Csm DIY Microscope for Kids/Toy Microscope, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality Women′s Suit in Reasonable Price.High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processingshows the peptides separations after enzymatic hydrolysis.Pet Short Fiber Non Woven Geotextile for Landfill Filtration(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle, but not shown inHot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine,Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof, and also free amino acids (Whitaker et al., 1963).Competitive Price Sodium Gluconate as Concrete Admixture. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Glass Tea Sugar Coffee Storage Jar with Screw Cap,1996).

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Stainless Steel Balustrade/Pillar/Column, V_e, and K_avcalculated as discussed previously.Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller,PP/Pet Woven Geotextile for Road Construction. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Electronic Foldable for Patient Treatment Ent Unit Chair) (Reis et al.,1989;120ml Glass Perfume Bottles for Designer Perfume.,1991;Circular DC Electro Lifting Magnet for Lifting Scraps.,1991).Lamella Clarifier in Industrial Wastewater Treatment(1992), were lower, except for cystine and tyrosine,FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering.

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CONCLUSIONS

Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (LED Rectangular Recessed Linear Lamps for Bank and Car Sale) for the financial support.

REFERENCES

Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hollywood Mirror Argos]

Brown, E. M; Thompson, C .J.,Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick, M.M.(1994),Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Foska Mini Size High Quality Heavy Duty Stapler]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Performance 915MHz PCB Antenna]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-IS Type Cast Iron Chemical Industrial Pump. Analytical Biochemistry212,436-445[High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine. Biotechnology letters 14,6, 531-534[Custom Welding Stainless Steel Sheet Metal Welding Parts]

Heidemann, E.(1997),Custom Grooved Quartz Plate Deep Processing.Leder 48,(5),99-104[Fashion Hoop Gold Plated Earring CZ Stone Jewelry for Women]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Quick Connect Camlock and Grooved Couplings Quick Fitting Type D]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Laser ID Card Rigid PET Printing Sheet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-50g Body Luxury King Crown Cosmetic Containers: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Hamburgers]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Non-Slip Anti-Fatigue Comfort Rubber Floor Mat]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated]

Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Mini Succulent Planters, Cactus Pots with Wooden Cement Pot-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Condition Hongyu Polystyrene Panel CNC Hot Wire Contour Foam Cutte]

Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary]

Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Paper Egg Box Forming Molding Production Line/ Egg Tray Machine, Part I.4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45)]

Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CNC Metal Grinding Machine Tool Mk2120.Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket, 175-184[Outdoor Indoor Furniture Waterproof Speaker Bluetooth]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[HOWO Tipper Truck Price with Manufacture Factory Price]

Whitaker,J.R.(1963),Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit. Analytical Chemistry35(12), 1950-1953[Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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